Cholate and pH Reduce Interference by Sodium Dodecyl Sulfate in the Determination of DNA with Hoechst

The use of the fluorescent dye 33258 Hoechst (Hoe) to quantitatively determine DNA in cell culture in the presence of lysing agents like sodium dodecyl sulfate (SDS) is limited by the masking effect of high levels of nonspecific fluorescence, caused by the binding of Hoe to micelles. The masking eff...

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Veröffentlicht in:	Analytical biochemistry 1994-12, Vol.223 (2), p.299-305
Hauptverfasser:	Bester, M.J., Potgieter, H.C., Vermaak, W.J.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bisbenzimidazole Cattle Cetrimonium Compounds Cholic Acid Cholic Acids DNA - analysis DNA - standards DNA, Neoplasm - analysis Humans Hydrogen-Ion Concentration Methods Micelles Reference Standards Sensitivity and Specificity Sodium Dodecyl Sulfate Spectrometry, Fluorescence Tumor Cells, Cultured - chemistry
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