The Expression of Chloramphenicol Acetyltransferase in Mosquitoes and Mosquito Cells Using a Packaged Sindbis Replicon System

Sindbis (SIN) replicon virus was used to express chloramphenicol acetyltransferase (CAT) in Aedes albopictus (C6/36) cells and Aedes triseriatus mosquitoes. RNA transcribed in vitro from a SIN replicon plasmid (pSINrep5/CAT) and from SIN virus helper constructs (pDH-EB or pDH(26S)5′SIN) was coelectr...

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Veröffentlicht in:	Experimental parasitology 1995-11, Vol.81 (3), p.394-403
Hauptverfasser:	Kamrud, K.I., Powers, A.M., Higgs, S., Olson, K.E., Blair, C.D., Carlson, J.O., Beaty, B.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	ACILTRANSFERASA ACYLTRANSFERASE ACYLTRANSFERASES Aedes Aedes - enzymology Aedes - genetics AEDES ALBOPICTUS AEDES TRISERIATUS ALPHAVIRUS Animals BIOASSAYS BODY PARTS CELLS CELLULE CELULAS Chloramphenicol O-Acetyltransferase - biosynthesis Chloramphenicol O-Acetyltransferase - genetics Culicidae Diptera DOSAGE BIOLOGIQUE ENSAYO BIOLOGICO EXPERIMENTACION IN VITRO EXPERIMENTATION IN VITRO EXPRESION GENICA EXPRESSION DES GENES Female Fluorescent Antibody Technique, Indirect Freshwater GENE EXPRESSION Gene Expression Regulation, Enzymologic IN VITRO EXPERIMENTATION Insect Vectors - enzymology Insect Vectors - genetics ORGANS PARTES DEL CUERPO PARTIE DU CORPS Replicon RNA, Viral - genetics SINDBIS VIRUS Sindbis Virus - genetics
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