Prevalence of Colonization Factor Antigens (CFAs) and Adherence to HeLa Cells in Enterotoxigenic Escherichia coli Isolated from Feces of Children in São Paulo
Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in São Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS...
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| Veröffentlicht in: | MICROBIOLOGY and IMMUNOLOGY 1994, Vol.38(9), pp.695-701 |
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| creator | Guth, Beatriz Ernestina Cabilio Aguiar, Eliana Gonçalves Griffin, Patricia Marie da Silva Ramos, Sonia Regina Testa Gomes, Tânia Aparecida Tardelli |
| description | Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in São Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/II, CFA/III, and CS5CS6 and CS6 of CFA/IV were used. CFAs were identified in 43% of the ETEC strains: 40% of the strains with CFAs harbored CFA/I, 24% carried CFA/II (CS1CS3), 24% carried CFA/IV (CS6), and 12% carried CFA/IV (CS5CS6). CFAs occurred mainly among ETEC strains producing only heat-stable (ST-I) enterotoxin and in strains also producing heat-labile toxin (LT-I). No ETEC strains tested expressed CFA/III. A marked change in serotypes of ST-I-producing strains was found in São Paulo between 1979 and 1990. Adherence to HeLa cells was detected in 14% of the ETEC strains. All of them had a diffuse adherence pattern and produced only ST-I, and 88% carried CS6 antigen. |
| doi_str_mv | 10.1111/j.1348-0421.1994.tb01844.x |
| format | Article |
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Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/II, CFA/III, and CS5CS6 and CS6 of CFA/IV were used. CFAs were identified in 43% of the ETEC strains: 40% of the strains with CFAs harbored CFA/I, 24% carried CFA/II (CS1CS3), 24% carried CFA/IV (CS6), and 12% carried CFA/IV (CS5CS6). CFAs occurred mainly among ETEC strains producing only heat-stable (ST-I) enterotoxin and in strains also producing heat-labile toxin (LT-I). No ETEC strains tested expressed CFA/III. A marked change in serotypes of ST-I-producing strains was found in São Paulo between 1979 and 1990. Adherence to HeLa cells was detected in 14% of the ETEC strains. All of them had a diffuse adherence pattern and produced only ST-I, and 88% carried CS6 antigen.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1994.tb01844.x</identifier><identifier>PMID: 7854210</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Adherence ; Animals ; Antigens, Bacterial - analysis ; Antigens, Bacterial - immunology ; Bacterial Adhesion ; Bacterial Proteins - analysis ; Bacterial Proteins - immunology ; Bacteriology ; Biological and medical sciences ; Brazil - epidemiology ; Child, Preschool ; Colonization factor antigens ; Diarrhea ; Diarrhea - epidemiology ; Diarrhea - microbiology ; Enterotoxins ; Escherichia coli ; Escherichia coli - immunology ; Escherichia coli - isolation & purification ; Escherichia coli - physiology ; Feces - microbiology ; Fimbriae Proteins ; Fundamental and applied biological sciences. Psychology ; HeLa Cells - metabolism ; Hemagglutination Tests ; Humans ; Immunodiffusion ; Infant ; Microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Phenotype ; Prevalence ; Rabbits</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 1994, Vol.38(9), pp.695-701</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6374-11894cf5e89542ec22eaf8719a558077065c1660191240b8caadfbef9854f5263</citedby><cites>FETCH-LOGICAL-c6374-11894cf5e89542ec22eaf8719a558077065c1660191240b8caadfbef9854f5263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3323307$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7854210$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guth, Beatriz Ernestina Cabilio</creatorcontrib><creatorcontrib>Aguiar, Eliana Gonçalves</creatorcontrib><creatorcontrib>Griffin, Patricia Marie</creatorcontrib><creatorcontrib>da Silva Ramos, Sonia Regina Testa</creatorcontrib><creatorcontrib>Gomes, Tânia Aparecida Tardelli</creatorcontrib><title>Prevalence of Colonization Factor Antigens (CFAs) and Adherence to HeLa Cells in Enterotoxigenic Escherichia coli Isolated from Feces of Children in São Paulo</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>Microbiology and Immunology</addtitle><description>Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in São Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/II, CFA/III, and CS5CS6 and CS6 of CFA/IV were used. CFAs were identified in 43% of the ETEC strains: 40% of the strains with CFAs harbored CFA/I, 24% carried CFA/II (CS1CS3), 24% carried CFA/IV (CS6), and 12% carried CFA/IV (CS5CS6). CFAs occurred mainly among ETEC strains producing only heat-stable (ST-I) enterotoxin and in strains also producing heat-labile toxin (LT-I). No ETEC strains tested expressed CFA/III. A marked change in serotypes of ST-I-producing strains was found in São Paulo between 1979 and 1990. Adherence to HeLa cells was detected in 14% of the ETEC strains. All of them had a diffuse adherence pattern and produced only ST-I, and 88% carried CS6 antigen.</description><subject>Adherence</subject><subject>Animals</subject><subject>Antigens, Bacterial - analysis</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial Adhesion</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Brazil - epidemiology</subject><subject>Child, Preschool</subject><subject>Colonization factor antigens</subject><subject>Diarrhea</subject><subject>Diarrhea - epidemiology</subject><subject>Diarrhea - microbiology</subject><subject>Enterotoxins</subject><subject>Escherichia coli</subject><subject>Escherichia coli - immunology</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli - physiology</subject><subject>Feces - microbiology</subject><subject>Fimbriae Proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells - metabolism</subject><subject>Hemagglutination Tests</subject><subject>Humans</subject><subject>Immunodiffusion</subject><subject>Infant</subject><subject>Microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Phenotype</subject><subject>Prevalence</subject><subject>Rabbits</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVktFu0zAUhiMEGmXwCEgWQmhcpNixE9tcUVXt1qmDSgPBneU6J6tLGg87hY6X4YJH4cVwmqriCkQukkjnO599_DtJnhE8JPF5tR4SykSKWUaGREo2bJeYCMaGu3vJ4Fi6nwwwFXmaFxg_TB6FsMY445lgJ8kJF3kk8CD5sfDwVdfQGECuQmNXu8Z-1611DZpq0zqPRk1rb6AJ6Gw8HYWXSDclGpUr8Pum1qELmGs0hroOyDZo0rTgXet2XZM1aBJMZK1ZWY2Mqy2aBVfrFkpUebdBUzAQ9kuvbF1GZ-e4_vXToYXe1u5x8qDSdYAnh-9p8mE6eT--SOfvzmfj0Tw1BeUsJURIZqochIyDgcky0JXgROo8F5hzXOSGFAUmkmQML4XRuqyWUMl4DlWeFfQ0edF7b737soXQqo0NJs6kG3DboDjnDEss_gmSQlDJaWc8-zvIZFbE3WQsoq971HgXgodK3Xq70f5OEay6xNVadbGqLlbVJa4OiatdbH56WGe73EB5bD1EHOvPD3UdjK4rrxtjwxGjNKMU84i96bFvtoa7_9iAuppd7X-jYtIr1qHVN3B0aN9aU4PaxJtjieRcUaFk_ypkfqyblfYKmuhJe48NLez-0HxWBac8Vx_fnqvLy0X-qSiu4wC_AZBE7_0</recordid><startdate>19940101</startdate><enddate>19940101</enddate><creator>Guth, Beatriz Ernestina Cabilio</creator><creator>Aguiar, Eliana Gonçalves</creator><creator>Griffin, Patricia Marie</creator><creator>da Silva Ramos, Sonia Regina Testa</creator><creator>Gomes, Tânia Aparecida Tardelli</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940101</creationdate><title>Prevalence of Colonization Factor Antigens (CFAs) and Adherence to HeLa Cells in Enterotoxigenic Escherichia coli Isolated from Feces of Children in São Paulo</title><author>Guth, Beatriz Ernestina Cabilio ; Aguiar, Eliana Gonçalves ; Griffin, Patricia Marie ; da Silva Ramos, Sonia Regina Testa ; Gomes, Tânia Aparecida Tardelli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6374-11894cf5e89542ec22eaf8719a558077065c1660191240b8caadfbef9854f5263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adherence</topic><topic>Animals</topic><topic>Antigens, Bacterial - analysis</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial Adhesion</topic><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Brazil - epidemiology</topic><topic>Child, Preschool</topic><topic>Colonization factor antigens</topic><topic>Diarrhea</topic><topic>Diarrhea - epidemiology</topic><topic>Diarrhea - microbiology</topic><topic>Enterotoxins</topic><topic>Escherichia coli</topic><topic>Escherichia coli - immunology</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli - physiology</topic><topic>Feces - microbiology</topic><topic>Fimbriae Proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa Cells - metabolism</topic><topic>Hemagglutination Tests</topic><topic>Humans</topic><topic>Immunodiffusion</topic><topic>Infant</topic><topic>Microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Phenotype</topic><topic>Prevalence</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guth, Beatriz Ernestina Cabilio</creatorcontrib><creatorcontrib>Aguiar, Eliana Gonçalves</creatorcontrib><creatorcontrib>Griffin, Patricia Marie</creatorcontrib><creatorcontrib>da Silva Ramos, Sonia Regina Testa</creatorcontrib><creatorcontrib>Gomes, Tânia Aparecida Tardelli</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guth, Beatriz Ernestina Cabilio</au><au>Aguiar, Eliana Gonçalves</au><au>Griffin, Patricia Marie</au><au>da Silva Ramos, Sonia Regina Testa</au><au>Gomes, Tânia Aparecida Tardelli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of Colonization Factor Antigens (CFAs) and Adherence to HeLa Cells in Enterotoxigenic Escherichia coli Isolated from Feces of Children in São Paulo</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1994-01-01</date><risdate>1994</risdate><volume>38</volume><issue>9</issue><spage>695</spage><epage>701</epage><pages>695-701</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in São Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/II, CFA/III, and CS5CS6 and CS6 of CFA/IV were used. CFAs were identified in 43% of the ETEC strains: 40% of the strains with CFAs harbored CFA/I, 24% carried CFA/II (CS1CS3), 24% carried CFA/IV (CS6), and 12% carried CFA/IV (CS5CS6). CFAs occurred mainly among ETEC strains producing only heat-stable (ST-I) enterotoxin and in strains also producing heat-labile toxin (LT-I). No ETEC strains tested expressed CFA/III. A marked change in serotypes of ST-I-producing strains was found in São Paulo between 1979 and 1990. Adherence to HeLa cells was detected in 14% of the ETEC strains. All of them had a diffuse adherence pattern and produced only ST-I, and 88% carried CS6 antigen.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>7854210</pmid><doi>10.1111/j.1348-0421.1994.tb01844.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | MICROBIOLOGY and IMMUNOLOGY, 1994, Vol.38(9), pp.695-701 |
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| language | eng |
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| source | Freely Accessible Japanese Titles - check A-Z of ejournals; J-STAGE Free; MEDLINE; Alma/SFX Local Collection |
| subjects | Adherence Animals Antigens, Bacterial - analysis Antigens, Bacterial - immunology Bacterial Adhesion Bacterial Proteins - analysis Bacterial Proteins - immunology Bacteriology Biological and medical sciences Brazil - epidemiology Child, Preschool Colonization factor antigens Diarrhea Diarrhea - epidemiology Diarrhea - microbiology Enterotoxins Escherichia coli Escherichia coli - immunology Escherichia coli - isolation & purification Escherichia coli - physiology Feces - microbiology Fimbriae Proteins Fundamental and applied biological sciences. Psychology HeLa Cells - metabolism Hemagglutination Tests Humans Immunodiffusion Infant Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Phenotype Prevalence Rabbits |
| title | Prevalence of Colonization Factor Antigens (CFAs) and Adherence to HeLa Cells in Enterotoxigenic Escherichia coli Isolated from Feces of Children in São Paulo |
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