Efficient purification and rigorous characterisation of a recombinant gp120 for HIV vaccine studies

A recombinant HIV-1 gp120 (rgp120) was expressed in a permanent Chinese Hamster Ovary (CHO) cell line (L761h) that constitutively secretes the product of clone p4 derived from the env gene of HIV-1 isolate GB8. The rgp120 was isolated from cell culture supernatants by a simple, rapid, non-denaturing...

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Veröffentlicht in:	Vaccine 1995, Vol.13 (11), p.991-999
Hauptverfasser:	Jones, David H., McBride, Brian W., Roff, Marilynn A., Farrar, Graham H.
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS Vaccines - chemistry AIDS Vaccines - immunology AIDS Vaccines - isolation & purification AIDS/HIV Amino Acid Sequence Animals Biological and medical sciences biological properties CD4 Antigens - chemistry CHO Cells Cricetinae Electrophoresis, Capillary Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology HIV Antibodies - biosynthesis HIV Antibodies - chemistry HIV Envelope Protein gp120 - chemistry HIV Envelope Protein gp120 - immunology HIV Envelope Protein gp120 - isolation & purification Human immunodeficiency virus immunological responses Mass Spectrometry Microbiology Molecular Sequence Data physical characterisation Protein Binding protein purification recombinant gp120 Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Synthetic - chemistry Vaccines, Synthetic - immunology Virology
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