Purification and Characterization of Rat Parotid Glycosylated, Basic and Acidic Proline-Rich Proteins
A unique family of proline-rich proteins (PRPs) is induced in rats following prolonged isoproterenol treatment. PRPs can be divided into glycosylated (GPRP), basic (BPRP) and acidic (APRP) proline-rich proteins based on their physicochemical characteristics. Inducible rat parotid PRPs were isolated...
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| Veröffentlicht in: | Preparative biochemistry 1995-08, Vol.25 (3), p.119-132 |
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| creator | Bedi, Gurrinder S. Bedi, Surinder K. |
| description | A unique family of proline-rich proteins (PRPs) is induced in rats following prolonged isoproterenol treatment. PRPs can be divided into glycosylated (GPRP), basic (BPRP) and acidic (APRP) proline-rich proteins based on their physicochemical characteristics. Inducible rat parotid PRPs were isolated from aqueous extracts of parotid glands of isoproterenol-treated animals by sequential chromatography on columns of DEAE-Sepharose CL-6B, Sephadex G-100 and FPLC on Suprose-12 column. The GPRP showed a single homogeneous band on sodium dodecylpolyacrylamide gel electrophoresis with an estimated molecular weight of approximately 220,000. Compositional analysis of GPRP revealed that this protein contained 19.7% glutamic acid/glutamine, 28.2% proline and 9.5% glycine, and 44% carbohydrate, consisting of fucose (2.81g/100g), mannose (9.78g/100g), galactose (9.29g/100g), N-acetylglucosamine (18.03g/100g) and N-acetylgalactosamine (3.90g/100g). Basic PRPs consisted of a family of proteins with estimated molecular masses ranging from 14-45 kDa. These proteins contained 42.6% proline, 20.65% glutamic acid/glutamine and 21.33% glycine. Acidic PRPs also comprised of a family of metachromatically stained ladder of 40-60 kDa containing 29.1% proline, 21.5% glutamic acid/glutamine and 17.8% glycine. APRP were heavily glycosylated containing N-acetylglucosamine (6.34g/100g), N-acetylgalactosamine (19.04g/100g) and glucuronic acid (38.08g/100g). |
| doi_str_mv | 10.1080/10826069508010115 |
| format | Article |
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PRPs can be divided into glycosylated (GPRP), basic (BPRP) and acidic (APRP) proline-rich proteins based on their physicochemical characteristics. Inducible rat parotid PRPs were isolated from aqueous extracts of parotid glands of isoproterenol-treated animals by sequential chromatography on columns of DEAE-Sepharose CL-6B, Sephadex G-100 and FPLC on Suprose-12 column. The GPRP showed a single homogeneous band on sodium dodecylpolyacrylamide gel electrophoresis with an estimated molecular weight of approximately 220,000. Compositional analysis of GPRP revealed that this protein contained 19.7% glutamic acid/glutamine, 28.2% proline and 9.5% glycine, and 44% carbohydrate, consisting of fucose (2.81g/100g), mannose (9.78g/100g), galactose (9.29g/100g), N-acetylglucosamine (18.03g/100g) and N-acetylgalactosamine (3.90g/100g). Basic PRPs consisted of a family of proteins with estimated molecular masses ranging from 14-45 kDa. These proteins contained 42.6% proline, 20.65% glutamic acid/glutamine and 21.33% glycine. Acidic PRPs also comprised of a family of metachromatically stained ladder of 40-60 kDa containing 29.1% proline, 21.5% glutamic acid/glutamine and 17.8% glycine. APRP were heavily glycosylated containing N-acetylglucosamine (6.34g/100g), N-acetylgalactosamine (19.04g/100g) and glucuronic acid (38.08g/100g).</description><identifier>ISSN: 0032-7484</identifier><identifier>DOI: 10.1080/10826069508010115</identifier><identifier>PMID: 8532636</identifier><language>eng</language><publisher>United States: Taylor & Francis Group</publisher><subject>Amino Acids - analysis ; Animals ; Carbohydrates - analysis ; Chromatography, Ion Exchange ; Electrophoresis, Polyacrylamide Gel ; Glycosylation ; Hydrogen-Ion Concentration ; Male ; Parotid Gland - chemistry ; Peptides - chemistry ; Peptides - isolation & purification ; Proline-Rich Protein Domains ; Rats ; Rats, Wistar ; Salivary Proteins and Peptides - chemistry ; Salivary Proteins and Peptides - isolation & purification</subject><ispartof>Preparative biochemistry, 1995-08, Vol.25 (3), p.119-132</ispartof><rights>Copyright Taylor & Francis Group, LLC 1995</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c264t-e8cea6a458a804c17e998a24acaf3720a6148366808beed20df266a69768a15b3</citedby><cites>FETCH-LOGICAL-c264t-e8cea6a458a804c17e998a24acaf3720a6148366808beed20df266a69768a15b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/10826069508010115$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/10826069508010115$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,59623,60412</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8532636$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bedi, Gurrinder S.</creatorcontrib><creatorcontrib>Bedi, Surinder K.</creatorcontrib><title>Purification and Characterization of Rat Parotid Glycosylated, Basic and Acidic Proline-Rich Proteins</title><title>Preparative biochemistry</title><addtitle>Prep Biochem</addtitle><description>A unique family of proline-rich proteins (PRPs) is induced in rats following prolonged isoproterenol treatment. PRPs can be divided into glycosylated (GPRP), basic (BPRP) and acidic (APRP) proline-rich proteins based on their physicochemical characteristics. Inducible rat parotid PRPs were isolated from aqueous extracts of parotid glands of isoproterenol-treated animals by sequential chromatography on columns of DEAE-Sepharose CL-6B, Sephadex G-100 and FPLC on Suprose-12 column. The GPRP showed a single homogeneous band on sodium dodecylpolyacrylamide gel electrophoresis with an estimated molecular weight of approximately 220,000. Compositional analysis of GPRP revealed that this protein contained 19.7% glutamic acid/glutamine, 28.2% proline and 9.5% glycine, and 44% carbohydrate, consisting of fucose (2.81g/100g), mannose (9.78g/100g), galactose (9.29g/100g), N-acetylglucosamine (18.03g/100g) and N-acetylgalactosamine (3.90g/100g). Basic PRPs consisted of a family of proteins with estimated molecular masses ranging from 14-45 kDa. These proteins contained 42.6% proline, 20.65% glutamic acid/glutamine and 21.33% glycine. Acidic PRPs also comprised of a family of metachromatically stained ladder of 40-60 kDa containing 29.1% proline, 21.5% glutamic acid/glutamine and 17.8% glycine. APRP were heavily glycosylated containing N-acetylglucosamine (6.34g/100g), N-acetylgalactosamine (19.04g/100g) and glucuronic acid (38.08g/100g).</description><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Carbohydrates - analysis</subject><subject>Chromatography, Ion Exchange</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Glycosylation</subject><subject>Hydrogen-Ion Concentration</subject><subject>Male</subject><subject>Parotid Gland - chemistry</subject><subject>Peptides - chemistry</subject><subject>Peptides - isolation & purification</subject><subject>Proline-Rich Protein Domains</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Salivary Proteins and Peptides - chemistry</subject><subject>Salivary Proteins and Peptides - isolation & purification</subject><issn>0032-7484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UD1PwzAU9AAqpfADGJAyMRGwncRxJJZSQUGqRFXBHL36QzVy4mK7QuHXk5KKBbG8e_fu3Q2H0AXBNwRzfNsPyjCrip4QTEhxhMYYZzQtc56foNMQ3ntKioyO0Ij3wDI2Rmq580YbAdG4NoFWJrMNeBBRefM1HJ1OVhCTJXgXjUzmthMudBaiktfJPQQjfnxTYWS_Lr2zplXpyojNnkRl2nCGjjXYoM4POEFvjw-vs6d08TJ_nk0XqaAsj6niQgGDvODAcS5IqaqKA81BgM5KioGRnGeMcczXSkmKpaaMAatKxoEU62yCrobcrXcfOxVi3ZgglLXQKrcLdVmWBS0o7h_J8Ci8C8ErXW-9acB3NcH1vs76T5295_IQvls3Sv46Dl32-t2gm1Y738Cn81bWETrrvPbQChPq7P_4by4FhJM</recordid><startdate>199508</startdate><enddate>199508</enddate><creator>Bedi, Gurrinder S.</creator><creator>Bedi, Surinder K.</creator><general>Taylor & Francis Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199508</creationdate><title>Purification and Characterization of Rat Parotid Glycosylated, Basic and Acidic Proline-Rich Proteins</title><author>Bedi, Gurrinder S. ; Bedi, Surinder K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-e8cea6a458a804c17e998a24acaf3720a6148366808beed20df266a69768a15b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Carbohydrates - analysis</topic><topic>Chromatography, Ion Exchange</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Glycosylation</topic><topic>Hydrogen-Ion Concentration</topic><topic>Male</topic><topic>Parotid Gland - chemistry</topic><topic>Peptides - chemistry</topic><topic>Peptides - isolation & purification</topic><topic>Proline-Rich Protein Domains</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Salivary Proteins and Peptides - chemistry</topic><topic>Salivary Proteins and Peptides - isolation & purification</topic><toplevel>online_resources</toplevel><creatorcontrib>Bedi, Gurrinder S.</creatorcontrib><creatorcontrib>Bedi, Surinder K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Preparative biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bedi, Gurrinder S.</au><au>Bedi, Surinder K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and Characterization of Rat Parotid Glycosylated, Basic and Acidic Proline-Rich Proteins</atitle><jtitle>Preparative biochemistry</jtitle><addtitle>Prep Biochem</addtitle><date>1995-08</date><risdate>1995</risdate><volume>25</volume><issue>3</issue><spage>119</spage><epage>132</epage><pages>119-132</pages><issn>0032-7484</issn><abstract>A unique family of proline-rich proteins (PRPs) is induced in rats following prolonged isoproterenol treatment. PRPs can be divided into glycosylated (GPRP), basic (BPRP) and acidic (APRP) proline-rich proteins based on their physicochemical characteristics. Inducible rat parotid PRPs were isolated from aqueous extracts of parotid glands of isoproterenol-treated animals by sequential chromatography on columns of DEAE-Sepharose CL-6B, Sephadex G-100 and FPLC on Suprose-12 column. The GPRP showed a single homogeneous band on sodium dodecylpolyacrylamide gel electrophoresis with an estimated molecular weight of approximately 220,000. Compositional analysis of GPRP revealed that this protein contained 19.7% glutamic acid/glutamine, 28.2% proline and 9.5% glycine, and 44% carbohydrate, consisting of fucose (2.81g/100g), mannose (9.78g/100g), galactose (9.29g/100g), N-acetylglucosamine (18.03g/100g) and N-acetylgalactosamine (3.90g/100g). Basic PRPs consisted of a family of proteins with estimated molecular masses ranging from 14-45 kDa. These proteins contained 42.6% proline, 20.65% glutamic acid/glutamine and 21.33% glycine. Acidic PRPs also comprised of a family of metachromatically stained ladder of 40-60 kDa containing 29.1% proline, 21.5% glutamic acid/glutamine and 17.8% glycine. APRP were heavily glycosylated containing N-acetylglucosamine (6.34g/100g), N-acetylgalactosamine (19.04g/100g) and glucuronic acid (38.08g/100g).</abstract><cop>United States</cop><pub>Taylor & Francis Group</pub><pmid>8532636</pmid><doi>10.1080/10826069508010115</doi><tpages>14</tpages></addata></record> |
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| subjects | Amino Acids - analysis Animals Carbohydrates - analysis Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Glycosylation Hydrogen-Ion Concentration Male Parotid Gland - chemistry Peptides - chemistry Peptides - isolation & purification Proline-Rich Protein Domains Rats Rats, Wistar Salivary Proteins and Peptides - chemistry Salivary Proteins and Peptides - isolation & purification |
| title | Purification and Characterization of Rat Parotid Glycosylated, Basic and Acidic Proline-Rich Proteins |
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