Rapid detection of bovine herpesvirus 1 (BHV 1) using the polymerase chain reaction
A polymerase chain reaction (PCR) assay based on primers from the viral gl glycoprotein gene detected 3 fg pure BHV-1 DNA, 0.1 TCID 50 or a single infected cell. No amplification was observed with DNA from BHV-2, BHV-3, BHV-4, OHV-1 or OHV-2. However, a fragment of the correct size was amplified usi...
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| Veröffentlicht in: | Veterinary microbiology 1994-09, Vol.42 (1), p.53-64 |
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| creator | Vilcek, S. Nettleton, P.F. Herring, J.A. Herring, A.J. |
| description | A polymerase chain reaction (PCR) assay based on primers from the viral gl glycoprotein gene detected 3 fg pure BHV-1 DNA, 0.1 TCID
50 or a single infected cell. No amplification was observed with DNA from BHV-2, BHV-3, BHV-4, OHV-1 or OHV-2. However, a fragment of the correct size was amplified using DNA from herpesvirus isolated from reindeer, red deer and goats. The PCR assay was able to detect virus in nasal swabs up to 14 days after experimental infection of cattle and there was a good correlation when PCR was compared with virus isolation for the detection of BHV-1 in clinical field samples. Detection of BHV-1 in fetal bovine serum and semen samples was also successful. |
| doi_str_mv | 10.1016/0378-1135(94)90077-9 |
| format | Article |
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50 or a single infected cell. No amplification was observed with DNA from BHV-2, BHV-3, BHV-4, OHV-1 or OHV-2. However, a fragment of the correct size was amplified using DNA from herpesvirus isolated from reindeer, red deer and goats. The PCR assay was able to detect virus in nasal swabs up to 14 days after experimental infection of cattle and there was a good correlation when PCR was compared with virus isolation for the detection of BHV-1 in clinical field samples. Detection of BHV-1 in fetal bovine serum and semen samples was also successful.</description><subject>ADN</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>BOVIN</subject><subject>BOVINE HERPESVIRUS</subject><subject>Bovine herpesvirus 1</subject><subject>CATTLE</subject><subject>Cattle Diseases - diagnosis</subject><subject>DIAGNOSIS</subject><subject>DIAGNOSTIC</subject><subject>DIAGNOSTICO</subject><subject>DNA</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>Fetal Blood - virology</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>GANADO BOVINO</topic><topic>GENE</topic><topic>GENES</topic><topic>Genetics</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>GLYCOPROTEINS</topic><topic>Herpesviridae Infections - diagnosis</topic><topic>Herpesviridae Infections - veterinary</topic><topic>Herpesvirus 1, Bovine - isolation & purification</topic><topic>HERPESVIRUS BOVIN</topic><topic>HERPESVIRUS BOVINO</topic><topic>METHODE</topic><topic>METHODS</topic><topic>METODOS</topic><topic>Microbiology</topic><topic>NARIZ</topic><topic>NEZ</topic><topic>NOSE</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>SEMEN</topic><topic>Semen - virology</topic><topic>Sensitivity and Specificity</topic><topic>SPERME</topic><topic>Time Factors</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vilcek, S.</creatorcontrib><creatorcontrib>Nettleton, P.F.</creatorcontrib><creatorcontrib>Herring, J.A.</creatorcontrib><creatorcontrib>Herring, A.J.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vilcek, S.</au><au>Nettleton, P.F.</au><au>Herring, J.A.</au><au>Herring, A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of bovine herpesvirus 1 (BHV 1) using the polymerase chain reaction</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>42</volume><issue>1</issue><spage>53</spage><epage>64</epage><pages>53-64</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>A polymerase chain reaction (PCR) assay based on primers from the viral gl glycoprotein gene detected 3 fg pure BHV-1 DNA, 0.1 TCID
50 or a single infected cell. No amplification was observed with DNA from BHV-2, BHV-3, BHV-4, OHV-1 or OHV-2. However, a fragment of the correct size was amplified using DNA from herpesvirus isolated from reindeer, red deer and goats. The PCR assay was able to detect virus in nasal swabs up to 14 days after experimental infection of cattle and there was a good correlation when PCR was compared with virus isolation for the detection of BHV-1 in clinical field samples. Detection of BHV-1 in fetal bovine serum and semen samples was also successful.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>7839585</pmid><doi>10.1016/0378-1135(94)90077-9</doi><tpages>12</tpages></addata></record> |
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| ispartof | Veterinary microbiology, 1994-09, Vol.42 (1), p.53-64 |
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| subjects | ADN Animals Biological and medical sciences BOVIN BOVINE HERPESVIRUS Bovine herpesvirus 1 CATTLE Cattle Diseases - diagnosis DIAGNOSIS DIAGNOSTIC DIAGNOSTICO DNA DNA, Viral - analysis Female Fetal Blood - virology Fundamental and applied biological sciences. Psychology GANADO BOVINO GENE GENES Genetics GLICOPROTEINAS GLYCOPROTEINE GLYCOPROTEINS Herpesviridae Infections - diagnosis Herpesviridae Infections - veterinary Herpesvirus 1, Bovine - isolation & purification HERPESVIRUS BOVIN HERPESVIRUS BOVINO METHODE METHODS METODOS Microbiology NARIZ NEZ NOSE Polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary SEMEN Semen - virology Sensitivity and Specificity SPERME Time Factors Virology |
| title | Rapid detection of bovine herpesvirus 1 (BHV 1) using the polymerase chain reaction |
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