Increased bone formation by intermittent parathyroid hormone administration is due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow
In order to examine the mechanism of the anabolic effect of parathyroid hormone (PTH) on bone formation, human PTH(1-34) [hPTH(1-34)] (30 μg/kg) was injected subcutaneously to 9-week-old rats 5 times a week for 1 or 3 weeks. Trabecular bone volume (BV/TV) in the tibial metaphysis was not significant...
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| Veröffentlicht in: | Bone (New York, N.Y.) N.Y.), 1994-11, Vol.15 (6), p.717-723 |
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| creator | Nishida, S. Yamaguchi, A. Tanizawa, T. Endo, N. Mashiba, T. Uchiyama, Y. Suda, T. Yoshiki, S. Takahashi, H.E. |
| description | In order to examine the mechanism of the anabolic effect of parathyroid hormone (PTH) on bone formation, human PTH(1-34) [hPTH(1-34)] (30 μg/kg) was injected subcutaneously to 9-week-old rats 5 times a week for 1 or 3 weeks. Trabecular bone volume (BV/TV) in the tibial metaphysis was not significantly different between the PTH- and vehicletreated groups, but the parameters related to bone formation, including osteoid surface (OS/BS), mineralizing surface (MS/BS), mineral apposition rate (MAR), and bone formation rate (BFR/BS), were significantly increased as early as 1 week after PTH treatment. And the parameters related to bone resorption including eroded surface (ES/BS) and osteoclast number (N.Oc/BS) were also significantly increased as early as 1 week after PTH treatment. Treatment with PTH for 1 week induced no significant increase in bone mineral density at the femoral metaphysis, whereas the same treatment for 3 weeks induced a significant increase. When bone marrow cells isolated from femora and tibiae of either PTH- or vehicle-treated rats were cultured at a high density (2 × 10
7 cells/one well of 24-multiwell plate), cellular alkaline phosphatase (ALP) activity was significantly increased in the cells isolated from PTH-treated rats compared with vehicletreated rats. When bone marrow cells were cultured at a low density (4 × 10
6 cells/a one well of 6-multiwell plate) to generate colonies (colony forming unit-fibroblastic, CFU-F), PTH induced apparent increases in both the total number of CFU-F and the number of ALP-positive CFU-F. The ratio of the latter to the former was significantly higher in the PTH-treated group than in the vehicle-treated group. These findings suggest that the anabolic effect of PTH is, at least in part, due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow. |
| doi_str_mv | 10.1016/8756-3282(94)90322-0 |
| format | Article |
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7 cells/one well of 24-multiwell plate), cellular alkaline phosphatase (ALP) activity was significantly increased in the cells isolated from PTH-treated rats compared with vehicletreated rats. When bone marrow cells were cultured at a low density (4 × 10
6 cells/a one well of 6-multiwell plate) to generate colonies (colony forming unit-fibroblastic, CFU-F), PTH induced apparent increases in both the total number of CFU-F and the number of ALP-positive CFU-F. The ratio of the latter to the former was significantly higher in the PTH-treated group than in the vehicle-treated group. These findings suggest that the anabolic effect of PTH is, at least in part, due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow.</description><identifier>ISSN: 8756-3282</identifier><identifier>EISSN: 1873-2763</identifier><identifier>DOI: 10.1016/8756-3282(94)90322-0</identifier><identifier>PMID: 7873302</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Alkaline Phosphatase - metabolism ; Animals ; Biological and medical sciences ; Biomechanical Phenomena ; Bone Density - drug effects ; Bone Development - drug effects ; Bone Marrow - drug effects ; Bone Marrow Cells ; Bone marrow stromal cell ; Cell Count ; Cell Differentiation - drug effects ; Cell Division - drug effects ; Cells, Cultured ; CFU-F ; Colony-Forming Units Assay ; Female ; Femur - drug effects ; Fundamental and applied biological sciences. Psychology ; Hormones. Regulation ; Humans ; Injections, Subcutaneous ; Osteoclasts - drug effects ; Osteoprogenitor cells ; Parathyroid hormone ; Parathyroid Hormone - administration & dosage ; Parathyroid Hormone - pharmacology ; Peptide Fragments - administration & dosage ; Peptide Fragments - pharmacology ; Rats ; Rats, Sprague-Dawley ; Stem Cells - cytology ; Stem Cells - drug effects ; Teriparatide ; Thyroid. Parathyroid. Ultimobranchial body ; Tibia - drug effects ; Vertebrates: endocrinology</subject><ispartof>Bone (New York, N.Y.), 1994-11, Vol.15 (6), p.717-723</ispartof><rights>1994</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-b17571ed67e0613b287e1b24e5e573776ff6abc3916217af811d6d8f3251eb0e3</citedby><cites>FETCH-LOGICAL-c452t-b17571ed67e0613b287e1b24e5e573776ff6abc3916217af811d6d8f3251eb0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/8756328294903220$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3536,23910,23911,25119,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3304992$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7873302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishida, S.</creatorcontrib><creatorcontrib>Yamaguchi, A.</creatorcontrib><creatorcontrib>Tanizawa, T.</creatorcontrib><creatorcontrib>Endo, N.</creatorcontrib><creatorcontrib>Mashiba, T.</creatorcontrib><creatorcontrib>Uchiyama, Y.</creatorcontrib><creatorcontrib>Suda, T.</creatorcontrib><creatorcontrib>Yoshiki, S.</creatorcontrib><creatorcontrib>Takahashi, H.E.</creatorcontrib><title>Increased bone formation by intermittent parathyroid hormone administration is due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow</title><title>Bone (New York, N.Y.)</title><addtitle>Bone</addtitle><description>In order to examine the mechanism of the anabolic effect of parathyroid hormone (PTH) on bone formation, human PTH(1-34) [hPTH(1-34)] (30 μg/kg) was injected subcutaneously to 9-week-old rats 5 times a week for 1 or 3 weeks. Trabecular bone volume (BV/TV) in the tibial metaphysis was not significantly different between the PTH- and vehicletreated groups, but the parameters related to bone formation, including osteoid surface (OS/BS), mineralizing surface (MS/BS), mineral apposition rate (MAR), and bone formation rate (BFR/BS), were significantly increased as early as 1 week after PTH treatment. And the parameters related to bone resorption including eroded surface (ES/BS) and osteoclast number (N.Oc/BS) were also significantly increased as early as 1 week after PTH treatment. Treatment with PTH for 1 week induced no significant increase in bone mineral density at the femoral metaphysis, whereas the same treatment for 3 weeks induced a significant increase. When bone marrow cells isolated from femora and tibiae of either PTH- or vehicle-treated rats were cultured at a high density (2 × 10
7 cells/one well of 24-multiwell plate), cellular alkaline phosphatase (ALP) activity was significantly increased in the cells isolated from PTH-treated rats compared with vehicletreated rats. When bone marrow cells were cultured at a low density (4 × 10
6 cells/a one well of 6-multiwell plate) to generate colonies (colony forming unit-fibroblastic, CFU-F), PTH induced apparent increases in both the total number of CFU-F and the number of ALP-positive CFU-F. The ratio of the latter to the former was significantly higher in the PTH-treated group than in the vehicle-treated group. These findings suggest that the anabolic effect of PTH is, at least in part, due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomechanical Phenomena</subject><subject>Bone Density - drug effects</subject><subject>Bone Development - drug effects</subject><subject>Bone Marrow - drug effects</subject><subject>Bone Marrow Cells</subject><subject>Bone marrow stromal cell</subject><subject>Cell Count</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>CFU-F</subject><subject>Colony-Forming Units Assay</subject><subject>Female</subject><subject>Femur - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones. Regulation</subject><subject>Humans</subject><subject>Injections, Subcutaneous</subject><subject>Osteoclasts - drug effects</subject><subject>Osteoprogenitor cells</subject><subject>Parathyroid hormone</subject><subject>Parathyroid Hormone - administration & dosage</subject><subject>Parathyroid Hormone - pharmacology</subject><subject>Peptide Fragments - administration & dosage</subject><subject>Peptide Fragments - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Teriparatide</subject><subject>Thyroid. Parathyroid. Ultimobranchial body</subject><subject>Tibia - drug effects</subject><subject>Vertebrates: endocrinology</subject><issn>8756-3282</issn><issn>1873-2763</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90U2L1TAUBuAgyngd_QcKWYjoopqPNmk3AzL4MTDgRtchTU68kTa5Jqly_5a_0NSWu3RVynnO4SUvQs8peUsJFe962YmGs569Hto3A-GMNeQBOtBe8oZJwR-iw4U8Rk9y_kEI4YOkV-hKVsQJO6A_d8Ek0BksHmMA7GKadfEx4PGMfSiQZl8KhIJPOulyPKfoLT5WtWptZx98Lmlb8RnbBXCJuBwB5-LnZdom0eFTipN3sFMdLLbe1f96219QzAVild8h-BITNjBNuebYws06pfj7KXrk9JTh2f69Rt8-fvh6-7m5__Lp7vb9fWPajpVmpLKTFKyQQATlI-sl0JG10EEnuZTCOaFHwwcqGJXa9ZRaYXvHWUdhJMCv0avtbs3zc4Fc1OzzGkgHiEtWUspWtpRU2G7QpJhzAqdOydesZ0WJWqtSaw9q7UENrfpXlVrXXuz3l3EGe1nau6nzl_tcZ6Mnl3QwPl9YNe0wrOxmY1Df4peHpLLxEAxYn8AUZaP_f46_oxC1LA</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>Nishida, S.</creator><creator>Yamaguchi, A.</creator><creator>Tanizawa, T.</creator><creator>Endo, N.</creator><creator>Mashiba, T.</creator><creator>Uchiyama, Y.</creator><creator>Suda, T.</creator><creator>Yoshiki, S.</creator><creator>Takahashi, H.E.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941101</creationdate><title>Increased bone formation by intermittent parathyroid hormone administration is due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow</title><author>Nishida, S. ; Yamaguchi, A. ; Tanizawa, T. ; Endo, N. ; Mashiba, T. ; Uchiyama, Y. ; Suda, T. ; Yoshiki, S. ; Takahashi, H.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-b17571ed67e0613b287e1b24e5e573776ff6abc3916217af811d6d8f3251eb0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomechanical Phenomena</topic><topic>Bone Density - drug effects</topic><topic>Bone Development - drug effects</topic><topic>Bone Marrow - drug effects</topic><topic>Bone Marrow Cells</topic><topic>Bone marrow stromal cell</topic><topic>Cell Count</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>CFU-F</topic><topic>Colony-Forming Units Assay</topic><topic>Female</topic><topic>Femur - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones. Regulation</topic><topic>Humans</topic><topic>Injections, Subcutaneous</topic><topic>Osteoclasts - drug effects</topic><topic>Osteoprogenitor cells</topic><topic>Parathyroid hormone</topic><topic>Parathyroid Hormone - administration & dosage</topic><topic>Parathyroid Hormone - pharmacology</topic><topic>Peptide Fragments - administration & dosage</topic><topic>Peptide Fragments - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Teriparatide</topic><topic>Thyroid. Parathyroid. Ultimobranchial body</topic><topic>Tibia - drug effects</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishida, S.</creatorcontrib><creatorcontrib>Yamaguchi, A.</creatorcontrib><creatorcontrib>Tanizawa, T.</creatorcontrib><creatorcontrib>Endo, N.</creatorcontrib><creatorcontrib>Mashiba, T.</creatorcontrib><creatorcontrib>Uchiyama, Y.</creatorcontrib><creatorcontrib>Suda, T.</creatorcontrib><creatorcontrib>Yoshiki, S.</creatorcontrib><creatorcontrib>Takahashi, H.E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bone (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishida, S.</au><au>Yamaguchi, A.</au><au>Tanizawa, T.</au><au>Endo, N.</au><au>Mashiba, T.</au><au>Uchiyama, Y.</au><au>Suda, T.</au><au>Yoshiki, S.</au><au>Takahashi, H.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased bone formation by intermittent parathyroid hormone administration is due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow</atitle><jtitle>Bone (New York, N.Y.)</jtitle><addtitle>Bone</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>15</volume><issue>6</issue><spage>717</spage><epage>723</epage><pages>717-723</pages><issn>8756-3282</issn><eissn>1873-2763</eissn><abstract>In order to examine the mechanism of the anabolic effect of parathyroid hormone (PTH) on bone formation, human PTH(1-34) [hPTH(1-34)] (30 μg/kg) was injected subcutaneously to 9-week-old rats 5 times a week for 1 or 3 weeks. Trabecular bone volume (BV/TV) in the tibial metaphysis was not significantly different between the PTH- and vehicletreated groups, but the parameters related to bone formation, including osteoid surface (OS/BS), mineralizing surface (MS/BS), mineral apposition rate (MAR), and bone formation rate (BFR/BS), were significantly increased as early as 1 week after PTH treatment. And the parameters related to bone resorption including eroded surface (ES/BS) and osteoclast number (N.Oc/BS) were also significantly increased as early as 1 week after PTH treatment. Treatment with PTH for 1 week induced no significant increase in bone mineral density at the femoral metaphysis, whereas the same treatment for 3 weeks induced a significant increase. When bone marrow cells isolated from femora and tibiae of either PTH- or vehicle-treated rats were cultured at a high density (2 × 10
7 cells/one well of 24-multiwell plate), cellular alkaline phosphatase (ALP) activity was significantly increased in the cells isolated from PTH-treated rats compared with vehicletreated rats. When bone marrow cells were cultured at a low density (4 × 10
6 cells/a one well of 6-multiwell plate) to generate colonies (colony forming unit-fibroblastic, CFU-F), PTH induced apparent increases in both the total number of CFU-F and the number of ALP-positive CFU-F. The ratio of the latter to the former was significantly higher in the PTH-treated group than in the vehicle-treated group. These findings suggest that the anabolic effect of PTH is, at least in part, due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>7873302</pmid><doi>10.1016/8756-3282(94)90322-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Bone (New York, N.Y.), 1994-11, Vol.15 (6), p.717-723 |
| issn | 8756-3282 1873-2763 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alkaline Phosphatase - metabolism Animals Biological and medical sciences Biomechanical Phenomena Bone Density - drug effects Bone Development - drug effects Bone Marrow - drug effects Bone Marrow Cells Bone marrow stromal cell Cell Count Cell Differentiation - drug effects Cell Division - drug effects Cells, Cultured CFU-F Colony-Forming Units Assay Female Femur - drug effects Fundamental and applied biological sciences. Psychology Hormones. Regulation Humans Injections, Subcutaneous Osteoclasts - drug effects Osteoprogenitor cells Parathyroid hormone Parathyroid Hormone - administration & dosage Parathyroid Hormone - pharmacology Peptide Fragments - administration & dosage Peptide Fragments - pharmacology Rats Rats, Sprague-Dawley Stem Cells - cytology Stem Cells - drug effects Teriparatide Thyroid. Parathyroid. Ultimobranchial body Tibia - drug effects Vertebrates: endocrinology |
| title | Increased bone formation by intermittent parathyroid hormone administration is due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow |
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