Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording
We describe fabrication methods and the characterisation and use of extracellalar microelectrode arrays for the detection of action potentials from neurons in culture. The 100 μm2 platinised gold microelectrodes in the 64 electrode array detect the external current which flows during an action poten...
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| Veröffentlicht in: | Journal of neuroscience research 1995-10, Vol.42 (2), p.266-276 |
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| container_title | Journal of neuroscience research |
| container_volume | 42 |
| creator | Breckenridge, L. J. Wilson, R. J. A. Connolly, P. Curtis, A. S. G. Dow, J. A. T. Blackshaw, S. E. Wilkinson, C. D. W. |
| description | We describe fabrication methods and the characterisation and use of extracellalar microelectrode arrays for the detection of action potentials from neurons in culture. The 100 μm2 platinised gold microelectrodes in the 64 electrode array detect the external current which flows during an action potential with S:N ratios of up to 500:1, giving a maximum recorded signal of several millivolts. The performance of these electrodes is enhanced if good sealing of the cells over the electrodes is obtained and further enhanced if the electrodes and the cells lie in a deep groove in the substratum. The electrodes can be used for both recording and stimulation of activity in cultured neurons and for recording from multiple sites on a single cell. The use of such electrodes to obtain recordings from invertebrate neurons is described. The particular advantages of these electrodes, their long term stability, non‐invasive nature, high packing density, and utility in stimulation, are demonstrated. © 1995 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jnr.490420215 |
| format | Article |
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J. ; Wilson, R. J. A. ; Connolly, P. ; Curtis, A. S. G. ; Dow, J. A. T. ; Blackshaw, S. E. ; Wilkinson, C. D. W.</creator><creatorcontrib>Breckenridge, L. J. ; Wilson, R. J. A. ; Connolly, P. ; Curtis, A. S. G. ; Dow, J. A. T. ; Blackshaw, S. E. ; Wilkinson, C. D. W.</creatorcontrib><description>We describe fabrication methods and the characterisation and use of extracellalar microelectrode arrays for the detection of action potentials from neurons in culture. The 100 μm2 platinised gold microelectrodes in the 64 electrode array detect the external current which flows during an action potential with S:N ratios of up to 500:1, giving a maximum recorded signal of several millivolts. The performance of these electrodes is enhanced if good sealing of the cells over the electrodes is obtained and further enhanced if the electrodes and the cells lie in a deep groove in the substratum. The electrodes can be used for both recording and stimulation of activity in cultured neurons and for recording from multiple sites on a single cell. The use of such electrodes to obtain recordings from invertebrate neurons is described. The particular advantages of these electrodes, their long term stability, non‐invasive nature, high packing density, and utility in stimulation, are demonstrated. © 1995 Wiley‐Liss, Inc.</description><identifier>ISSN: 0360-4012</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/jnr.490420215</identifier><identifier>PMID: 8568928</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Action Potentials ; Animals ; Axons - physiology ; Axons - ultrastructure ; Cell Culture Techniques - instrumentation ; cell/electrode seal ; Cells, Cultured ; Electric Impedance ; Electrophysiology - instrumentation ; extracellular microelectrode arrays ; Ganglia, Invertebrate - cytology ; Leeches - physiology ; Lymnaea - physiology ; Microelectrodes ; multisite recording ; Nerve Net - physiology ; neuronal networks ; Neurons - physiology ; Neurons - ultrastructure</subject><ispartof>Journal of neuroscience research, 1995-10, Vol.42 (2), p.266-276</ispartof><rights>Copyright © 1995 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4405-aba66c77587db34a395fcbe0d8652b86629447595703293777137106cde6dd203</citedby><cites>FETCH-LOGICAL-c4405-aba66c77587db34a395fcbe0d8652b86629447595703293777137106cde6dd203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjnr.490420215$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjnr.490420215$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8568928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Breckenridge, L. J.</creatorcontrib><creatorcontrib>Wilson, R. J. A.</creatorcontrib><creatorcontrib>Connolly, P.</creatorcontrib><creatorcontrib>Curtis, A. S. G.</creatorcontrib><creatorcontrib>Dow, J. A. T.</creatorcontrib><creatorcontrib>Blackshaw, S. E.</creatorcontrib><creatorcontrib>Wilkinson, C. D. W.</creatorcontrib><title>Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>We describe fabrication methods and the characterisation and use of extracellalar microelectrode arrays for the detection of action potentials from neurons in culture. The 100 μm2 platinised gold microelectrodes in the 64 electrode array detect the external current which flows during an action potential with S:N ratios of up to 500:1, giving a maximum recorded signal of several millivolts. The performance of these electrodes is enhanced if good sealing of the cells over the electrodes is obtained and further enhanced if the electrodes and the cells lie in a deep groove in the substratum. The electrodes can be used for both recording and stimulation of activity in cultured neurons and for recording from multiple sites on a single cell. The use of such electrodes to obtain recordings from invertebrate neurons is described. The particular advantages of these electrodes, their long term stability, non‐invasive nature, high packing density, and utility in stimulation, are demonstrated. © 1995 Wiley‐Liss, Inc.</description><subject>Action Potentials</subject><subject>Animals</subject><subject>Axons - physiology</subject><subject>Axons - ultrastructure</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>cell/electrode seal</subject><subject>Cells, Cultured</subject><subject>Electric Impedance</subject><subject>Electrophysiology - instrumentation</subject><subject>extracellular microelectrode arrays</subject><subject>Ganglia, Invertebrate - cytology</subject><subject>Leeches - physiology</subject><subject>Lymnaea - physiology</subject><subject>Microelectrodes</subject><subject>multisite recording</subject><subject>Nerve Net - physiology</subject><subject>neuronal networks</subject><subject>Neurons - physiology</subject><subject>Neurons - ultrastructure</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1P3DAQxa2Kim5pjz1W8olbYOLP-IhQd1sEW6ml6qWS5dgTZJpNqJ1Q-O8x2tWKE6fRaN776c0j5FMNJzUAO70d0okwIBiwWr4hixqMroQU-oAsgCuoBNTsHXmf8y0AGCP5ITlspGoMaxbkz1m4d8PkbjDTsaNzjsMN3USfxs61KXo3YaD4MCXnse_n3iWKPfopjaE4ujHROND7WHY64JzGwfU0oR9TKKAP5G3n-owfd_OI_Fp-uT7_Wl1-X307P7usvBAgK9c6pbzWstGh5cJxIzvfIoRGSdY2SjEjhJZGauDMcK11zXUNygdUITDgR-R4y71L478Z82Q3MT_ndQOOc7bF0UhpRBFWW2H5L-eEnb1LcePSo63BPrdpS5t232bRf96B53aDYa_e1Vfuenv_H3t8fB1mL9Y_XpJ3SWKe8GHvdOmvVZpraX-vV9ZcXVz_vFqu7Jo_AR66kEY</recordid><startdate>19951001</startdate><enddate>19951001</enddate><creator>Breckenridge, L. J.</creator><creator>Wilson, R. J. A.</creator><creator>Connolly, P.</creator><creator>Curtis, A. S. G.</creator><creator>Dow, J. A. T.</creator><creator>Blackshaw, S. E.</creator><creator>Wilkinson, C. D. W.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951001</creationdate><title>Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording</title><author>Breckenridge, L. J. ; Wilson, R. J. A. ; Connolly, P. ; Curtis, A. S. G. ; Dow, J. A. T. ; Blackshaw, S. E. ; Wilkinson, C. D. 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J.</creatorcontrib><creatorcontrib>Wilson, R. J. A.</creatorcontrib><creatorcontrib>Connolly, P.</creatorcontrib><creatorcontrib>Curtis, A. S. G.</creatorcontrib><creatorcontrib>Dow, J. A. T.</creatorcontrib><creatorcontrib>Blackshaw, S. E.</creatorcontrib><creatorcontrib>Wilkinson, C. D. W.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Breckenridge, L. J.</au><au>Wilson, R. J. A.</au><au>Connolly, P.</au><au>Curtis, A. S. G.</au><au>Dow, J. A. T.</au><au>Blackshaw, S. E.</au><au>Wilkinson, C. D. 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The performance of these electrodes is enhanced if good sealing of the cells over the electrodes is obtained and further enhanced if the electrodes and the cells lie in a deep groove in the substratum. The electrodes can be used for both recording and stimulation of activity in cultured neurons and for recording from multiple sites on a single cell. The use of such electrodes to obtain recordings from invertebrate neurons is described. The particular advantages of these electrodes, their long term stability, non‐invasive nature, high packing density, and utility in stimulation, are demonstrated. © 1995 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8568928</pmid><doi>10.1002/jnr.490420215</doi><tpages>11</tpages></addata></record> |
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| subjects | Action Potentials Animals Axons - physiology Axons - ultrastructure Cell Culture Techniques - instrumentation cell/electrode seal Cells, Cultured Electric Impedance Electrophysiology - instrumentation extracellular microelectrode arrays Ganglia, Invertebrate - cytology Leeches - physiology Lymnaea - physiology Microelectrodes multisite recording Nerve Net - physiology neuronal networks Neurons - physiology Neurons - ultrastructure |
| title | Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording |
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