Synthesis and Intracellular Transport of Aminoglycerophospholipids in Permeabilized Cells of the Yeast, Saccharomyces cerevisiae(∗)
The sequence of biosynthetic steps from phosphatidylserine to phosphatidylethanolamine (via decarboxylation) and then phosphatidylcholine (via methylation) is linked to the intracellular transport of these aminoglycerophospholipids. Using a [3H]serine precursor and permeabilized yeast cells, it is p...
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| Veröffentlicht in: | The Journal of biological chemistry 1995-12, Vol.270 (50), p.29836-29842 |
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| creator | Achleitner, Georg Zweytick, Dagmar Trotter, Pamela J. Voelker, Dennis R. Daum, Günther |
| description | The sequence of biosynthetic steps from phosphatidylserine to phosphatidylethanolamine (via decarboxylation) and then phosphatidylcholine (via methylation) is linked to the intracellular transport of these aminoglycerophospholipids. Using a [3H]serine precursor and permeabilized yeast cells, it is possible to follow the synthesis of each of the aminoglycerophospholipids and examine the requirements for their interorganelle transport. This experimental approach reveals that in permeabilized cells newly synthesized phosphatidylserine is readily translocated to the locus of phosphatidylserine decarboxylase 1 in the mitochondria but not to the locus of phosphatidylserine decarboxylase 2 in the Golgi and vacuoles. Phosphatidylserine transport to the mitochondria is ATP independent and exhibits no requirements for cytosolic factors. The phosphatidylethanolamine formed in the mitochondria is exported to the locus of the methyltransferases (principally the endoplasmic reticulum) and converted to phosphatidylcholine. The export of phosphatidylethanolamine requires ATP but not any other cytosolic factors and is not obligately coupled to methyltransferase activity. The above described lipid transport reactions also occur in permeabilized cells that have been disrupted by homogenization, indicating that the processes are extremely efficient and may be dependent upon stable structural elements between organelles. |
| doi_str_mv | 10.1074/jbc.270.50.29836 |
| format | Article |
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Using a [3H]serine precursor and permeabilized yeast cells, it is possible to follow the synthesis of each of the aminoglycerophospholipids and examine the requirements for their interorganelle transport. This experimental approach reveals that in permeabilized cells newly synthesized phosphatidylserine is readily translocated to the locus of phosphatidylserine decarboxylase 1 in the mitochondria but not to the locus of phosphatidylserine decarboxylase 2 in the Golgi and vacuoles. Phosphatidylserine transport to the mitochondria is ATP independent and exhibits no requirements for cytosolic factors. The phosphatidylethanolamine formed in the mitochondria is exported to the locus of the methyltransferases (principally the endoplasmic reticulum) and converted to phosphatidylcholine. The export of phosphatidylethanolamine requires ATP but not any other cytosolic factors and is not obligately coupled to methyltransferase activity. The above described lipid transport reactions also occur in permeabilized cells that have been disrupted by homogenization, indicating that the processes are extremely efficient and may be dependent upon stable structural elements between organelles.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.270.50.29836</identifier><identifier>PMID: 8530379</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; BIOCHIMIE ; Biological Transport ; BIOQUIMICA ; CEFALINAS ; Cell Fractionation ; Cell Membrane Permeability ; CEPHALINE ; Cytosol - metabolism ; Kinetics ; LECITHINE ; LECITINAS ; LIASAS ; LIPOGENESE ; LIPOGENESIS ; LYASE ; Methyltransferases - metabolism ; Microsomes - metabolism ; Mitochondria - metabolism ; MITOCHONDRIE ; MITOCONDRIA ; ORGANITE CELLULAIRE ; ORGANULOS CITOPLASMICOS ; Phosphatidylcholines - biosynthesis ; Phosphatidylcholines - isolation & purification ; Phosphatidylcholines - metabolism ; Phosphatidylethanolamines - biosynthesis ; Phosphatidylethanolamines - isolation & purification ; Phosphatidylethanolamines - metabolism ; Phosphatidylserines - biosynthesis ; Phosphatidylserines - isolation & purification ; Phosphatidylserines - metabolism ; Radioisotope Dilution Technique ; RETICULO ENDOPLASMATICO ; RETICULUM ENDOPLASMIQUE ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - metabolism ; Serine - metabolism ; Spheroplasts - metabolism ; TRANSFERASAS ; TRANSFERASE ; Tritium</subject><ispartof>The Journal of biological chemistry, 1995-12, Vol.270 (50), p.29836-29842</ispartof><rights>1995 © 1995 ASBMB. 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Using a [3H]serine precursor and permeabilized yeast cells, it is possible to follow the synthesis of each of the aminoglycerophospholipids and examine the requirements for their interorganelle transport. This experimental approach reveals that in permeabilized cells newly synthesized phosphatidylserine is readily translocated to the locus of phosphatidylserine decarboxylase 1 in the mitochondria but not to the locus of phosphatidylserine decarboxylase 2 in the Golgi and vacuoles. Phosphatidylserine transport to the mitochondria is ATP independent and exhibits no requirements for cytosolic factors. The phosphatidylethanolamine formed in the mitochondria is exported to the locus of the methyltransferases (principally the endoplasmic reticulum) and converted to phosphatidylcholine. The export of phosphatidylethanolamine requires ATP but not any other cytosolic factors and is not obligately coupled to methyltransferase activity. The above described lipid transport reactions also occur in permeabilized cells that have been disrupted by homogenization, indicating that the processes are extremely efficient and may be dependent upon stable structural elements between organelles.</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>BIOCHIMIE</subject><subject>Biological Transport</subject><subject>BIOQUIMICA</subject><subject>CEFALINAS</subject><subject>Cell Fractionation</subject><subject>Cell Membrane Permeability</subject><subject>CEPHALINE</subject><subject>Cytosol - metabolism</subject><subject>Kinetics</subject><subject>LECITHINE</subject><subject>LECITINAS</subject><subject>LIASAS</subject><subject>LIPOGENESE</subject><subject>LIPOGENESIS</subject><subject>LYASE</subject><subject>Methyltransferases - metabolism</subject><subject>Microsomes - metabolism</subject><subject>Mitochondria - metabolism</subject><subject>MITOCHONDRIE</subject><subject>MITOCONDRIA</subject><subject>ORGANITE CELLULAIRE</subject><subject>ORGANULOS CITOPLASMICOS</subject><subject>Phosphatidylcholines - biosynthesis</subject><subject>Phosphatidylcholines - isolation & purification</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phosphatidylethanolamines - biosynthesis</subject><subject>Phosphatidylethanolamines - isolation & purification</subject><subject>Phosphatidylethanolamines - metabolism</subject><subject>Phosphatidylserines - biosynthesis</subject><subject>Phosphatidylserines - isolation & purification</subject><subject>Phosphatidylserines - metabolism</subject><subject>Radioisotope Dilution Technique</subject><subject>RETICULO ENDOPLASMATICO</subject><subject>RETICULUM ENDOPLASMIQUE</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Serine - metabolism</subject><subject>Spheroplasts - metabolism</subject><subject>TRANSFERASAS</subject><subject>TRANSFERASE</subject><subject>Tritium</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV-L1DAUxYMo67j6LoKQBxEFOyZN0zS-LYN_FhYUZgR9CmlyO83SNt2ks8vsu-A38Pv5SczYwQdBDIQ8nHN-3NyD0GNKlpSI4vVlbZa5IEtOlrmsWHkHLSipWMY4_XIXLQjJaSZzXt1HD2K8JOkUkp6gk4ozwoRcoG_r_TC1EF3EerD4fJiCNtB1u04HvAl6iKMPE_YNPuvd4Lfd3kDwY-tjup0bnY3YDfgThB507Tp3CxavEiAeMomMv4KO0yu81sa0Ovg-ASJOELh20Wl48fP7j5cP0b1GdxEeHd9TtHn3drP6kF18fH--OrvIDGf5lHEOVd4QYW0OQtia27I2tq6lkVVJSgqSQyHKimgitaG6oKXRdSOJhJwyxk7R8xk7Bn-1gzip3sXDb_UAfheVEIKlrcr_GqkgrJKySEYyG03wMQZo1Bhcr8NeUaIODanUkEoNKU7U74ZS5OmRvat7sH8Cx0qS_mzWW7dtb1wAVTtvWuj_xjyZbY32Sm-Di-rzWgpasfww1ptZhLTMawdBReNgMGATz0zKevfvAX8BAqK3mQ</recordid><startdate>19951215</startdate><enddate>19951215</enddate><creator>Achleitner, Georg</creator><creator>Zweytick, Dagmar</creator><creator>Trotter, Pamela J.</creator><creator>Voelker, Dennis R.</creator><creator>Daum, Günther</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19951215</creationdate><title>Synthesis and Intracellular Transport of Aminoglycerophospholipids in Permeabilized Cells of the Yeast, Saccharomyces cerevisiae(∗)</title><author>Achleitner, Georg ; Zweytick, Dagmar ; Trotter, Pamela J. ; Voelker, Dennis R. ; Daum, Günther</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-55e82f07dd2e77db5d6bcdbb9c986061e95e47680a09ac1a416cabf909e21333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>BIOCHIMIE</topic><topic>Biological Transport</topic><topic>BIOQUIMICA</topic><topic>CEFALINAS</topic><topic>Cell Fractionation</topic><topic>Cell Membrane Permeability</topic><topic>CEPHALINE</topic><topic>Cytosol - metabolism</topic><topic>Kinetics</topic><topic>LECITHINE</topic><topic>LECITINAS</topic><topic>LIASAS</topic><topic>LIPOGENESE</topic><topic>LIPOGENESIS</topic><topic>LYASE</topic><topic>Methyltransferases - metabolism</topic><topic>Microsomes - metabolism</topic><topic>Mitochondria - metabolism</topic><topic>MITOCHONDRIE</topic><topic>MITOCONDRIA</topic><topic>ORGANITE CELLULAIRE</topic><topic>ORGANULOS CITOPLASMICOS</topic><topic>Phosphatidylcholines - biosynthesis</topic><topic>Phosphatidylcholines - isolation & purification</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phosphatidylethanolamines - biosynthesis</topic><topic>Phosphatidylethanolamines - isolation & purification</topic><topic>Phosphatidylethanolamines - metabolism</topic><topic>Phosphatidylserines - biosynthesis</topic><topic>Phosphatidylserines - isolation & purification</topic><topic>Phosphatidylserines - metabolism</topic><topic>Radioisotope Dilution Technique</topic><topic>RETICULO ENDOPLASMATICO</topic><topic>RETICULUM ENDOPLASMIQUE</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Serine - metabolism</topic><topic>Spheroplasts - metabolism</topic><topic>TRANSFERASAS</topic><topic>TRANSFERASE</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Achleitner, Georg</creatorcontrib><creatorcontrib>Zweytick, Dagmar</creatorcontrib><creatorcontrib>Trotter, Pamela J.</creatorcontrib><creatorcontrib>Voelker, Dennis R.</creatorcontrib><creatorcontrib>Daum, Günther</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Achleitner, Georg</au><au>Zweytick, Dagmar</au><au>Trotter, Pamela J.</au><au>Voelker, Dennis R.</au><au>Daum, Günther</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and Intracellular Transport of Aminoglycerophospholipids in Permeabilized Cells of the Yeast, Saccharomyces cerevisiae(∗)</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1995-12-15</date><risdate>1995</risdate><volume>270</volume><issue>50</issue><spage>29836</spage><epage>29842</epage><pages>29836-29842</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The sequence of biosynthetic steps from phosphatidylserine to phosphatidylethanolamine (via decarboxylation) and then phosphatidylcholine (via methylation) is linked to the intracellular transport of these aminoglycerophospholipids. Using a [3H]serine precursor and permeabilized yeast cells, it is possible to follow the synthesis of each of the aminoglycerophospholipids and examine the requirements for their interorganelle transport. This experimental approach reveals that in permeabilized cells newly synthesized phosphatidylserine is readily translocated to the locus of phosphatidylserine decarboxylase 1 in the mitochondria but not to the locus of phosphatidylserine decarboxylase 2 in the Golgi and vacuoles. Phosphatidylserine transport to the mitochondria is ATP independent and exhibits no requirements for cytosolic factors. The phosphatidylethanolamine formed in the mitochondria is exported to the locus of the methyltransferases (principally the endoplasmic reticulum) and converted to phosphatidylcholine. The export of phosphatidylethanolamine requires ATP but not any other cytosolic factors and is not obligately coupled to methyltransferase activity. The above described lipid transport reactions also occur in permeabilized cells that have been disrupted by homogenization, indicating that the processes are extremely efficient and may be dependent upon stable structural elements between organelles.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8530379</pmid><doi>10.1074/jbc.270.50.29836</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1995-12, Vol.270 (50), p.29836-29842 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE BIOCHIMIE Biological Transport BIOQUIMICA CEFALINAS Cell Fractionation Cell Membrane Permeability CEPHALINE Cytosol - metabolism Kinetics LECITHINE LECITINAS LIASAS LIPOGENESE LIPOGENESIS LYASE Methyltransferases - metabolism Microsomes - metabolism Mitochondria - metabolism MITOCHONDRIE MITOCONDRIA ORGANITE CELLULAIRE ORGANULOS CITOPLASMICOS Phosphatidylcholines - biosynthesis Phosphatidylcholines - isolation & purification Phosphatidylcholines - metabolism Phosphatidylethanolamines - biosynthesis Phosphatidylethanolamines - isolation & purification Phosphatidylethanolamines - metabolism Phosphatidylserines - biosynthesis Phosphatidylserines - isolation & purification Phosphatidylserines - metabolism Radioisotope Dilution Technique RETICULO ENDOPLASMATICO RETICULUM ENDOPLASMIQUE SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - metabolism Serine - metabolism Spheroplasts - metabolism TRANSFERASAS TRANSFERASE Tritium |
| title | Synthesis and Intracellular Transport of Aminoglycerophospholipids in Permeabilized Cells of the Yeast, Saccharomyces cerevisiae(∗) |
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