Simultaneous detection of beta-galactosidase activity and surface antigen expression in viable haematopoietic cells

The quantitation of intracellular beta-galactosidase activity has been described for viable cells. By using the fluorogenic substrate fluorescein-di-beta-D-galactopyranoside (FDG) in conjunction with flow cytometry, the proportion of positive cells as well as the level of expression can be determine...

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Veröffentlicht in:	Cytometry (New York, N.Y.) N.Y.), 1994-11, Vol.17 (3), p.216-223
Hauptverfasser:	Berger, C N, Tan, S S, Sturm, K S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Surface - analysis Antigens, Surface - blood beta-Galactosidase - biosynthesis beta-Galactosidase - metabolism Biomarkers Crosses, Genetic Female Flow Cytometry Fluoresceins Galactosides Hematopoietic Stem Cells - enzymology Hematopoietic Stem Cells - immunology Liver - embryology Liver - enzymology Male Mice Mice, Transgenic Thymus Gland - embryology Thymus Gland - enzymology
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