Fluorescein derivatization of fibrinogen for flow cytometric analysis of fibrinogen binding to platelets

Dog and human fibrinogen were derivatized with N‐hydroxysuccinimidofluorescein and utilized for flow cytometric estimation of fibrinogen binding to activated platelets. Fluorescein‐fibrinogen binding fulfilled the criteria for specific binding to platelets; the binding was saturable, dependent on ag...

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Veröffentlicht in:	Cytometry (New York, N.Y.) N.Y.), 1994-12, Vol.17 (4), p.287-293
Hauptverfasser:	Heilmann, Eric, Hynes, Laurie A., Burstein, Samuel A., George, James N., Dale, George L.
Format:	Artikel
Sprache:	eng
Schlagworte:	activated platelets Adenosine Diphosphate - pharmacology Animals Blood Platelets - metabolism Cellular Senescence - drug effects Dogs Edetic Acid - pharmacology Fibrinogen - metabolism Flow Cytometry Fluoresceins fluorescent tag Humans N‐hydroxysuccinimido‐fluorescein Platelet Activating Factor - pharmacology Platelet Activation - drug effects Protein labeling
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