Interaction of cationic liposomes with cells of electrically active neuronal networks in culture
Incubation of rhodamine-labeled cationic liposomes with mature murine spinal cultures results in strong fluorescence that is evenly distributed on somata and neurites of neurons in 7 different cultures. Staining of the glial carpet is minimal. Rhodamine-labeled dextran, excapsulated in liposomes, al...
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| Veröffentlicht in: | Brain research 1995-10, Vol.695 (2), p.231-236 |
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| creator | Azzazy, Hassan M.E. Hong, Keelung Wu, Ming-Chi Gross, Guenter W. |
| description | Incubation of rhodamine-labeled cationic liposomes with mature murine spinal cultures results in strong fluorescence that is evenly distributed on somata and neurites of neurons in 7 different cultures. Staining of the glial carpet is minimal. Rhodamine-labeled dextran, excapsulated in liposomes, also stains neurons. Electron microscope data show external attachment and intact internalization of liposomes. Spontaneous electrical bursting activity is altered but not lost after incubation. |
| doi_str_mv | 10.1016/0006-8993(95)00710-8 |
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Staining of the glial carpet is minimal. Rhodamine-labeled dextran, excapsulated in liposomes, also stains neurons. Electron microscope data show external attachment and intact internalization of liposomes. Spontaneous electrical bursting activity is altered but not lost after incubation.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(95)00710-8</identifier><identifier>PMID: 8556335</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cations - administration & dosage ; Cations - pharmacology ; Cell culture ; Cell Membrane - drug effects ; Cell Membrane - ultrastructure ; Cells, Cultured ; Electrophysiology ; Endocytosis - drug effects ; Endocytosis - physiology ; Extracellular Space - drug effects ; Extracellular Space - physiology ; Fundamental and applied biological sciences. 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All rights reserved</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-134782ac0615f39f3a554ec8338448089c6e8d794bd65d4ee62107c2065622793</citedby><cites>FETCH-LOGICAL-c417t-134782ac0615f39f3a554ec8338448089c6e8d794bd65d4ee62107c2065622793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-8993(95)00710-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3687297$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8556335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Azzazy, Hassan M.E.</creatorcontrib><creatorcontrib>Hong, Keelung</creatorcontrib><creatorcontrib>Wu, Ming-Chi</creatorcontrib><creatorcontrib>Gross, Guenter W.</creatorcontrib><title>Interaction of cationic liposomes with cells of electrically active neuronal networks in culture</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Incubation of rhodamine-labeled cationic liposomes with mature murine spinal cultures results in strong fluorescence that is evenly distributed on somata and neurites of neurons in 7 different cultures. Staining of the glial carpet is minimal. Rhodamine-labeled dextran, excapsulated in liposomes, also stains neurons. Electron microscope data show external attachment and intact internalization of liposomes. Spontaneous electrical bursting activity is altered but not lost after incubation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cations - administration & dosage</subject><subject>Cations - pharmacology</subject><subject>Cell culture</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cells, Cultured</subject><subject>Electrophysiology</subject><subject>Endocytosis - drug effects</subject><subject>Endocytosis - physiology</subject><subject>Extracellular Space - drug effects</subject><subject>Extracellular Space - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>Liposome endocytosis</subject><subject>Liposomes</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning Transmission</subject><subject>Multielectrode array</subject><subject>Nerve Net - cytology</subject><subject>Nerve Net - drug effects</subject><subject>Nerve Net - physiology</subject><subject>Neuronal network</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Neurons - ultrastructure</subject><subject>Rhodamines</subject><subject>Spinal Cord - cytology</subject><subject>Spinal Cord - drug effects</subject><subject>Spinal Cord - physiology</subject><subject>Staining and Labeling</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rVDEUxYNY6rT6HyhkIdIunub7Y1OQorZQ6EbXMc27D6OZlzF5b8r89-Y5wyzbVW64v3M5nIPQW0o-UkLVJ0KI6oy1_MLKS0I0JZ15gVbUaNYpJshLtDoir9BZrb_bl3NLTtGpkVJxLlfo5-04QfFhinnEecDBL1MMOMVNrnkNFT_G6RcOkFJdAEgQphKDT2mHF90W8AhzyaNPbZgec_lTcRxxmNM0F3iNTgafKrw5vOfox9cv369vurv7b7fXn--6IKieOsqFNswHoqgcuB24l1JAMJwbIQwxNigwvbbioVeyFwCKUaIDI0oqxrTl5-jD_u6m5L8z1MmtY11c-xHyXJ3WWilm9LMgVdYYyUgDxR4MJddaYHCbEte-7BwlbmnALfG6JV5npfvfgDNN9u5wf35YQ38UHSJv-_eHva8txaH4McR6xLhq_dnF5tUegxbaNkJxNUQYA_SxtAZcn-PTPv4BbFGhjg</recordid><startdate>19951016</startdate><enddate>19951016</enddate><creator>Azzazy, Hassan M.E.</creator><creator>Hong, Keelung</creator><creator>Wu, Ming-Chi</creator><creator>Gross, Guenter W.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19951016</creationdate><title>Interaction of cationic liposomes with cells of electrically active neuronal networks in culture</title><author>Azzazy, Hassan M.E. ; Hong, Keelung ; Wu, Ming-Chi ; Gross, Guenter W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-134782ac0615f39f3a554ec8338448089c6e8d794bd65d4ee62107c2065622793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cations - administration & dosage</topic><topic>Cations - pharmacology</topic><topic>Cell culture</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cells, Cultured</topic><topic>Electrophysiology</topic><topic>Endocytosis - drug effects</topic><topic>Endocytosis - physiology</topic><topic>Extracellular Space - drug effects</topic><topic>Extracellular Space - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Liposome endocytosis</topic><topic>Liposomes</topic><topic>Mice</topic><topic>Microscopy, Electron, Scanning Transmission</topic><topic>Multielectrode array</topic><topic>Nerve Net - cytology</topic><topic>Nerve Net - drug effects</topic><topic>Nerve Net - physiology</topic><topic>Neuronal network</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Neurons - ultrastructure</topic><topic>Rhodamines</topic><topic>Spinal Cord - cytology</topic><topic>Spinal Cord - drug effects</topic><topic>Spinal Cord - physiology</topic><topic>Staining and Labeling</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azzazy, Hassan M.E.</creatorcontrib><creatorcontrib>Hong, Keelung</creatorcontrib><creatorcontrib>Wu, Ming-Chi</creatorcontrib><creatorcontrib>Gross, Guenter W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azzazy, Hassan M.E.</au><au>Hong, Keelung</au><au>Wu, Ming-Chi</au><au>Gross, Guenter W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of cationic liposomes with cells of electrically active neuronal networks in culture</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1995-10-16</date><risdate>1995</risdate><volume>695</volume><issue>2</issue><spage>231</spage><epage>236</epage><pages>231-236</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Incubation of rhodamine-labeled cationic liposomes with mature murine spinal cultures results in strong fluorescence that is evenly distributed on somata and neurites of neurons in 7 different cultures. Staining of the glial carpet is minimal. Rhodamine-labeled dextran, excapsulated in liposomes, also stains neurons. Electron microscope data show external attachment and intact internalization of liposomes. Spontaneous electrical bursting activity is altered but not lost after incubation.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8556335</pmid><doi>10.1016/0006-8993(95)00710-8</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Cations - administration & dosage Cations - pharmacology Cell culture Cell Membrane - drug effects Cell Membrane - ultrastructure Cells, Cultured Electrophysiology Endocytosis - drug effects Endocytosis - physiology Extracellular Space - drug effects Extracellular Space - physiology Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Liposome endocytosis Liposomes Mice Microscopy, Electron, Scanning Transmission Multielectrode array Nerve Net - cytology Nerve Net - drug effects Nerve Net - physiology Neuronal network Neurons - drug effects Neurons - physiology Neurons - ultrastructure Rhodamines Spinal Cord - cytology Spinal Cord - drug effects Spinal Cord - physiology Staining and Labeling Vertebrates: nervous system and sense organs |
| title | Interaction of cationic liposomes with cells of electrically active neuronal networks in culture |
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