Rat mesencephalic neuronal cells cultured for different periods as a model of dopamine transporter ontogenesis
Ventral mesencephalic neurons contained only low-affinity and sodium-independent binding sites of [3H]WIN 35,428 (marker of dopamine transporter) during the first 10 d in primary cultures. These sites were present in cytosol, and they are not very probably related to dopamine transporter. After 12 d...
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| Veröffentlicht in: | Molecular neurobiology 1995-08, Vol.11 (1-3), p.111-119 |
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| container_title | Molecular neurobiology |
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| creator | Valchár, M Hanbauer, I |
| description | Ventral mesencephalic neurons contained only low-affinity and sodium-independent binding sites of [3H]WIN 35,428 (marker of dopamine transporter) during the first 10 d in primary cultures. These sites were present in cytosol, and they are not very probably related to dopamine transporter. After 12 d in culture, membrane-bound, high-affinity, and sodium-dependent [3H]WIN 35,428 binding sites were detected. In membranes prepared from cells 14 d in culture, cocaine displaced [3H]WIN 35,428 binding with similar potency to that in striatal membranes of adult rat brain. The high-affinity [3H]WIN 35,428 binding sites in mesencephalic neuronal cell cultures are very probably related to dopamine transporter. The development of high-affinity [3H]WIN 35,428 binding sites in neurons cultured for different time periods could be a useful model of dopamine transporter ontogenesis. |
| doi_str_mv | 10.1007/BF02740689 |
| format | Article |
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These sites were present in cytosol, and they are not very probably related to dopamine transporter. After 12 d in culture, membrane-bound, high-affinity, and sodium-dependent [3H]WIN 35,428 binding sites were detected. In membranes prepared from cells 14 d in culture, cocaine displaced [3H]WIN 35,428 binding with similar potency to that in striatal membranes of adult rat brain. The high-affinity [3H]WIN 35,428 binding sites in mesencephalic neuronal cell cultures are very probably related to dopamine transporter. The development of high-affinity [3H]WIN 35,428 binding sites in neurons cultured for different time periods could be a useful model of dopamine transporter ontogenesis.</description><identifier>ISSN: 0893-7648</identifier><identifier>EISSN: 1559-1182</identifier><identifier>DOI: 10.1007/BF02740689</identifier><identifier>PMID: 8561956</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Binding, Competitive ; Carrier Proteins - biosynthesis ; Carrier Proteins - drug effects ; Carrier Proteins - metabolism ; Cells, Cultured ; Cocaine - analogs & derivatives ; Cocaine - metabolism ; Cocaine - pharmacology ; Dopamine - metabolism ; Dopamine Plasma Membrane Transport Proteins ; Dopamine Uptake Inhibitors - pharmacology ; Embryo, Mammalian ; Embryonic and Fetal Development ; Female ; Gestational Age ; Kinetics ; Membrane Glycoproteins ; Membrane Transport Proteins ; Mesencephalon - cytology ; Mesencephalon - metabolism ; Models, Neurological ; Nerve Tissue Proteins ; Neurons - cytology ; Neurons - metabolism ; Piperazines - pharmacology ; Pregnancy ; Rats ; Rats, Sprague-Dawley ; Time Factors ; Tritium</subject><ispartof>Molecular neurobiology, 1995-08, Vol.11 (1-3), p.111-119</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c228t-bda6f474ae1b67c70bcb22507759891cefedcb3acee4b1468ec2d13e5d47e5423</citedby><cites>FETCH-LOGICAL-c228t-bda6f474ae1b67c70bcb22507759891cefedcb3acee4b1468ec2d13e5d47e5423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8561956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valchár, M</creatorcontrib><creatorcontrib>Hanbauer, I</creatorcontrib><title>Rat mesencephalic neuronal cells cultured for different periods as a model of dopamine transporter ontogenesis</title><title>Molecular neurobiology</title><addtitle>Mol Neurobiol</addtitle><description>Ventral mesencephalic neurons contained only low-affinity and sodium-independent binding sites of [3H]WIN 35,428 (marker of dopamine transporter) during the first 10 d in primary cultures. These sites were present in cytosol, and they are not very probably related to dopamine transporter. After 12 d in culture, membrane-bound, high-affinity, and sodium-dependent [3H]WIN 35,428 binding sites were detected. In membranes prepared from cells 14 d in culture, cocaine displaced [3H]WIN 35,428 binding with similar potency to that in striatal membranes of adult rat brain. The high-affinity [3H]WIN 35,428 binding sites in mesencephalic neuronal cell cultures are very probably related to dopamine transporter. The development of high-affinity [3H]WIN 35,428 binding sites in neurons cultured for different time periods could be a useful model of dopamine transporter ontogenesis.</description><subject>Animals</subject><subject>Binding, Competitive</subject><subject>Carrier Proteins - biosynthesis</subject><subject>Carrier Proteins - drug effects</subject><subject>Carrier Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Cocaine - analogs & derivatives</subject><subject>Cocaine - metabolism</subject><subject>Cocaine - pharmacology</subject><subject>Dopamine - metabolism</subject><subject>Dopamine Plasma Membrane Transport Proteins</subject><subject>Dopamine Uptake Inhibitors - pharmacology</subject><subject>Embryo, Mammalian</subject><subject>Embryonic and Fetal Development</subject><subject>Female</subject><subject>Gestational Age</subject><subject>Kinetics</subject><subject>Membrane Glycoproteins</subject><subject>Membrane Transport Proteins</subject><subject>Mesencephalon - cytology</subject><subject>Mesencephalon - metabolism</subject><subject>Models, Neurological</subject><subject>Nerve Tissue Proteins</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Piperazines - pharmacology</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Time Factors</subject><subject>Tritium</subject><issn>0893-7648</issn><issn>1559-1182</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cFLwzAUBvAgypzTi3chJw9CNUmTJj3qcCoMBNFzSZMXrbRJTdKD_70bG3oUHrzLjw_e-xA6p-SaEiJv7laESU4qVR-gORWiLihV7BDNiarLQlZcHaOTlD4JYYwSOUMzJSpai2qO_IvOeIAE3sD4ofvOYA9TDF732EDfJ2ymPk8RLHYhYts5BxF8xiPELtiE9WbwECz0ODhsw6iHzgPOUfs0hpgh4uBzeAcPqUun6MjpPsHZfi_Q2-r-dflYrJ8fnpa368IwpnLRWl05LrkG2lbSSNKaljFBpBS1qqkBB9a0pTYAvKW8UmCYpSUIyyUIzsoFutzljjF8TZByM3Rpe4_2EKbUSCkVE0z9C6kkhJel3MCrHTQxpBTBNWPsBh2_G0qabQvNXwsbfLFPndoB7C_dv738AegWhEs</recordid><startdate>199508</startdate><enddate>199508</enddate><creator>Valchár, M</creator><creator>Hanbauer, I</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199508</creationdate><title>Rat mesencephalic neuronal cells cultured for different periods as a model of dopamine transporter ontogenesis</title><author>Valchár, M ; Hanbauer, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c228t-bda6f474ae1b67c70bcb22507759891cefedcb3acee4b1468ec2d13e5d47e5423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Binding, Competitive</topic><topic>Carrier Proteins - biosynthesis</topic><topic>Carrier Proteins - drug effects</topic><topic>Carrier Proteins - metabolism</topic><topic>Cells, Cultured</topic><topic>Cocaine - analogs & derivatives</topic><topic>Cocaine - metabolism</topic><topic>Cocaine - pharmacology</topic><topic>Dopamine - metabolism</topic><topic>Dopamine Plasma Membrane Transport Proteins</topic><topic>Dopamine Uptake Inhibitors - pharmacology</topic><topic>Embryo, Mammalian</topic><topic>Embryonic and Fetal Development</topic><topic>Female</topic><topic>Gestational Age</topic><topic>Kinetics</topic><topic>Membrane Glycoproteins</topic><topic>Membrane Transport Proteins</topic><topic>Mesencephalon - cytology</topic><topic>Mesencephalon - metabolism</topic><topic>Models, Neurological</topic><topic>Nerve Tissue Proteins</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Piperazines - pharmacology</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Time Factors</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valchár, M</creatorcontrib><creatorcontrib>Hanbauer, I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valchár, M</au><au>Hanbauer, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rat mesencephalic neuronal cells cultured for different periods as a model of dopamine transporter ontogenesis</atitle><jtitle>Molecular neurobiology</jtitle><addtitle>Mol Neurobiol</addtitle><date>1995-08</date><risdate>1995</risdate><volume>11</volume><issue>1-3</issue><spage>111</spage><epage>119</epage><pages>111-119</pages><issn>0893-7648</issn><eissn>1559-1182</eissn><abstract>Ventral mesencephalic neurons contained only low-affinity and sodium-independent binding sites of [3H]WIN 35,428 (marker of dopamine transporter) during the first 10 d in primary cultures. These sites were present in cytosol, and they are not very probably related to dopamine transporter. After 12 d in culture, membrane-bound, high-affinity, and sodium-dependent [3H]WIN 35,428 binding sites were detected. In membranes prepared from cells 14 d in culture, cocaine displaced [3H]WIN 35,428 binding with similar potency to that in striatal membranes of adult rat brain. The high-affinity [3H]WIN 35,428 binding sites in mesencephalic neuronal cell cultures are very probably related to dopamine transporter. The development of high-affinity [3H]WIN 35,428 binding sites in neurons cultured for different time periods could be a useful model of dopamine transporter ontogenesis.</abstract><cop>United States</cop><pmid>8561956</pmid><doi>10.1007/BF02740689</doi><tpages>9</tpages></addata></record> |
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| ispartof | Molecular neurobiology, 1995-08, Vol.11 (1-3), p.111-119 |
| issn | 0893-7648 1559-1182 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Binding, Competitive Carrier Proteins - biosynthesis Carrier Proteins - drug effects Carrier Proteins - metabolism Cells, Cultured Cocaine - analogs & derivatives Cocaine - metabolism Cocaine - pharmacology Dopamine - metabolism Dopamine Plasma Membrane Transport Proteins Dopamine Uptake Inhibitors - pharmacology Embryo, Mammalian Embryonic and Fetal Development Female Gestational Age Kinetics Membrane Glycoproteins Membrane Transport Proteins Mesencephalon - cytology Mesencephalon - metabolism Models, Neurological Nerve Tissue Proteins Neurons - cytology Neurons - metabolism Piperazines - pharmacology Pregnancy Rats Rats, Sprague-Dawley Time Factors Tritium |
| title | Rat mesencephalic neuronal cells cultured for different periods as a model of dopamine transporter ontogenesis |
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