PCR expression analysis of the estrogen-inducible gene BCEI in gastrointestinal and other human tumors
A polymerase chain reaction (PCR) assay was developed to test for tumor cell specific expression of the BCEI gene. This new marker gene, reported at first for human breast cancer, was found specifically active in various gastrointestinal carcinomas by previously applying immunohistochemistry and RNA...
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| Veröffentlicht in: | Disease markers 1994-10, Vol.12 (1), p.63-69 |
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| creator | Wundrack, I Müllenbach, R Welter, C Seitz, G Blin, N |
| description | A polymerase chain reaction (PCR) assay was developed to test for tumor cell specific expression of the BCEI gene. This new marker gene, reported at first for human breast cancer, was found specifically active in various gastrointestinal carcinomas by previously applying immunohistochemistry and RNA (Northern blot) analysis. Presently, by using reverse transcription-PCR analysis, a series of primary tumor tissues and established tumor cell lines were tested for BCEI transcription. This approach was compared to immunostaining achieved by an antibody directed against the BCEI gene's product. The result demonstrate the superior sensitivity of PCR by indicating the gene's expression in cases where immunohistochemical testing remained negative. |
| doi_str_mv | 10.1155/1994/542396 |
| format | Article |
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The result demonstrate the superior sensitivity of PCR by indicating the gene's expression in cases where immunohistochemical testing remained negative.</description><subject>Base Sequence</subject><subject>BCEI gene</subject><subject>Blotting, Northern</subject><subject>Breast Neoplasms - genetics</subject><subject>carcinoma</subject><subject>colorectal</subject><subject>Estrogens - pharmacology</subject><subject>Gastrointestinal Neoplasms - genetics</subject><subject>gastrointestinal tract diseases</subject><subject>gene expression</subject><subject>Genetic Markers</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>man</subject><subject>Molecular Sequence Data</subject><subject>Neoplasm Proteins - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Proteins</subject><subject>RNA-Directed DNA Polymerase</subject><subject>Trefoil Factor-1</subject><subject>Tumor Suppressor Proteins</subject><issn>0278-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LwzAYhnNQ5pyePAs5eavLj2Zpjlo2HQwU2b0k7Zct0qY1acH990bc3dPHC8_78PEidEfJI6VCLKlS-VLkjKvVBZoTJouMsJxcoesYPwmhTOVqhmZScEpZMUf2vfzA8D0EiNH1Hmuv21N0EfcWj0fAEMfQH8BnzjdT7UwLOCXAz-V6i53HB_0LOD8m0KVuEjS4T82Aj1OnPR6nrg_xBl1a3Ua4Pd8F2m_W-_I12729bMunXTYwLsesFlIxw4QhstFC89qA0I1trMlhxRqdm4IBLagixrBaUVBGSS4sz60iVvMFevjTDqH_mtJLVediDW2rPfRTrKSUaQxG_gXpKnmVoAm8P4OT6aCphuA6HU7VeUD-A4A1cRg</recordid><startdate>19941001</startdate><enddate>19941001</enddate><creator>Wundrack, I</creator><creator>Müllenbach, R</creator><creator>Welter, C</creator><creator>Seitz, G</creator><creator>Blin, N</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T3</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19941001</creationdate><title>PCR expression analysis of the estrogen-inducible gene BCEI in gastrointestinal and other human tumors</title><author>Wundrack, I ; Müllenbach, R ; Welter, C ; Seitz, G ; Blin, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p237t-c5792b25b07da5a3cbe5adfdfb4e62da4b82e18190bb2c91e9b9735f34f90fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Base Sequence</topic><topic>BCEI gene</topic><topic>Blotting, Northern</topic><topic>Breast Neoplasms - genetics</topic><topic>carcinoma</topic><topic>colorectal</topic><topic>Estrogens - pharmacology</topic><topic>Gastrointestinal Neoplasms - genetics</topic><topic>gastrointestinal tract diseases</topic><topic>gene expression</topic><topic>Genetic Markers</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>man</topic><topic>Molecular Sequence Data</topic><topic>Neoplasm Proteins - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Proteins</topic><topic>RNA-Directed DNA Polymerase</topic><topic>Trefoil Factor-1</topic><topic>Tumor Suppressor Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wundrack, I</creatorcontrib><creatorcontrib>Müllenbach, R</creatorcontrib><creatorcontrib>Welter, C</creatorcontrib><creatorcontrib>Seitz, G</creatorcontrib><creatorcontrib>Blin, N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Human Genome Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wundrack, I</au><au>Müllenbach, R</au><au>Welter, C</au><au>Seitz, G</au><au>Blin, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PCR expression analysis of the estrogen-inducible gene BCEI in gastrointestinal and other human tumors</atitle><jtitle>Disease markers</jtitle><addtitle>Dis Markers</addtitle><date>1994-10-01</date><risdate>1994</risdate><volume>12</volume><issue>1</issue><spage>63</spage><epage>69</epage><pages>63-69</pages><issn>0278-0240</issn><abstract>A polymerase chain reaction (PCR) assay was developed to test for tumor cell specific expression of the BCEI gene. 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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Base Sequence BCEI gene Blotting, Northern Breast Neoplasms - genetics carcinoma colorectal Estrogens - pharmacology Gastrointestinal Neoplasms - genetics gastrointestinal tract diseases gene expression Genetic Markers Humans Immunohistochemistry man Molecular Sequence Data Neoplasm Proteins - genetics Polymerase Chain Reaction Proteins RNA-Directed DNA Polymerase Trefoil Factor-1 Tumor Suppressor Proteins |
| title | PCR expression analysis of the estrogen-inducible gene BCEI in gastrointestinal and other human tumors |
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