Molecular analysis of human T-cell lymphotropic virus type II from Wayuu Indians of Colombia demonstrates two subtypes of HTLV-IIb
Studies of the genetic heterogeneity of human T-cell lymphotropic virus type II (HTLV-II) have revealed the presence of two genetic subtypes, termed HTLV-IIa and HTLV-IIb. The HTLV-IIb subtype encodes an immunodominant epitope present at the C-terminus of the extended Tax protein and, by using an LT...
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| Veröffentlicht in: | Virus genes 1995, Vol.10 (2), p.153-162 |
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| creator | Switzer, W M Owen, S M Pieniazek, D A Nerurkar, V R Duenas-Barajas, E Heneine, W Lal, R B |
| description | Studies of the genetic heterogeneity of human T-cell lymphotropic virus type II (HTLV-II) have revealed the presence of two genetic subtypes, termed HTLV-IIa and HTLV-IIb. The HTLV-IIb subtype encodes an immunodominant epitope present at the C-terminus of the extended Tax protein and, by using an LTR-based, restriction fragment-length polymorphism (RFLP) assay, can be further classified into IIb60-IIb5, with HTLV-IIb1 (Central Amerindian-like) and HTLV-IIb5 (North Amerindian-like) being characteristic subtypes for Native American Indians. To determine the antigenic and genetic heterogeneity among HTLV-II-infected South Amerindians, we used a Tax synthetic peptide immunoassay on serum, and RFLP and phylogenetic analysis on LTR sequences amplified from genomic DNA from four Wayuu Indians of Colombia. The Wayuu specimens displayed seroreactivity to the immunodominant epitope located in the extended Tax region, as predicted, and demonstrated genetic heterogeneity by the presence of both the IIB1 (Wyu1, Zuc31) and IIb5 (Wyu2, Zuc42) subtypes sequences within separate phylogroups represented by the Guaymi Indian (IIb1) and North Amerindian (IIb5) sequences, respectively. Sequence analysis showed that major LTR regulatory motifs and the cis-acting repressive elements in the LTR RNA secondary structure were relatively conserved in both Wayuu subtypes, but the predicted secondary structure of the rex response stem loop in the Wyu2 (IIb5) LTR sequence was 45 nucleotides (nt) and 95 nt longer than that observed in the Wyu1 (IIb1) and G12.1 (IIb1) LTR sequences, respectively. These results extend our knowledge of the genetic heterogeneity of HTLV-II in South Amerindians. |
| doi_str_mv | 10.1007/BF01702596 |
| format | Article |
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The HTLV-IIb subtype encodes an immunodominant epitope present at the C-terminus of the extended Tax protein and, by using an LTR-based, restriction fragment-length polymorphism (RFLP) assay, can be further classified into IIb60-IIb5, with HTLV-IIb1 (Central Amerindian-like) and HTLV-IIb5 (North Amerindian-like) being characteristic subtypes for Native American Indians. To determine the antigenic and genetic heterogeneity among HTLV-II-infected South Amerindians, we used a Tax synthetic peptide immunoassay on serum, and RFLP and phylogenetic analysis on LTR sequences amplified from genomic DNA from four Wayuu Indians of Colombia. The Wayuu specimens displayed seroreactivity to the immunodominant epitope located in the extended Tax region, as predicted, and demonstrated genetic heterogeneity by the presence of both the IIB1 (Wyu1, Zuc31) and IIb5 (Wyu2, Zuc42) subtypes sequences within separate phylogroups represented by the Guaymi Indian (IIb1) and North Amerindian (IIb5) sequences, respectively. Sequence analysis showed that major LTR regulatory motifs and the cis-acting repressive elements in the LTR RNA secondary structure were relatively conserved in both Wayuu subtypes, but the predicted secondary structure of the rex response stem loop in the Wyu2 (IIb5) LTR sequence was 45 nucleotides (nt) and 95 nt longer than that observed in the Wyu1 (IIb1) and G12.1 (IIb1) LTR sequences, respectively. These results extend our knowledge of the genetic heterogeneity of HTLV-II in South Amerindians.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/BF01702596</identifier><identifier>PMID: 8560775</identifier><language>eng</language><publisher>United States</publisher><subject>AIDS/HIV ; Amino Acid Sequence ; Antigens, Viral - immunology ; Base Sequence ; Cell Line, Transformed ; Colombia - epidemiology ; DNA, Viral - chemistry ; DNA, Viral - genetics ; Gene Products, tax - genetics ; Genes, Viral ; Genetic Heterogeneity ; HTLV-II Infections - epidemiology ; HTLV-II Infections - virology ; Human T-lymphotropic virus 2 - classification ; Human T-lymphotropic virus 2 - genetics ; Human T-lymphotropic virus 2 - immunology ; Human T-lymphotropic virus 2 - isolation & purification ; Humans ; Indians, South American ; Molecular Sequence Data ; Nucleic Acid Conformation ; Phylogeny ; Repetitive Sequences, Nucleic Acid ; Serotyping</subject><ispartof>Virus genes, 1995, Vol.10 (2), p.153-162</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c228t-d9e4dd5bc6831a7c837f10588c0227584dfcefc5e85cc9ed2dbcd2a137fe7ba83</citedby><cites>FETCH-LOGICAL-c228t-d9e4dd5bc6831a7c837f10588c0227584dfcefc5e85cc9ed2dbcd2a137fe7ba83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8560775$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Switzer, W M</creatorcontrib><creatorcontrib>Owen, S M</creatorcontrib><creatorcontrib>Pieniazek, D A</creatorcontrib><creatorcontrib>Nerurkar, V R</creatorcontrib><creatorcontrib>Duenas-Barajas, E</creatorcontrib><creatorcontrib>Heneine, W</creatorcontrib><creatorcontrib>Lal, R B</creatorcontrib><title>Molecular analysis of human T-cell lymphotropic virus type II from Wayuu Indians of Colombia demonstrates two subtypes of HTLV-IIb</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><description>Studies of the genetic heterogeneity of human T-cell lymphotropic virus type II (HTLV-II) have revealed the presence of two genetic subtypes, termed HTLV-IIa and HTLV-IIb. The HTLV-IIb subtype encodes an immunodominant epitope present at the C-terminus of the extended Tax protein and, by using an LTR-based, restriction fragment-length polymorphism (RFLP) assay, can be further classified into IIb60-IIb5, with HTLV-IIb1 (Central Amerindian-like) and HTLV-IIb5 (North Amerindian-like) being characteristic subtypes for Native American Indians. To determine the antigenic and genetic heterogeneity among HTLV-II-infected South Amerindians, we used a Tax synthetic peptide immunoassay on serum, and RFLP and phylogenetic analysis on LTR sequences amplified from genomic DNA from four Wayuu Indians of Colombia. The Wayuu specimens displayed seroreactivity to the immunodominant epitope located in the extended Tax region, as predicted, and demonstrated genetic heterogeneity by the presence of both the IIB1 (Wyu1, Zuc31) and IIb5 (Wyu2, Zuc42) subtypes sequences within separate phylogroups represented by the Guaymi Indian (IIb1) and North Amerindian (IIb5) sequences, respectively. Sequence analysis showed that major LTR regulatory motifs and the cis-acting repressive elements in the LTR RNA secondary structure were relatively conserved in both Wayuu subtypes, but the predicted secondary structure of the rex response stem loop in the Wyu2 (IIb5) LTR sequence was 45 nucleotides (nt) and 95 nt longer than that observed in the Wyu1 (IIb1) and G12.1 (IIb1) LTR sequences, respectively. These results extend our knowledge of the genetic heterogeneity of HTLV-II in South Amerindians.</description><subject>AIDS/HIV</subject><subject>Amino Acid Sequence</subject><subject>Antigens, Viral - immunology</subject><subject>Base Sequence</subject><subject>Cell Line, Transformed</subject><subject>Colombia - epidemiology</subject><subject>DNA, Viral - chemistry</subject><subject>DNA, Viral - genetics</subject><subject>Gene Products, tax - genetics</subject><subject>Genes, Viral</subject><subject>Genetic Heterogeneity</subject><subject>HTLV-II Infections - epidemiology</subject><subject>HTLV-II Infections - virology</subject><subject>Human T-lymphotropic virus 2 - classification</subject><subject>Human T-lymphotropic virus 2 - genetics</subject><subject>Human T-lymphotropic virus 2 - immunology</subject><subject>Human T-lymphotropic virus 2 - isolation & purification</subject><subject>Humans</subject><subject>Indians, South American</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Phylogeny</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Serotyping</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1LwzAYBvAgypzTi3chJw9CNUmbJT3qcK4w8TI_biVNUlZJm5o0Sq_-5XYf6NHTCy-_54WXB4BzjK4xQuzmbo4wQ4Sm0wMwxpSRKE2Tt0MwRilBEafT9BiceP-OEOKcJCMwGnaIMToG34_WaBmMcFA0wvS-8tCWcB1q0cBVJLUx0PR1u7ads20l4Wflgodd32qYZbB0toavog8BZo2qRLNNz6yxdVEJqHRtG9850ekh82WhD8UmulWL1fIlyrLiFByVwnh9tp8T8Dy_X80W0fLpIZvdLiNJCO8ilepEKVrIKY-xYJLHrMSIci4RIYzyRJVSl5JqTqVMtSKqkIoIPDDNCsHjCbjc3W2d_Qjad3ld-c2DotE2-JwxRkhC8b8QTzmPURwP8GoHpbPeO13mratq4foco3zTTP7XzIAv9ldDUWv1S_dVxD_Ccoqg</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Switzer, W M</creator><creator>Owen, S M</creator><creator>Pieniazek, D A</creator><creator>Nerurkar, V R</creator><creator>Duenas-Barajas, E</creator><creator>Heneine, W</creator><creator>Lal, R B</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Molecular analysis of human T-cell lymphotropic virus type II from Wayuu Indians of Colombia demonstrates two subtypes of HTLV-IIb</title><author>Switzer, W M ; Owen, S M ; Pieniazek, D A ; Nerurkar, V R ; Duenas-Barajas, E ; Heneine, W ; Lal, R B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c228t-d9e4dd5bc6831a7c837f10588c0227584dfcefc5e85cc9ed2dbcd2a137fe7ba83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AIDS/HIV</topic><topic>Amino Acid Sequence</topic><topic>Antigens, Viral - immunology</topic><topic>Base Sequence</topic><topic>Cell Line, Transformed</topic><topic>Colombia - epidemiology</topic><topic>DNA, Viral - chemistry</topic><topic>DNA, Viral - genetics</topic><topic>Gene Products, tax - genetics</topic><topic>Genes, Viral</topic><topic>Genetic Heterogeneity</topic><topic>HTLV-II Infections - epidemiology</topic><topic>HTLV-II Infections - virology</topic><topic>Human T-lymphotropic virus 2 - classification</topic><topic>Human T-lymphotropic virus 2 - genetics</topic><topic>Human T-lymphotropic virus 2 - immunology</topic><topic>Human T-lymphotropic virus 2 - isolation & purification</topic><topic>Humans</topic><topic>Indians, South American</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Phylogeny</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Serotyping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Switzer, W M</creatorcontrib><creatorcontrib>Owen, S M</creatorcontrib><creatorcontrib>Pieniazek, D A</creatorcontrib><creatorcontrib>Nerurkar, V R</creatorcontrib><creatorcontrib>Duenas-Barajas, E</creatorcontrib><creatorcontrib>Heneine, W</creatorcontrib><creatorcontrib>Lal, R B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Switzer, W M</au><au>Owen, S M</au><au>Pieniazek, D A</au><au>Nerurkar, V R</au><au>Duenas-Barajas, E</au><au>Heneine, W</au><au>Lal, R B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of human T-cell lymphotropic virus type II from Wayuu Indians of Colombia demonstrates two subtypes of HTLV-IIb</atitle><jtitle>Virus genes</jtitle><addtitle>Virus Genes</addtitle><date>1995</date><risdate>1995</risdate><volume>10</volume><issue>2</issue><spage>153</spage><epage>162</epage><pages>153-162</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>Studies of the genetic heterogeneity of human T-cell lymphotropic virus type II (HTLV-II) have revealed the presence of two genetic subtypes, termed HTLV-IIa and HTLV-IIb. The HTLV-IIb subtype encodes an immunodominant epitope present at the C-terminus of the extended Tax protein and, by using an LTR-based, restriction fragment-length polymorphism (RFLP) assay, can be further classified into IIb60-IIb5, with HTLV-IIb1 (Central Amerindian-like) and HTLV-IIb5 (North Amerindian-like) being characteristic subtypes for Native American Indians. To determine the antigenic and genetic heterogeneity among HTLV-II-infected South Amerindians, we used a Tax synthetic peptide immunoassay on serum, and RFLP and phylogenetic analysis on LTR sequences amplified from genomic DNA from four Wayuu Indians of Colombia. The Wayuu specimens displayed seroreactivity to the immunodominant epitope located in the extended Tax region, as predicted, and demonstrated genetic heterogeneity by the presence of both the IIB1 (Wyu1, Zuc31) and IIb5 (Wyu2, Zuc42) subtypes sequences within separate phylogroups represented by the Guaymi Indian (IIb1) and North Amerindian (IIb5) sequences, respectively. Sequence analysis showed that major LTR regulatory motifs and the cis-acting repressive elements in the LTR RNA secondary structure were relatively conserved in both Wayuu subtypes, but the predicted secondary structure of the rex response stem loop in the Wyu2 (IIb5) LTR sequence was 45 nucleotides (nt) and 95 nt longer than that observed in the Wyu1 (IIb1) and G12.1 (IIb1) LTR sequences, respectively. These results extend our knowledge of the genetic heterogeneity of HTLV-II in South Amerindians.</abstract><cop>United States</cop><pmid>8560775</pmid><doi>10.1007/BF01702596</doi><tpages>10</tpages></addata></record> |
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| ispartof | Virus genes, 1995, Vol.10 (2), p.153-162 |
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| subjects | AIDS/HIV Amino Acid Sequence Antigens, Viral - immunology Base Sequence Cell Line, Transformed Colombia - epidemiology DNA, Viral - chemistry DNA, Viral - genetics Gene Products, tax - genetics Genes, Viral Genetic Heterogeneity HTLV-II Infections - epidemiology HTLV-II Infections - virology Human T-lymphotropic virus 2 - classification Human T-lymphotropic virus 2 - genetics Human T-lymphotropic virus 2 - immunology Human T-lymphotropic virus 2 - isolation & purification Humans Indians, South American Molecular Sequence Data Nucleic Acid Conformation Phylogeny Repetitive Sequences, Nucleic Acid Serotyping |
| title | Molecular analysis of human T-cell lymphotropic virus type II from Wayuu Indians of Colombia demonstrates two subtypes of HTLV-IIb |
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