Mouse Cellular Nucleic Acid Binding Proteins: A Highly Conserved Family Identified by Genetic Mapping and Sequencing
Human cellular nucleic acid binding protein (CNBP) is a zinc finger DNA binding protein of unknown function. The human CNBP cDNA was used as a probe to isolate four structurally distinct but highly homologous mouse liver cDNA clones. Each of the mouse clones exhibited extraordinary sequence conserva...
Gespeichert in:
| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1994-11, Vol.24 (1), p.14-19 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 19 |
|---|---|
| container_issue | 1 |
| container_start_page | 14 |
| container_title | Genomics (San Diego, Calif.) |
| container_volume | 24 |
| creator | Warden, Craig H. Krisans, Skaidrite K. Purcell-Huynh, Deborah Leete, Lisa M. Daluiski, Aaron Diep, Anh Taylor, Benjamin A. Lusis, Aldons J. |
| description | Human cellular nucleic acid binding protein (CNBP) is a zinc finger DNA binding protein of unknown function. The human CNBP cDNA was used as a probe to isolate four structurally distinct but highly homologous mouse liver cDNA clones. Each of the mouse clones exhibited extraordinary sequence conservation with human CNBP cDNA, and the predicted mouse amino acid sequence identities with human CNBP protein ranged from 99 to 100%. Genetic mapping of CNBP genes in interspecific and intersubspecific mouse backcrosses revealed two loci that hybridize to CNBP cDNA at high stringency, located on chromosomes 5 and 6. The subcellular distribution of the CNBP protein was characterized with a specific polyclonal antibody generated against a synthetic peptide from the carboxyl terminus. CNBP was found in the cytosol and the endoplasmic reticulum in subcellular fractions from mouse liver, but was undetectable in nuclear fractions. These data suggest that CNBP is a member of a highly conserved family of cytosolic proteins that may be encoded by multiple dispersed genes. |
| doi_str_mv | 10.1006/geno.1994.1576 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77775006</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S088875438471576X</els_id><sourcerecordid>16845978</sourcerecordid><originalsourceid>FETCH-LOGICAL-c399t-d7814e4ef65a2d870626c703a4bf14dd29c91b5b57f3b9eee3e240e69d193ad93</originalsourceid><addsrcrecordid>eNqFkUtv3CAUhVHUKpkm3WZXiUWVnadgMI_uJqO8pKSN1HaNMFxPiTx4Cnak-ffFmlF2VdkgLh-He89B6JKSJSVEfNlAHJZUa76kjRQnaEGJ0pUSXLxDC6KUqmTD2Rn6kPMLIUQzVZ-iU6m0qIVeoPFpmDLgNfT91NuEv02uh-DwygWPr0P0IW7wcxpGCDF_xSt8Hza_-z1eDzFDegWPb-02lMKDhziGLpRKu8d3EGEsMk92t5sVbPT4B_yZILpyvEDvO9tn-Hjcz9Gv25uf6_vq8fvdw3r1WDmm9Vh5qSgHDp1obO2VJKVlJwmzvO0o977WTtO2aRvZsVYDAIOaExDaU82s1-wcXR10d2kof-fRbEN2ZVQboYxtZFlNMfG_IBWKN1qqAi4PoEtDzgk6s0tha9PeUGLmPMych5nzMHMe5cGno_LUbsG_4ccAyv3n473NzvZdssWh_IYxVjNRs4KpAwbFrtcAyWQXipngQwI3Gj-Ef3XwFzTdptw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16845978</pqid></control><display><type>article</type><title>Mouse Cellular Nucleic Acid Binding Proteins: A Highly Conserved Family Identified by Genetic Mapping and Sequencing</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Warden, Craig H. ; Krisans, Skaidrite K. ; Purcell-Huynh, Deborah ; Leete, Lisa M. ; Daluiski, Aaron ; Diep, Anh ; Taylor, Benjamin A. ; Lusis, Aldons J.</creator><creatorcontrib>Warden, Craig H. ; Krisans, Skaidrite K. ; Purcell-Huynh, Deborah ; Leete, Lisa M. ; Daluiski, Aaron ; Diep, Anh ; Taylor, Benjamin A. ; Lusis, Aldons J.</creatorcontrib><description>Human cellular nucleic acid binding protein (CNBP) is a zinc finger DNA binding protein of unknown function. The human CNBP cDNA was used as a probe to isolate four structurally distinct but highly homologous mouse liver cDNA clones. Each of the mouse clones exhibited extraordinary sequence conservation with human CNBP cDNA, and the predicted mouse amino acid sequence identities with human CNBP protein ranged from 99 to 100%. Genetic mapping of CNBP genes in interspecific and intersubspecific mouse backcrosses revealed two loci that hybridize to CNBP cDNA at high stringency, located on chromosomes 5 and 6. The subcellular distribution of the CNBP protein was characterized with a specific polyclonal antibody generated against a synthetic peptide from the carboxyl terminus. CNBP was found in the cytosol and the endoplasmic reticulum in subcellular fractions from mouse liver, but was undetectable in nuclear fractions. These data suggest that CNBP is a member of a highly conserved family of cytosolic proteins that may be encoded by multiple dispersed genes.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1994.1576</identifier><identifier>PMID: 7896269</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Chromosome Mapping ; DNA, Complementary ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Humans ; Mice ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; RNA-Binding Proteins ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; Subcellular Fractions - metabolism ; Zinc Fingers</subject><ispartof>Genomics (San Diego, Calif.), 1994-11, Vol.24 (1), p.14-19</ispartof><rights>1994 Academic Press</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-d7814e4ef65a2d870626c703a4bf14dd29c91b5b57f3b9eee3e240e69d193ad93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/geno.1994.1576$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3323623$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7896269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Warden, Craig H.</creatorcontrib><creatorcontrib>Krisans, Skaidrite K.</creatorcontrib><creatorcontrib>Purcell-Huynh, Deborah</creatorcontrib><creatorcontrib>Leete, Lisa M.</creatorcontrib><creatorcontrib>Daluiski, Aaron</creatorcontrib><creatorcontrib>Diep, Anh</creatorcontrib><creatorcontrib>Taylor, Benjamin A.</creatorcontrib><creatorcontrib>Lusis, Aldons J.</creatorcontrib><title>Mouse Cellular Nucleic Acid Binding Proteins: A Highly Conserved Family Identified by Genetic Mapping and Sequencing</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Human cellular nucleic acid binding protein (CNBP) is a zinc finger DNA binding protein of unknown function. The human CNBP cDNA was used as a probe to isolate four structurally distinct but highly homologous mouse liver cDNA clones. Each of the mouse clones exhibited extraordinary sequence conservation with human CNBP cDNA, and the predicted mouse amino acid sequence identities with human CNBP protein ranged from 99 to 100%. Genetic mapping of CNBP genes in interspecific and intersubspecific mouse backcrosses revealed two loci that hybridize to CNBP cDNA at high stringency, located on chromosomes 5 and 6. The subcellular distribution of the CNBP protein was characterized with a specific polyclonal antibody generated against a synthetic peptide from the carboxyl terminus. CNBP was found in the cytosol and the endoplasmic reticulum in subcellular fractions from mouse liver, but was undetectable in nuclear fractions. These data suggest that CNBP is a member of a highly conserved family of cytosolic proteins that may be encoded by multiple dispersed genes.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>DNA, Complementary</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>RNA-Binding Proteins</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Subcellular Fractions - metabolism</subject><subject>Zinc Fingers</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv3CAUhVHUKpkm3WZXiUWVnadgMI_uJqO8pKSN1HaNMFxPiTx4Cnak-ffFmlF2VdkgLh-He89B6JKSJSVEfNlAHJZUa76kjRQnaEGJ0pUSXLxDC6KUqmTD2Rn6kPMLIUQzVZ-iU6m0qIVeoPFpmDLgNfT91NuEv02uh-DwygWPr0P0IW7wcxpGCDF_xSt8Hza_-z1eDzFDegWPb-02lMKDhziGLpRKu8d3EGEsMk92t5sVbPT4B_yZILpyvEDvO9tn-Hjcz9Gv25uf6_vq8fvdw3r1WDmm9Vh5qSgHDp1obO2VJKVlJwmzvO0o977WTtO2aRvZsVYDAIOaExDaU82s1-wcXR10d2kof-fRbEN2ZVQboYxtZFlNMfG_IBWKN1qqAi4PoEtDzgk6s0tha9PeUGLmPMych5nzMHMe5cGno_LUbsG_4ccAyv3n473NzvZdssWh_IYxVjNRs4KpAwbFrtcAyWQXipngQwI3Gj-Ef3XwFzTdptw</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>Warden, Craig H.</creator><creator>Krisans, Skaidrite K.</creator><creator>Purcell-Huynh, Deborah</creator><creator>Leete, Lisa M.</creator><creator>Daluiski, Aaron</creator><creator>Diep, Anh</creator><creator>Taylor, Benjamin A.</creator><creator>Lusis, Aldons J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19941101</creationdate><title>Mouse Cellular Nucleic Acid Binding Proteins: A Highly Conserved Family Identified by Genetic Mapping and Sequencing</title><author>Warden, Craig H. ; Krisans, Skaidrite K. ; Purcell-Huynh, Deborah ; Leete, Lisa M. ; Daluiski, Aaron ; Diep, Anh ; Taylor, Benjamin A. ; Lusis, Aldons J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-d7814e4ef65a2d870626c703a4bf14dd29c91b5b57f3b9eee3e240e69d193ad93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>DNA, Complementary</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>RNA-Binding Proteins</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Subcellular Fractions - metabolism</topic><topic>Zinc Fingers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Warden, Craig H.</creatorcontrib><creatorcontrib>Krisans, Skaidrite K.</creatorcontrib><creatorcontrib>Purcell-Huynh, Deborah</creatorcontrib><creatorcontrib>Leete, Lisa M.</creatorcontrib><creatorcontrib>Daluiski, Aaron</creatorcontrib><creatorcontrib>Diep, Anh</creatorcontrib><creatorcontrib>Taylor, Benjamin A.</creatorcontrib><creatorcontrib>Lusis, Aldons J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Warden, Craig H.</au><au>Krisans, Skaidrite K.</au><au>Purcell-Huynh, Deborah</au><au>Leete, Lisa M.</au><au>Daluiski, Aaron</au><au>Diep, Anh</au><au>Taylor, Benjamin A.</au><au>Lusis, Aldons J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mouse Cellular Nucleic Acid Binding Proteins: A Highly Conserved Family Identified by Genetic Mapping and Sequencing</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>24</volume><issue>1</issue><spage>14</spage><epage>19</epage><pages>14-19</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Human cellular nucleic acid binding protein (CNBP) is a zinc finger DNA binding protein of unknown function. The human CNBP cDNA was used as a probe to isolate four structurally distinct but highly homologous mouse liver cDNA clones. Each of the mouse clones exhibited extraordinary sequence conservation with human CNBP cDNA, and the predicted mouse amino acid sequence identities with human CNBP protein ranged from 99 to 100%. Genetic mapping of CNBP genes in interspecific and intersubspecific mouse backcrosses revealed two loci that hybridize to CNBP cDNA at high stringency, located on chromosomes 5 and 6. The subcellular distribution of the CNBP protein was characterized with a specific polyclonal antibody generated against a synthetic peptide from the carboxyl terminus. CNBP was found in the cytosol and the endoplasmic reticulum in subcellular fractions from mouse liver, but was undetectable in nuclear fractions. These data suggest that CNBP is a member of a highly conserved family of cytosolic proteins that may be encoded by multiple dispersed genes.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>7896269</pmid><doi>10.1006/geno.1994.1576</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0888-7543 |
| ispartof | Genomics (San Diego, Calif.), 1994-11, Vol.24 (1), p.14-19 |
| issn | 0888-7543 1089-8646 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77775006 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Chromosome Mapping DNA, Complementary DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology Genes. Genome Humans Mice Mice, Inbred C57BL Mice, Inbred CBA Molecular and cellular biology Molecular genetics Molecular Sequence Data RNA-Binding Proteins Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Subcellular Fractions - metabolism Zinc Fingers |
| title | Mouse Cellular Nucleic Acid Binding Proteins: A Highly Conserved Family Identified by Genetic Mapping and Sequencing |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T18%3A08%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mouse%20Cellular%20Nucleic%20Acid%20Binding%20Proteins:%20A%20Highly%20Conserved%20Family%20Identified%20by%20Genetic%20Mapping%20and%20Sequencing&rft.jtitle=Genomics%20(San%20Diego,%20Calif.)&rft.au=Warden,%20Craig%20H.&rft.date=1994-11-01&rft.volume=24&rft.issue=1&rft.spage=14&rft.epage=19&rft.pages=14-19&rft.issn=0888-7543&rft.eissn=1089-8646&rft_id=info:doi/10.1006/geno.1994.1576&rft_dat=%3Cproquest_cross%3E16845978%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16845978&rft_id=info:pmid/7896269&rft_els_id=S088875438471576X&rfr_iscdi=true |