Evaluation of Two-Dimensional Phosphopeptide Maps by Electrospray Ionization Mass Spectrometry of Recovered Peptides

Intracellular signaling pathways are to a large extent regulated by reversible protein phosphorylation of pathway components. To fully investigate the regulation of these pathways, it is often necessary to identify the sites of protein phosphorylation induced on individual components. The low abunda...

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Veröffentlicht in:	Analytical biochemistry 1994-11, Vol.223 (1), p.74-81
Hauptverfasser:	Affolter, M., Watts, J.D., Krebs, D.L., Aebersold, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chromatography, Thin Layer Mass Spectrometry Membrane Proteins - metabolism Molecular Sequence Data Phosphopeptides - analysis Phosphorylation Receptors, Antigen, T-Cell - metabolism
Online-Zugang:	Volltext
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