Growth and viability of Bifidobacterium bifidum in Cheddar cheese

Bifidobacterium bifidum (ATCC 15696) was grown in MRS broth containig cysteine HCl at 37 degrees C, and the cells were harvested by centrifuging at 1300 X g for 15 min at 4 degrees C. Equal volumes of the cell slurry and a 2.5% solution of kappa-carrageenan were mixed and transferred by drops into a...

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Veröffentlicht in:Journal of dairy science 1994-10, Vol.77 (10), p.2854-2864
Hauptverfasser: Dinakar, P. (South Dakota State University, Brookings), Mistry, V.V
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container_end_page 2864
container_issue 10
container_start_page 2854
container_title Journal of dairy science
container_volume 77
creator Dinakar, P. (South Dakota State University, Brookings)
Mistry, V.V
description Bifidobacterium bifidum (ATCC 15696) was grown in MRS broth containig cysteine HCl at 37 degrees C, and the cells were harvested by centrifuging at 1300 X g for 15 min at 4 degrees C. Equal volumes of the cell slurry and a 2.5% solution of kappa-carrageenan were mixed and transferred by drops into a solution of.3 M KCl at 20 degrees C under an atmosphere of nitrogen. The gelled beads were separated, frozen, and lyophilized immediately. This preparation and a commercial powder preparation were added to Cheddar cheese curd at milling as two treatments. Treatments did not affect cheese composition. Soluble protein increased during ripening at 7 degrees C but without differences between treatments; SDS-PAGE patterns of proteolysis were also similar. Lactic acid content of cheeses increased during ripening, but differences between treatments were minor. Acetic acid and ethanol, common metabolites of bifidobacteria, were not detected during ripening. Bifidobacteria remained viable and increased in numbers in cheese during this 24-wk study but did not affect the flavor, flavor intensity, texture, or appearance of the cheese compared with that of the control
doi_str_mv 10.3168/jds.S0022-0302(94)77225-8
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Lactic acid content of cheeses increased during ripening, but differences between treatments were minor. Acetic acid and ethanol, common metabolites of bifidobacteria, were not detected during ripening. Bifidobacteria remained viable and increased in numbers in cheese during this 24-wk study but did not affect the flavor, flavor intensity, texture, or appearance of the cheese compared with that of the control</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.S0022-0302(94)77225-8</identifier><identifier>PMID: 7836576</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>Savoy, IL: Am Dairy Sci Assoc</publisher><subject>ACIDE LACTIQUE ; ACIDO LACTICO ; ANALISIS ORGANOLEPTICO ; ANALYSE ORGANOLEPTIQUE ; BACTERIA ; BIFIDOBACTERIUM ; Bifidobacterium - growth &amp; development ; Bifidobacterium - physiology ; Bifidobacterium bifidum ; Biological and medical sciences ; Cheese - microbiology ; Chromatography, High Pressure Liquid ; CONTEO DE CELULAS ; Dietary Proteins - metabolism ; Food industries ; FROMAGE ; Fundamental and applied biological sciences. 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(South Dakota State University, Brookings)</au><au>Mistry, V.V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth and viability of Bifidobacterium bifidum in Cheddar cheese</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>1994-10-01</date><risdate>1994</risdate><volume>77</volume><issue>10</issue><spage>2854</spage><epage>2864</epage><pages>2854-2864</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><coden>JDSCAE</coden><abstract>Bifidobacterium bifidum (ATCC 15696) was grown in MRS broth containig cysteine HCl at 37 degrees C, and the cells were harvested by centrifuging at 1300 X g for 15 min at 4 degrees C. Equal volumes of the cell slurry and a 2.5% solution of kappa-carrageenan were mixed and transferred by drops into a solution of.3 M KCl at 20 degrees C under an atmosphere of nitrogen. The gelled beads were separated, frozen, and lyophilized immediately. This preparation and a commercial powder preparation were added to Cheddar cheese curd at milling as two treatments. Treatments did not affect cheese composition. Soluble protein increased during ripening at 7 degrees C but without differences between treatments; SDS-PAGE patterns of proteolysis were also similar. Lactic acid content of cheeses increased during ripening, but differences between treatments were minor. Acetic acid and ethanol, common metabolites of bifidobacteria, were not detected during ripening. Bifidobacteria remained viable and increased in numbers in cheese during this 24-wk study but did not affect the flavor, flavor intensity, texture, or appearance of the cheese compared with that of the control</abstract><cop>Savoy, IL</cop><pub>Am Dairy Sci Assoc</pub><pmid>7836576</pmid><doi>10.3168/jds.S0022-0302(94)77225-8</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
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subjects ACIDE LACTIQUE
ACIDO LACTICO
ANALISIS ORGANOLEPTICO
ANALYSE ORGANOLEPTIQUE
BACTERIA
BIFIDOBACTERIUM
Bifidobacterium - growth & development
Bifidobacterium - physiology
Bifidobacterium bifidum
Biological and medical sciences
Cheese - microbiology
Chromatography, High Pressure Liquid
CONTEO DE CELULAS
Dietary Proteins - metabolism
Food industries
FROMAGE
Fundamental and applied biological sciences. Psychology
MADURAMIENTO
Microscopy, Electron, Scanning
Milk and cheese industries. Ice creams
MURISSAGE
NUMERATION CELLULAIRE
QUESO
Taste
Time Factors
VIABILIDAD
VIABILITE
title Growth and viability of Bifidobacterium bifidum in Cheddar cheese
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