A Multiple Assay for Vitamin D Metabolites without High-Performance Liquid Chromatography

We describe a multiple assay of the three main vitamin D metabolites, 25OHD, 24,25(OH) 2D, and 1,25(OH) 2D, in 0.5 ml of serum, which does not require high-performance liquid chromatography. The assay involves extracting the serum with acetonitrile, separation and purification on a C-18/OH cartridge...

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Veröffentlicht in:	Analytical biochemistry 1994-11, Vol.222 (2), p.359-365
Hauptverfasser:	Wei, S., Tanaka, H., Kubo, T., Ichikawa, M., Seino, Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitriles Animals Child Chromatography, Gel - methods Chromatography, High Pressure Liquid Humans Hydroxycholecalciferols - blood Rats Reproducibility of Results
Online-Zugang:	Volltext
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