cDNA Sequence Analysis of the Human Brain Insulin Receptor
Brain tissue mRNA was amplified using polymerase chain reaction (PCR) with eight overlapping sets of primers that span the cDNA coding sequence for the human placental insulin receptor. Only the A isoform (lacking exon 11) of the receptor was detected. No difference was found in the predicted amino...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1995-12, Vol.217 (1), p.304-312 |
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| creator | Kenner, K.A. Kusari, J. Heidenreich, K.A. |
| description | Brain tissue mRNA was amplified using polymerase chain reaction (PCR) with eight overlapping sets of primers that span the cDNA coding sequence for the human placental insulin receptor. Only the A isoform (lacking exon 11) of the receptor was detected. No difference was found in the predicted amino acid sequence of brain derived insulin receptor cDNA compared with the receptor from human placenta. A silent polymorphism was detected at nucleotide position 1698 (amino acid 523), confirming that mRNA corresponding to both alleles of the human brain receptor was sequenced. Our findings indicate that the unique glycosylation properties of brain insulin receptors do not stem from differences in primary structure, but rather are due to tissue-specific differences in post-translational processing. |
| doi_str_mv | 10.1006/bbrc.1995.2778 |
| format | Article |
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Only the A isoform (lacking exon 11) of the receptor was detected. No difference was found in the predicted amino acid sequence of brain derived insulin receptor cDNA compared with the receptor from human placenta. A silent polymorphism was detected at nucleotide position 1698 (amino acid 523), confirming that mRNA corresponding to both alleles of the human brain receptor was sequenced. Our findings indicate that the unique glycosylation properties of brain insulin receptors do not stem from differences in primary structure, but rather are due to tissue-specific differences in post-translational processing.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1995.2778</identifier><identifier>PMID: 8526927</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Base Sequence ; Brain - metabolism ; DNA Primers - genetics ; DNA, Complementary - genetics ; Female ; Humans ; Molecular Sequence Data ; Placenta - metabolism ; Polymerase Chain Reaction ; Pregnancy ; Protein Conformation ; Protein Processing, Post-Translational ; Receptor, Insulin - chemistry ; Receptor, Insulin - genetics ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; Tissue Distribution</subject><ispartof>Biochemical and biophysical research communications, 1995-12, Vol.217 (1), p.304-312</ispartof><rights>1995 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-5dd393482c94a2fa8ab13a9613030d600a39fc7eaf616e7b9effcc3528ea4d9d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1995.2778$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8526927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kenner, K.A.</creatorcontrib><creatorcontrib>Kusari, J.</creatorcontrib><creatorcontrib>Heidenreich, K.A.</creatorcontrib><title>cDNA Sequence Analysis of the Human Brain Insulin Receptor</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Brain tissue mRNA was amplified using polymerase chain reaction (PCR) with eight overlapping sets of primers that span the cDNA coding sequence for the human placental insulin receptor. Only the A isoform (lacking exon 11) of the receptor was detected. No difference was found in the predicted amino acid sequence of brain derived insulin receptor cDNA compared with the receptor from human placenta. A silent polymorphism was detected at nucleotide position 1698 (amino acid 523), confirming that mRNA corresponding to both alleles of the human brain receptor was sequenced. Our findings indicate that the unique glycosylation properties of brain insulin receptors do not stem from differences in primary structure, but rather are due to tissue-specific differences in post-translational processing.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Brain - metabolism</subject><subject>DNA Primers - genetics</subject><subject>DNA, Complementary - genetics</subject><subject>Female</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Placenta - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><subject>Protein Conformation</subject><subject>Protein Processing, Post-Translational</subject><subject>Receptor, Insulin - chemistry</subject><subject>Receptor, Insulin - genetics</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Tissue Distribution</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDtPwzAURi0EKqWwsiFlYkvwI7FjtlJelSqQeEhslmNfC6M0KXaC1H9PolZsTHf4zv1070HonOCMYMyvqiqYjEhZZFSI8gBNCZY4pQTnh2iKByKlknwco5MYvzAmJOdygiZlQbmkYoquze3TPHmF7x4aA8m80fU2-pi0Luk-IXns17pJboL2TbJsYl8P8wUMbLo2nKIjp-sIZ_s5Q-_3d2-Lx3T1_LBczFepYUx2aWEtkywvqZG5pk6XuiJMS04YZthyjDWTzgjQjhMOopLgnDGsoCXo3ErLZuhy17sJ7XBm7NTaRwN1rRto-6iEEJQKxgcw24EmtDEGcGoT_FqHrSJYjbLUKEuNstQoa1i42Df31RrsH763M-TlLofhvR8PQUXjR0_WBzCdsq3_r_oXrsx3xQ</recordid><startdate>19951205</startdate><enddate>19951205</enddate><creator>Kenner, K.A.</creator><creator>Kusari, J.</creator><creator>Heidenreich, K.A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951205</creationdate><title>cDNA Sequence Analysis of the Human Brain Insulin Receptor</title><author>Kenner, K.A. ; Kusari, J. ; Heidenreich, K.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-5dd393482c94a2fa8ab13a9613030d600a39fc7eaf616e7b9effcc3528ea4d9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Brain - metabolism</topic><topic>DNA Primers - genetics</topic><topic>DNA, Complementary - genetics</topic><topic>Female</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Placenta - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Pregnancy</topic><topic>Protein Conformation</topic><topic>Protein Processing, Post-Translational</topic><topic>Receptor, Insulin - chemistry</topic><topic>Receptor, Insulin - genetics</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kenner, K.A.</creatorcontrib><creatorcontrib>Kusari, J.</creatorcontrib><creatorcontrib>Heidenreich, K.A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kenner, K.A.</au><au>Kusari, J.</au><au>Heidenreich, K.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA Sequence Analysis of the Human Brain Insulin Receptor</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1995-12-05</date><risdate>1995</risdate><volume>217</volume><issue>1</issue><spage>304</spage><epage>312</epage><pages>304-312</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Brain tissue mRNA was amplified using polymerase chain reaction (PCR) with eight overlapping sets of primers that span the cDNA coding sequence for the human placental insulin receptor. Only the A isoform (lacking exon 11) of the receptor was detected. No difference was found in the predicted amino acid sequence of brain derived insulin receptor cDNA compared with the receptor from human placenta. A silent polymorphism was detected at nucleotide position 1698 (amino acid 523), confirming that mRNA corresponding to both alleles of the human brain receptor was sequenced. Our findings indicate that the unique glycosylation properties of brain insulin receptors do not stem from differences in primary structure, but rather are due to tissue-specific differences in post-translational processing.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8526927</pmid><doi>10.1006/bbrc.1995.2778</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1995-12, Vol.217 (1), p.304-312 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Base Sequence Brain - metabolism DNA Primers - genetics DNA, Complementary - genetics Female Humans Molecular Sequence Data Placenta - metabolism Polymerase Chain Reaction Pregnancy Protein Conformation Protein Processing, Post-Translational Receptor, Insulin - chemistry Receptor, Insulin - genetics Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Tissue Distribution |
| title | cDNA Sequence Analysis of the Human Brain Insulin Receptor |
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