Energy economy of human uterine muscle strips under different in vitro conditions and its dependence on tissue redox potential
Objective: To study the energy economy of human myometrium in vitro. Study design: Myometric biopsies were kept for 10 min or 4 h in a buffer solution at +4°C before preparation into ‘early’ and ‘late’ strips, respectively. The strips were perifused at +37°C for different periods of time. Adenylates...
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| Veröffentlicht in: | European journal of obstetrics & gynecology and reproductive biology 1995-09, Vol.62 (1), p.115-119 |
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| container_title | European journal of obstetrics & gynecology and reproductive biology |
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| creator | Wedenberg, Kaj Ronquist, Gunnar Ulmsten, Ulf Waldenström, Anders |
| description | Objective: To study the energy economy of human myometrium in vitro.
Study design: Myometric biopsies were kept for 10 min or 4 h in a buffer solution at +4°C before preparation into ‘early’ and ‘late’ strips, respectively. The strips were perifused at +37°C for different periods of time. Adenylates, energy charge (EC), adenosine and NAD
+ were determined in individual strips.
Results: Mean EC and ATP values were low, the ‘late’ strips displaying 5% and 20% lower values than the ‘early’ strips, respectively. During perifusion the EC increased slightly, ADP decreased significantly and the strips began to contract. A correlation fitting a multiplicative function was evident between EC and NAD
+ (
r = 0.89,
P < 0.001).
Conclusion: Human myometrium, being operative at an unusually low EC level, maintained its energy economizing and contractile functions in vitro for 4 h, displaying a tight relationship between EC and NAD
+, suggestive of a compromised energy balance. |
| doi_str_mv | 10.1016/0301-2115(95)02171-3 |
| format | Article |
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Study design: Myometric biopsies were kept for 10 min or 4 h in a buffer solution at +4°C before preparation into ‘early’ and ‘late’ strips, respectively. The strips were perifused at +37°C for different periods of time. Adenylates, energy charge (EC), adenosine and NAD
+ were determined in individual strips.
Results: Mean EC and ATP values were low, the ‘late’ strips displaying 5% and 20% lower values than the ‘early’ strips, respectively. During perifusion the EC increased slightly, ADP decreased significantly and the strips began to contract. A correlation fitting a multiplicative function was evident between EC and NAD
+ (
r = 0.89,
P < 0.001).
Conclusion: Human myometrium, being operative at an unusually low EC level, maintained its energy economizing and contractile functions in vitro for 4 h, displaying a tight relationship between EC and NAD
+, suggestive of a compromised energy balance.</description><identifier>ISSN: 0301-2115</identifier><identifier>EISSN: 1872-7654</identifier><identifier>DOI: 10.1016/0301-2115(95)02171-3</identifier><identifier>PMID: 7493691</identifier><identifier>CODEN: EOGRAL</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Adenosine Diphosphate - metabolism ; Adenosine Triphosphate - metabolism ; Adult ; ATP ; Biological and medical sciences ; Electrodiagnosis. Electric activity recording ; Energy charge ; Energy Metabolism ; Female ; Human ; Humans ; In Vitro Techniques ; Investigative techniques, diagnostic techniques (general aspects) ; Kinetics ; Medical sciences ; Miscellaneous. Technology ; Myometrium ; Myometrium - metabolism ; NAD ; NAD - metabolism ; Oxidation-Reduction ; Pregnancy ; Uterine Contraction - physiology ; Uterus</subject><ispartof>European journal of obstetrics & gynecology and reproductive biology, 1995-09, Vol.62 (1), p.115-119</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-697818c38a221ebca46e506ebb22f72a89af85aa20619eff20c4b17b1ab8ad5e3</citedby><cites>FETCH-LOGICAL-c386t-697818c38a221ebca46e506ebb22f72a89af85aa20619eff20c4b17b1ab8ad5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0301-2115(95)02171-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3656062$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7493691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wedenberg, Kaj</creatorcontrib><creatorcontrib>Ronquist, Gunnar</creatorcontrib><creatorcontrib>Ulmsten, Ulf</creatorcontrib><creatorcontrib>Waldenström, Anders</creatorcontrib><title>Energy economy of human uterine muscle strips under different in vitro conditions and its dependence on tissue redox potential</title><title>European journal of obstetrics & gynecology and reproductive biology</title><addtitle>Eur J Obstet Gynecol Reprod Biol</addtitle><description>Objective: To study the energy economy of human myometrium in vitro.
Study design: Myometric biopsies were kept for 10 min or 4 h in a buffer solution at +4°C before preparation into ‘early’ and ‘late’ strips, respectively. The strips were perifused at +37°C for different periods of time. Adenylates, energy charge (EC), adenosine and NAD
+ were determined in individual strips.
Results: Mean EC and ATP values were low, the ‘late’ strips displaying 5% and 20% lower values than the ‘early’ strips, respectively. During perifusion the EC increased slightly, ADP decreased significantly and the strips began to contract. A correlation fitting a multiplicative function was evident between EC and NAD
+ (
r = 0.89,
P < 0.001).
Conclusion: Human myometrium, being operative at an unusually low EC level, maintained its energy economizing and contractile functions in vitro for 4 h, displaying a tight relationship between EC and NAD
+, suggestive of a compromised energy balance.</description><subject>Adenosine Diphosphate - metabolism</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Adult</subject><subject>ATP</subject><subject>Biological and medical sciences</subject><subject>Electrodiagnosis. Electric activity recording</subject><subject>Energy charge</subject><subject>Energy Metabolism</subject><subject>Female</subject><subject>Human</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>Miscellaneous. Technology</subject><subject>Myometrium</subject><subject>Myometrium - metabolism</subject><subject>NAD</subject><subject>NAD - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Pregnancy</subject><subject>Uterine Contraction - physiology</subject><subject>Uterus</subject><issn>0301-2115</issn><issn>1872-7654</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo6-zqP1DIQUQPrfnoJN2XBVl2VVjwoueQTioa6U7aJL04F3-7GWeYo3WpgnreongQekHJO0qofE84oR2jVLwZxVvCqKIdf4R2dFCsU1L0j9HujDxFl6X8JK04Hy_QhepHLke6Q39uI-Tveww2xbTscfL4x7aYiLcKOUTAy1bsDLjUHNaCt-ggYxe8hwyx4hDxQ6g54RZ3oYYUCzbR4VALdrBCw6MFnCKuoZQNcAaXfuM11ZYOZn6GnngzF3h-6lfo293t15tP3f2Xj59vPtx3lg-ydnJUAx3abBijMFnTSxBEwjQx5hUzw2j8IIxhRNIRvGfE9hNVEzXTYJwAfoVeH--uOf3aoFS9hGJhnk2EtBWtlOIj5aKB_RG0OZWSwes1h8XkvaZEH7Trg1N9cKpHof9p17zFXp7ub9MC7hw6eW77V6e9KdbMPptoQzljXApJJGvY9RGD5uIhQNbFhoNBFzLYql0K___jL536oN8</recordid><startdate>19950901</startdate><enddate>19950901</enddate><creator>Wedenberg, Kaj</creator><creator>Ronquist, Gunnar</creator><creator>Ulmsten, Ulf</creator><creator>Waldenström, Anders</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950901</creationdate><title>Energy economy of human uterine muscle strips under different in vitro conditions and its dependence on tissue redox potential</title><author>Wedenberg, Kaj ; Ronquist, Gunnar ; Ulmsten, Ulf ; Waldenström, Anders</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-697818c38a221ebca46e506ebb22f72a89af85aa20619eff20c4b17b1ab8ad5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adenosine Diphosphate - metabolism</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Adult</topic><topic>ATP</topic><topic>Biological and medical sciences</topic><topic>Electrodiagnosis. Electric activity recording</topic><topic>Energy charge</topic><topic>Energy Metabolism</topic><topic>Female</topic><topic>Human</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>Miscellaneous. Technology</topic><topic>Myometrium</topic><topic>Myometrium - metabolism</topic><topic>NAD</topic><topic>NAD - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Pregnancy</topic><topic>Uterine Contraction - physiology</topic><topic>Uterus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wedenberg, Kaj</creatorcontrib><creatorcontrib>Ronquist, Gunnar</creatorcontrib><creatorcontrib>Ulmsten, Ulf</creatorcontrib><creatorcontrib>Waldenström, Anders</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of obstetrics & gynecology and reproductive biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wedenberg, Kaj</au><au>Ronquist, Gunnar</au><au>Ulmsten, Ulf</au><au>Waldenström, Anders</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Energy economy of human uterine muscle strips under different in vitro conditions and its dependence on tissue redox potential</atitle><jtitle>European journal of obstetrics & gynecology and reproductive biology</jtitle><addtitle>Eur J Obstet Gynecol Reprod Biol</addtitle><date>1995-09-01</date><risdate>1995</risdate><volume>62</volume><issue>1</issue><spage>115</spage><epage>119</epage><pages>115-119</pages><issn>0301-2115</issn><eissn>1872-7654</eissn><coden>EOGRAL</coden><abstract>Objective: To study the energy economy of human myometrium in vitro.
Study design: Myometric biopsies were kept for 10 min or 4 h in a buffer solution at +4°C before preparation into ‘early’ and ‘late’ strips, respectively. The strips were perifused at +37°C for different periods of time. Adenylates, energy charge (EC), adenosine and NAD
+ were determined in individual strips.
Results: Mean EC and ATP values were low, the ‘late’ strips displaying 5% and 20% lower values than the ‘early’ strips, respectively. During perifusion the EC increased slightly, ADP decreased significantly and the strips began to contract. A correlation fitting a multiplicative function was evident between EC and NAD
+ (
r = 0.89,
P < 0.001).
Conclusion: Human myometrium, being operative at an unusually low EC level, maintained its energy economizing and contractile functions in vitro for 4 h, displaying a tight relationship between EC and NAD
+, suggestive of a compromised energy balance.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>7493691</pmid><doi>10.1016/0301-2115(95)02171-3</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0301-2115 |
| ispartof | European journal of obstetrics & gynecology and reproductive biology, 1995-09, Vol.62 (1), p.115-119 |
| issn | 0301-2115 1872-7654 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adenosine Diphosphate - metabolism Adenosine Triphosphate - metabolism Adult ATP Biological and medical sciences Electrodiagnosis. Electric activity recording Energy charge Energy Metabolism Female Human Humans In Vitro Techniques Investigative techniques, diagnostic techniques (general aspects) Kinetics Medical sciences Miscellaneous. Technology Myometrium Myometrium - metabolism NAD NAD - metabolism Oxidation-Reduction Pregnancy Uterine Contraction - physiology Uterus |
| title | Energy economy of human uterine muscle strips under different in vitro conditions and its dependence on tissue redox potential |
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