Studies on Steroids in Fetuses and Neonates : Identification of 16-Dehydropregnenolone in the Circulation of Pre-term Neonates

Unconjugated steroids were extracted with dichloromethane from the serum of pre-term neonates. Conjugated steroid fraction in the residue was separated and purified by high performance liquid chromatography. The purified steroid fraction was subjected to enzyme (arylsulfatase from Helix pomatia) or...

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Veröffentlicht in:	Biological & pharmaceutical bulletin 1994/11/15, Vol.17(11), pp.1501-1504
Hauptverfasser:	KOBAYASHI, Yoshiharu, TAGAWA, Noriko, SAIKI, Kayoko, WATANABE, Fukuko
Format:	Artikel
Sprache:	eng
Schlagworte:	16-dehydropregnenolone Arylsulfatases - metabolism Biological and medical sciences Blood Chemical Analysis Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry GC/FT-IR GC/MS Gestational Age Humans Hydrogen-Ion Concentration Hydrolysis Infant, Newborn Infant, Premature - blood Methylene Chloride - chemistry pre-term neonate Pregnenolone - analogs & derivatives Pregnenolone - blood Pregnenolone - isolation & purification Pregnenolone - pharmacokinetics Spectroscopy, Fourier Transform Infrared Steroid hormones. Cholecalciferol derivatives Vertebrates: endocrinology
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