A molecular and serologic study of the envelope gene of the British isolate: HIV-1 GB8
The entire envelope gene of a British HIV-1 isolate, GB8, was cloned, sequenced and aligned with those of the reference strains MN, SF2 and IIIB LAI . Three of the viruses (MN, IIIB LAI , GB8) and their recombinant gp120s, were then characterised using an extensive panel of human HIV-1 positive sera...
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| Veröffentlicht in: | Vaccine 1995, Vol.13 (8), p.735-741 |
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| creator | Vella, Cherelyn Smith, Marion H Farrar, Graham H Jones, David H Daniels, Rodney S |
| description | The entire envelope gene of a British HIV-1 isolate, GB8, was cloned, sequenced and aligned with those of the reference strains MN, SF2 and
IIIB
LAI
. Three of the viruses (MN,
IIIB
LAI
, GB8) and their recombinant gp120s, were then characterised using an extensive panel of human HIV-1 positive sera and mapped neutralising monoclonal antibodies (MAbs). Overall, the GB8 env-gene translation product shares 84% homology with those of the reference strains. Across the V3 region homology was greater between GB8 and
SF2
MN
(74.3–88.6%) than
IIIB
LAI
(63.9–66.7%). Accordingly, GB8 was sensitive to V3-specific MAbs which neutralise
MN
SF2
and resistant to those that neutralize
IIIB
LAI
. In the CD4 binding region the central MWQEVGKAMYAPPI was conserved in all viruses but homology in the N-terminus was greater between GB8 and SF2 and
IIIB
LAI
than MN. GB8 and
IIIB
LAI
were sensitive to all MAbs raised against the CD4 binding site whereas MN was resistant to 3 of 4 tested. Human sera obtained from a London-based cohort did not differentiate between GB8 and MN in neutralisation assays, whereas
IIIB
LAI
titres were significantly lower at all stages of disease. These results show that GB8 carries a consensus-like V3 loop and is as representative as MN of HIV-1 viruses circulating in the UK. To our knowledge, GB8 is the only British HIV-1 isolate which has been characterised to date. |
| doi_str_mv | 10.1016/0264-410X(94)00048-R |
| format | Article |
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IIIB
LAI
. Three of the viruses (MN,
IIIB
LAI
, GB8) and their recombinant gp120s, were then characterised using an extensive panel of human HIV-1 positive sera and mapped neutralising monoclonal antibodies (MAbs). Overall, the GB8 env-gene translation product shares 84% homology with those of the reference strains. Across the V3 region homology was greater between GB8 and
SF2
MN
(74.3–88.6%) than
IIIB
LAI
(63.9–66.7%). Accordingly, GB8 was sensitive to V3-specific MAbs which neutralise
MN
SF2
and resistant to those that neutralize
IIIB
LAI
. In the CD4 binding region the central MWQEVGKAMYAPPI was conserved in all viruses but homology in the N-terminus was greater between GB8 and SF2 and
IIIB
LAI
than MN. GB8 and
IIIB
LAI
were sensitive to all MAbs raised against the CD4 binding site whereas MN was resistant to 3 of 4 tested. Human sera obtained from a London-based cohort did not differentiate between GB8 and MN in neutralisation assays, whereas
IIIB
LAI
titres were significantly lower at all stages of disease. These results show that GB8 carries a consensus-like V3 loop and is as representative as MN of HIV-1 viruses circulating in the UK. To our knowledge, GB8 is the only British HIV-1 isolate which has been characterised to date.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/0264-410X(94)00048-R</identifier><identifier>PMID: 7483788</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>AIDS/HIV ; Amino Acid Sequence ; Antibodies, Monoclonal - pharmacology ; Antigen-Antibody Reactions ; Base Sequence ; Biological and medical sciences ; CD4 binding site ; Cells, Cultured ; Fundamental and applied biological sciences. Psychology ; GB8 rgp120 ; Genes, env ; HIV Envelope Protein gp120 - genetics ; HIV Envelope Protein gp120 - immunology ; HIV Seropositivity - immunology ; HIV-1 - genetics ; HIV-1 - immunology ; HIV-1 - isolation & purification ; HIV-1GB8 British isolate sequence ; human immunodeficiency virus 1 ; Humans ; Immune Sera - pharmacology ; Microbiology ; Molecular Sequence Data ; Morphology, structure, chemical composition, physicochemical properties ; neutralisation V3 ; Neutralization Tests ; Peptides - immunology ; Recombinant Proteins - immunology ; serology ; United Kingdom ; Virology</subject><ispartof>Vaccine, 1995, Vol.13 (8), p.735-741</ispartof><rights>1995</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-914ee08275071369afbff27fdfc6c8e05d355769a1d10f1a19ee3c07bf2dbb1c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0264-410X(94)00048-R$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3017773$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7483788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vella, Cherelyn</creatorcontrib><creatorcontrib>Smith, Marion H</creatorcontrib><creatorcontrib>Farrar, Graham H</creatorcontrib><creatorcontrib>Jones, David H</creatorcontrib><creatorcontrib>Daniels, Rodney S</creatorcontrib><title>A molecular and serologic study of the envelope gene of the British isolate: HIV-1 GB8</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>The entire envelope gene of a British HIV-1 isolate, GB8, was cloned, sequenced and aligned with those of the reference strains MN, SF2 and
IIIB
LAI
. Three of the viruses (MN,
IIIB
LAI
, GB8) and their recombinant gp120s, were then characterised using an extensive panel of human HIV-1 positive sera and mapped neutralising monoclonal antibodies (MAbs). Overall, the GB8 env-gene translation product shares 84% homology with those of the reference strains. Across the V3 region homology was greater between GB8 and
SF2
MN
(74.3–88.6%) than
IIIB
LAI
(63.9–66.7%). Accordingly, GB8 was sensitive to V3-specific MAbs which neutralise
MN
SF2
and resistant to those that neutralize
IIIB
LAI
. In the CD4 binding region the central MWQEVGKAMYAPPI was conserved in all viruses but homology in the N-terminus was greater between GB8 and SF2 and
IIIB
LAI
than MN. GB8 and
IIIB
LAI
were sensitive to all MAbs raised against the CD4 binding site whereas MN was resistant to 3 of 4 tested. Human sera obtained from a London-based cohort did not differentiate between GB8 and MN in neutralisation assays, whereas
IIIB
LAI
titres were significantly lower at all stages of disease. These results show that GB8 carries a consensus-like V3 loop and is as representative as MN of HIV-1 viruses circulating in the UK. To our knowledge, GB8 is the only British HIV-1 isolate which has been characterised to date.</description><subject>AIDS/HIV</subject><subject>Amino Acid Sequence</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Antigen-Antibody Reactions</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CD4 binding site</subject><subject>Cells, Cultured</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GB8 rgp120</subject><subject>Genes, env</subject><subject>HIV Envelope Protein gp120 - genetics</subject><subject>HIV Envelope Protein gp120 - immunology</subject><subject>HIV Seropositivity - immunology</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - immunology</subject><subject>HIV-1 - isolation & purification</subject><subject>HIV-1GB8 British isolate sequence</subject><subject>human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Immune Sera - pharmacology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>neutralisation V3</subject><subject>Neutralization Tests</subject><subject>Peptides - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>serology</subject><subject>United Kingdom</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkNFKHDEUhkOp2NX2DVrIRSn2YmrOJDPJ9KKg0qogCNJK70ImOdFIdrJNZgTfvrPddS_t1YFzvvPz8xHyHtgXYNAes7oVlQD2-6gTnxljQlU3r8gClORV3YB6TRY75A05KOVhhhoO3T7Zl0JxqdSC3J7QZYpop2gyNYOjBXOK6S5YWsbJPdHk6XiPFIdHjGmF9A4HfF6e5jCGck9DSdGM-JVeXN5WQM9P1Vuy500s-G47D8mvH99_nl1UV9fnl2cnV5UVIMeqA4HIVC0bJoG3nfG997X0ztvWKmSN400j5z04YB4MdIjcMtn72vU9WH5IPm1yVzn9mbCMehmKxRjNgGkqWkophGra_4LQqpaLhs2g2IA2p1Iyer3KYWnykwam1971WqpeS9Wd0P-865v57cM2f-qX6HZPW9Hz_eP2boo10Wcz2FB2GGcwV-Uz9m2D4SztMWDWxQYcLLqQ0Y7apfByj79oqJ2B</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Vella, Cherelyn</creator><creator>Smith, Marion H</creator><creator>Farrar, Graham H</creator><creator>Jones, David H</creator><creator>Daniels, Rodney S</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>A molecular and serologic study of the envelope gene of the British isolate: HIV-1 GB8</title><author>Vella, Cherelyn ; Smith, Marion H ; Farrar, Graham H ; Jones, David H ; Daniels, Rodney S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-914ee08275071369afbff27fdfc6c8e05d355769a1d10f1a19ee3c07bf2dbb1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AIDS/HIV</topic><topic>Amino Acid Sequence</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>Antigen-Antibody Reactions</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CD4 binding site</topic><topic>Cells, Cultured</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GB8 rgp120</topic><topic>Genes, env</topic><topic>HIV Envelope Protein gp120 - genetics</topic><topic>HIV Envelope Protein gp120 - immunology</topic><topic>HIV Seropositivity - immunology</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - immunology</topic><topic>HIV-1 - isolation & purification</topic><topic>HIV-1GB8 British isolate sequence</topic><topic>human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Immune Sera - pharmacology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>neutralisation V3</topic><topic>Neutralization Tests</topic><topic>Peptides - immunology</topic><topic>Recombinant Proteins - immunology</topic><topic>serology</topic><topic>United Kingdom</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vella, Cherelyn</creatorcontrib><creatorcontrib>Smith, Marion H</creatorcontrib><creatorcontrib>Farrar, Graham H</creatorcontrib><creatorcontrib>Jones, David H</creatorcontrib><creatorcontrib>Daniels, Rodney S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vella, Cherelyn</au><au>Smith, Marion H</au><au>Farrar, Graham H</au><au>Jones, David H</au><au>Daniels, Rodney S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A molecular and serologic study of the envelope gene of the British isolate: HIV-1 GB8</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>1995</date><risdate>1995</risdate><volume>13</volume><issue>8</issue><spage>735</spage><epage>741</epage><pages>735-741</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>The entire envelope gene of a British HIV-1 isolate, GB8, was cloned, sequenced and aligned with those of the reference strains MN, SF2 and
IIIB
LAI
. Three of the viruses (MN,
IIIB
LAI
, GB8) and their recombinant gp120s, were then characterised using an extensive panel of human HIV-1 positive sera and mapped neutralising monoclonal antibodies (MAbs). Overall, the GB8 env-gene translation product shares 84% homology with those of the reference strains. Across the V3 region homology was greater between GB8 and
SF2
MN
(74.3–88.6%) than
IIIB
LAI
(63.9–66.7%). Accordingly, GB8 was sensitive to V3-specific MAbs which neutralise
MN
SF2
and resistant to those that neutralize
IIIB
LAI
. In the CD4 binding region the central MWQEVGKAMYAPPI was conserved in all viruses but homology in the N-terminus was greater between GB8 and SF2 and
IIIB
LAI
than MN. GB8 and
IIIB
LAI
were sensitive to all MAbs raised against the CD4 binding site whereas MN was resistant to 3 of 4 tested. Human sera obtained from a London-based cohort did not differentiate between GB8 and MN in neutralisation assays, whereas
IIIB
LAI
titres were significantly lower at all stages of disease. These results show that GB8 carries a consensus-like V3 loop and is as representative as MN of HIV-1 viruses circulating in the UK. To our knowledge, GB8 is the only British HIV-1 isolate which has been characterised to date.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>7483788</pmid><doi>10.1016/0264-410X(94)00048-R</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 1995, Vol.13 (8), p.735-741 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77744856 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | AIDS/HIV Amino Acid Sequence Antibodies, Monoclonal - pharmacology Antigen-Antibody Reactions Base Sequence Biological and medical sciences CD4 binding site Cells, Cultured Fundamental and applied biological sciences. Psychology GB8 rgp120 Genes, env HIV Envelope Protein gp120 - genetics HIV Envelope Protein gp120 - immunology HIV Seropositivity - immunology HIV-1 - genetics HIV-1 - immunology HIV-1 - isolation & purification HIV-1GB8 British isolate sequence human immunodeficiency virus 1 Humans Immune Sera - pharmacology Microbiology Molecular Sequence Data Morphology, structure, chemical composition, physicochemical properties neutralisation V3 Neutralization Tests Peptides - immunology Recombinant Proteins - immunology serology United Kingdom Virology |
| title | A molecular and serologic study of the envelope gene of the British isolate: HIV-1 GB8 |
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