Suppression of HIV replication in human monocyte-derived macrophages induced by granulocyte/macrophage colony-stimulating factor
Susceptibility to HIV infection was examined in macrophages differentiated from human monocytes by macrophage colony-stimulating factor (M-CSF) or granulocyte/macrophage colony-stimulating factor (GM-CSF). The replication of macrophage-tropic human immunodeficiency virus type-1 (HIV-1), which was de...
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Veröffentlicht in: | AIDS research and human retroviruses 1995-09, Vol.11 (9), p.1031-1038 |
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description | Susceptibility to HIV infection was examined in macrophages differentiated from human monocytes by macrophage colony-stimulating factor (M-CSF) or granulocyte/macrophage colony-stimulating factor (GM-CSF). The replication of macrophage-tropic human immunodeficiency virus type-1 (HIV-1), which was determined by reverse transcriptase (RT) activity, was significantly suppressed in macrophages induced by GM-CSF (GM-type macrophages) but not in those induced by M-CSF (M-type macrophages). Multinucleated giant cells were formed only in M-type macrophages after HIV infection. However, the expression of CD4 molecules on the surface of both types of macrophages was similar and the proviral DNA was detectable in cell lysates of both macrophages, although the amount of proviral DNA in M-type macrophages was higher than that in GM-type macrophages. Many steps have been defined in HIV infection and replication, such as adsorption of HIV to the cell surface, internalization of the viral core into the cytoplasm, uncoating of viral RNA, reverse transcription and integration of proviral DNA into cellular DNA, transcription and translation of proviral DNA, assembly of viral components, and budding of virus particles. Our findings suggested that the suppression of HIV-1 replication in macrophages induced by GM-CSF is mainly due to a disturbance at certain steps of replication after synthesis of the proviral DNA. Thus, the suppression of HIV replication in GM-type macrophages may provide a model of the latency of HIV infection in vivo. |
doi_str_mv | 10.1089/aid.1995.11.1031 |
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The replication of macrophage-tropic human immunodeficiency virus type-1 (HIV-1), which was determined by reverse transcriptase (RT) activity, was significantly suppressed in macrophages induced by GM-CSF (GM-type macrophages) but not in those induced by M-CSF (M-type macrophages). Multinucleated giant cells were formed only in M-type macrophages after HIV infection. However, the expression of CD4 molecules on the surface of both types of macrophages was similar and the proviral DNA was detectable in cell lysates of both macrophages, although the amount of proviral DNA in M-type macrophages was higher than that in GM-type macrophages. Many steps have been defined in HIV infection and replication, such as adsorption of HIV to the cell surface, internalization of the viral core into the cytoplasm, uncoating of viral RNA, reverse transcription and integration of proviral DNA into cellular DNA, transcription and translation of proviral DNA, assembly of viral components, and budding of virus particles. Our findings suggested that the suppression of HIV-1 replication in macrophages induced by GM-CSF is mainly due to a disturbance at certain steps of replication after synthesis of the proviral DNA. Thus, the suppression of HIV replication in GM-type macrophages may provide a model of the latency of HIV infection in vivo.</description><identifier>ISSN: 0889-2229</identifier><identifier>EISSN: 1931-8405</identifier><identifier>DOI: 10.1089/aid.1995.11.1031</identifier><identifier>PMID: 8554900</identifier><identifier>CODEN: ARHRE7</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>AIDS/HIV ; Antigens, Surface - metabolism ; Base Sequence ; Biological and medical sciences ; CD4 Antigens - metabolism ; Cell Differentiation ; Cells, Cultured ; DNA Primers - genetics ; DNA, Viral - genetics ; DNA, Viral - metabolism ; Fundamental and applied biological sciences. Psychology ; Genes, gag ; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology ; HIV Infections - pathology ; HIV-1 - drug effects ; HIV-1 - genetics ; HIV-1 - physiology ; human immunodeficiency virus 1 ; Humans ; Macrophage Colony-Stimulating Factor - pharmacology ; Macrophages - cytology ; Macrophages - drug effects ; Macrophages - virology ; Microbiology ; Microscopy, Electron ; Molecular Sequence Data ; Monocytes - cytology ; Monocytes - drug effects ; Proviruses - drug effects ; Proviruses - genetics ; Proviruses - physiology ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Virology ; Virus Replication - drug effects</subject><ispartof>AIDS research and human retroviruses, 1995-09, Vol.11 (9), p.1031-1038</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-715916e4a7337fefcba99c96004b711f7a7db683d927acf944ee75c781845b373</citedby><cites>FETCH-LOGICAL-c420t-715916e4a7337fefcba99c96004b711f7a7db683d927acf944ee75c781845b373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3042,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3665281$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8554900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MATSUDA, S</creatorcontrib><creatorcontrib>AKAGAWA, K</creatorcontrib><creatorcontrib>HONDA, M</creatorcontrib><creatorcontrib>YOKOTA, Y</creatorcontrib><creatorcontrib>TAKEBE, Y</creatorcontrib><creatorcontrib>TAKEMORI, T</creatorcontrib><title>Suppression of HIV replication in human monocyte-derived macrophages induced by granulocyte/macrophage colony-stimulating factor</title><title>AIDS research and human retroviruses</title><addtitle>AIDS Res Hum Retroviruses</addtitle><description>Susceptibility to HIV infection was examined in macrophages differentiated from human monocytes by macrophage colony-stimulating factor (M-CSF) or granulocyte/macrophage colony-stimulating factor (GM-CSF). The replication of macrophage-tropic human immunodeficiency virus type-1 (HIV-1), which was determined by reverse transcriptase (RT) activity, was significantly suppressed in macrophages induced by GM-CSF (GM-type macrophages) but not in those induced by M-CSF (M-type macrophages). Multinucleated giant cells were formed only in M-type macrophages after HIV infection. However, the expression of CD4 molecules on the surface of both types of macrophages was similar and the proviral DNA was detectable in cell lysates of both macrophages, although the amount of proviral DNA in M-type macrophages was higher than that in GM-type macrophages. Many steps have been defined in HIV infection and replication, such as adsorption of HIV to the cell surface, internalization of the viral core into the cytoplasm, uncoating of viral RNA, reverse transcription and integration of proviral DNA into cellular DNA, transcription and translation of proviral DNA, assembly of viral components, and budding of virus particles. Our findings suggested that the suppression of HIV-1 replication in macrophages induced by GM-CSF is mainly due to a disturbance at certain steps of replication after synthesis of the proviral DNA. Thus, the suppression of HIV replication in GM-type macrophages may provide a model of the latency of HIV infection in vivo.</description><subject>AIDS/HIV</subject><subject>Antigens, Surface - metabolism</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CD4 Antigens - metabolism</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>DNA Primers - genetics</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, gag</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>HIV Infections - pathology</subject><subject>HIV-1 - drug effects</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - physiology</subject><subject>human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>Macrophages - cytology</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - virology</subject><subject>Microbiology</subject><subject>Microscopy, Electron</subject><subject>Molecular Sequence Data</subject><subject>Monocytes - cytology</subject><subject>Monocytes - drug effects</subject><subject>Proviruses - drug effects</subject><subject>Proviruses - genetics</subject><subject>Proviruses - physiology</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Virology</subject><subject>Virus Replication - drug effects</subject><issn>0889-2229</issn><issn>1931-8405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtrHDEURkVIsNdO-jQGFSHdrHX1GEmlMYltMKTIoxUajbRWmBmNpZnAdvnp0dqLXaYSfPfcD3QPQh-BbIEofWljvwWtxRagBgzeoA1oBo3iRLxFG6KUbiil-hSdlfKbEKIpFSfoRAnBNSEb9Pf7Os_ZlxLThFPAt3e_cPbzEJ1dDlGc8MM62gmPaUpuv_im9zn-8T0erctpfrA7XyrVr65m3R7vsp3W4Qm9fEWwS0Oa9k1Z4rgOtXra4WDdkvJ79C7YofgPx_cc_fz65cf1bXP_7ebu-uq-cZySpZEgNLSeW8mYDD64zmrtdEsI7yRAkFb2XatYr6m0LmjOvZfCSQWKi45Jdo4-P_fOOT2uvixmjMX5YbCTT2sxUkqquG7_C0KrWk04ryB5BusnS8k-mDnH0ea9AWIOdky1Yw52DIA52KkrF8futRt9_7Jw1FHnn45zW5wdQr2li-UFY20rqAL2D3Dzmqk</recordid><startdate>19950901</startdate><enddate>19950901</enddate><creator>MATSUDA, S</creator><creator>AKAGAWA, K</creator><creator>HONDA, M</creator><creator>YOKOTA, Y</creator><creator>TAKEBE, Y</creator><creator>TAKEMORI, T</creator><general>Liebert</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19950901</creationdate><title>Suppression of HIV replication in human monocyte-derived macrophages induced by granulocyte/macrophage colony-stimulating factor</title><author>MATSUDA, S ; AKAGAWA, K ; HONDA, M ; YOKOTA, Y ; TAKEBE, Y ; TAKEMORI, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-715916e4a7337fefcba99c96004b711f7a7db683d927acf944ee75c781845b373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AIDS/HIV</topic><topic>Antigens, Surface - metabolism</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CD4 Antigens - metabolism</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>DNA Primers - genetics</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, gag</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>HIV Infections - pathology</topic><topic>HIV-1 - drug effects</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - physiology</topic><topic>human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>Macrophages - cytology</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - virology</topic><topic>Microbiology</topic><topic>Microscopy, Electron</topic><topic>Molecular Sequence Data</topic><topic>Monocytes - cytology</topic><topic>Monocytes - drug effects</topic><topic>Proviruses - drug effects</topic><topic>Proviruses - genetics</topic><topic>Proviruses - physiology</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Virology</topic><topic>Virus Replication - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MATSUDA, S</creatorcontrib><creatorcontrib>AKAGAWA, K</creatorcontrib><creatorcontrib>HONDA, M</creatorcontrib><creatorcontrib>YOKOTA, Y</creatorcontrib><creatorcontrib>TAKEBE, Y</creatorcontrib><creatorcontrib>TAKEMORI, T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS research and human retroviruses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MATSUDA, S</au><au>AKAGAWA, K</au><au>HONDA, M</au><au>YOKOTA, Y</au><au>TAKEBE, Y</au><au>TAKEMORI, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression of HIV replication in human monocyte-derived macrophages induced by granulocyte/macrophage colony-stimulating factor</atitle><jtitle>AIDS research and human retroviruses</jtitle><addtitle>AIDS Res Hum Retroviruses</addtitle><date>1995-09-01</date><risdate>1995</risdate><volume>11</volume><issue>9</issue><spage>1031</spage><epage>1038</epage><pages>1031-1038</pages><issn>0889-2229</issn><eissn>1931-8405</eissn><coden>ARHRE7</coden><abstract>Susceptibility to HIV infection was examined in macrophages differentiated from human monocytes by macrophage colony-stimulating factor (M-CSF) or granulocyte/macrophage colony-stimulating factor (GM-CSF). The replication of macrophage-tropic human immunodeficiency virus type-1 (HIV-1), which was determined by reverse transcriptase (RT) activity, was significantly suppressed in macrophages induced by GM-CSF (GM-type macrophages) but not in those induced by M-CSF (M-type macrophages). Multinucleated giant cells were formed only in M-type macrophages after HIV infection. However, the expression of CD4 molecules on the surface of both types of macrophages was similar and the proviral DNA was detectable in cell lysates of both macrophages, although the amount of proviral DNA in M-type macrophages was higher than that in GM-type macrophages. Many steps have been defined in HIV infection and replication, such as adsorption of HIV to the cell surface, internalization of the viral core into the cytoplasm, uncoating of viral RNA, reverse transcription and integration of proviral DNA into cellular DNA, transcription and translation of proviral DNA, assembly of viral components, and budding of virus particles. Our findings suggested that the suppression of HIV-1 replication in macrophages induced by GM-CSF is mainly due to a disturbance at certain steps of replication after synthesis of the proviral DNA. Thus, the suppression of HIV replication in GM-type macrophages may provide a model of the latency of HIV infection in vivo.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>8554900</pmid><doi>10.1089/aid.1995.11.1031</doi><tpages>8</tpages></addata></record> |
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subjects | AIDS/HIV Antigens, Surface - metabolism Base Sequence Biological and medical sciences CD4 Antigens - metabolism Cell Differentiation Cells, Cultured DNA Primers - genetics DNA, Viral - genetics DNA, Viral - metabolism Fundamental and applied biological sciences. Psychology Genes, gag Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology HIV Infections - pathology HIV-1 - drug effects HIV-1 - genetics HIV-1 - physiology human immunodeficiency virus 1 Humans Macrophage Colony-Stimulating Factor - pharmacology Macrophages - cytology Macrophages - drug effects Macrophages - virology Microbiology Microscopy, Electron Molecular Sequence Data Monocytes - cytology Monocytes - drug effects Proviruses - drug effects Proviruses - genetics Proviruses - physiology Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Virology Virus Replication - drug effects |
title | Suppression of HIV replication in human monocyte-derived macrophages induced by granulocyte/macrophage colony-stimulating factor |
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