Expression of a Recombinant Human Glycosyltransferase from a Synthetic Gene and its Utilization for Synthesis of the Human Blood Group B Trisaccharide

A 1034‐bp synthetic gene encoding the human blood group B glycosyltransferase, which catalyzes the transfer of galactose from UDP‐Gal to Fucα(l‐2)Galβ‐OR to give the blood group B determinant Galα(l‐3)[Fucα(1‐2)]Gal β‐OR (where R is a glycoprotein or glycolipid), has been expressed in Escherichia co...

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Veröffentlicht in:	European journal of biochemistry 1995-11, Vol.234 (1), p.323-328
Hauptverfasser:	Seto, Nina O. L., Palcic, Monica M., Hindsgaul, Ole, Bundle, David R., Narang, Saran A.
Format:	Artikel
Sprache:	eng
Schlagworte:	ABO Blood-Group System Amino Acid Sequence Base Sequence blood group antigen Carbohydrate Sequence Cloning, Molecular DNA, Recombinant enzymic synthesis Escherichia coli - genetics Galactosyltransferases - genetics Genes, Synthetic glycosyltransferases Humans Kinetics Molecular Sequence Data recombinant Recombinant Proteins - genetics Recombinant Proteins - metabolism synthetic genes Trisaccharides - biosynthesis
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