Determination of imipenem and cilastatin sodium in Primaxin® by first order derivative ultraviolet spectrophotometry
A UV-spectrophotometric assay to measure the concentrations of the active drug components (imipenem and cilastatin) or Primaxin® for routine release testing is described. The assay is based on the use of first order derivative spectrophotometry. The trough amplitudes in the first derivative spectrop...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 1994-10, Vol.12 (10), p.1243-1248 |
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| creator | Forsyth, R.J. Ip, D.P. |
| description | A UV-spectrophotometric assay to measure the concentrations of the active drug components (imipenem and cilastatin) or Primaxin® for routine release testing is described. The assay is based on the use of first order derivative spectrophotometry. The trough amplitudes in the first derivative spectrophotometric spectra at 243 and 318 nm were selected to determine cilastatin and imipenem, respectively. A linear relationship (
R > 0.99) between the trough amplitudes and concentrations was demonstrated over the range 14–42 μg ml
−1 for both drug components. Commercial IV formulations and laboratory prepared mixtures containing both drugs in different proportions were assayed using the developed method with good recoveries (ave. 100.6%). The method is rapid, precise, accurate and was shown to be equivalent to the more time consuming LC method; which is currently used for routine release testing. The specificity and stability indicating properties of the method will also be addressed. |
| doi_str_mv | 10.1016/0731-7085(94)00067-0 |
| format | Article |
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R > 0.99) between the trough amplitudes and concentrations was demonstrated over the range 14–42 μg ml
−1 for both drug components. Commercial IV formulations and laboratory prepared mixtures containing both drugs in different proportions were assayed using the developed method with good recoveries (ave. 100.6%). The method is rapid, precise, accurate and was shown to be equivalent to the more time consuming LC method; which is currently used for routine release testing. The specificity and stability indicating properties of the method will also be addressed.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/0731-7085(94)00067-0</identifier><identifier>PMID: 7841218</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Biological and medical sciences ; Buffers ; Chromatography, Liquid ; Cilastatin - analysis ; cilastatin sodium ; Cilastatin, Imipenem Drug Combination ; derivative UV spectrophotometry ; Drug Combinations ; Drug Therapy, Combination - analysis ; General pharmacology ; imipenem ; Imipenem - analysis ; Medical sciences ; Morpholines ; Pharmacology. Drug treatments ; Primaxin ; Spectrophotometry, Ultraviolet</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 1994-10, Vol.12 (10), p.1243-1248</ispartof><rights>1994</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-e89cf26fbdffdf454faf68d8db4d1ca6ac43f3d1364bacc5c7f602a4f700e5e13</citedby><cites>FETCH-LOGICAL-c452t-e89cf26fbdffdf454faf68d8db4d1ca6ac43f3d1364bacc5c7f602a4f700e5e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0731-7085(94)00067-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3304438$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7841218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Forsyth, R.J.</creatorcontrib><creatorcontrib>Ip, D.P.</creatorcontrib><title>Determination of imipenem and cilastatin sodium in Primaxin® by first order derivative ultraviolet spectrophotometry</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A UV-spectrophotometric assay to measure the concentrations of the active drug components (imipenem and cilastatin) or Primaxin® for routine release testing is described. The assay is based on the use of first order derivative spectrophotometry. The trough amplitudes in the first derivative spectrophotometric spectra at 243 and 318 nm were selected to determine cilastatin and imipenem, respectively. A linear relationship (
R > 0.99) between the trough amplitudes and concentrations was demonstrated over the range 14–42 μg ml
−1 for both drug components. Commercial IV formulations and laboratory prepared mixtures containing both drugs in different proportions were assayed using the developed method with good recoveries (ave. 100.6%). The method is rapid, precise, accurate and was shown to be equivalent to the more time consuming LC method; which is currently used for routine release testing. The specificity and stability indicating properties of the method will also be addressed.</description><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>Chromatography, Liquid</subject><subject>Cilastatin - analysis</subject><subject>cilastatin sodium</subject><subject>Cilastatin, Imipenem Drug Combination</subject><subject>derivative UV spectrophotometry</subject><subject>Drug Combinations</subject><subject>Drug Therapy, Combination - analysis</subject><subject>General pharmacology</subject><subject>imipenem</subject><subject>Imipenem - analysis</subject><subject>Medical sciences</subject><subject>Morpholines</subject><subject>Pharmacology. Drug treatments</subject><subject>Primaxin</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM-KFDEQh4Mo6-zqGyjkILIeek066aTnsiDrnxUW1oOCt5BOKhjpTtokPTgv5UP4ZKadYY57KFLw-6qofAi9oOSKEireEsloI0nfXW75G0KIkA15hDa0l6xpBf_-GG1OyFN0nvPPCnV0y8_Qmew5bWm_Qct7KJAmH3TxMeDosJ_8DAEmrIPFxo86l5oFnKP1y4Rr9yX5Sf_24e8fPOyx8ykXHJOFhGv5XaV3gJexJL3zcYSC8wympDj_iCVOUNL-GXri9Jjh-fG9QN8-fvh6c9vc3X_6fPPurjG8a0sD_da4VrjBOmcd77jTTvS2twO31GihDWeOWcoEH7QxnZFOkFZzJwmBDii7QK8Pe-cUfy2Qi5p8NjCOOkBcspJSUiG7voL8AJoUc07g1Lx-Mu0VJWq1rVaValWptlz9t61IHXt53L8ME9jT0FFvzV8dc52NHl3Swfh8whgjnLMVuz5gUF3sPCSVjYdgwPpUzSkb_cN3_AMnCqCI</recordid><startdate>19941001</startdate><enddate>19941001</enddate><creator>Forsyth, R.J.</creator><creator>Ip, D.P.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941001</creationdate><title>Determination of imipenem and cilastatin sodium in Primaxin® by first order derivative ultraviolet spectrophotometry</title><author>Forsyth, R.J. ; Ip, D.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-e89cf26fbdffdf454faf68d8db4d1ca6ac43f3d1364bacc5c7f602a4f700e5e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Buffers</topic><topic>Chromatography, Liquid</topic><topic>Cilastatin - analysis</topic><topic>cilastatin sodium</topic><topic>Cilastatin, Imipenem Drug Combination</topic><topic>derivative UV spectrophotometry</topic><topic>Drug Combinations</topic><topic>Drug Therapy, Combination - analysis</topic><topic>General pharmacology</topic><topic>imipenem</topic><topic>Imipenem - analysis</topic><topic>Medical sciences</topic><topic>Morpholines</topic><topic>Pharmacology. Drug treatments</topic><topic>Primaxin</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Forsyth, R.J.</creatorcontrib><creatorcontrib>Ip, D.P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Forsyth, R.J.</au><au>Ip, D.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of imipenem and cilastatin sodium in Primaxin® by first order derivative ultraviolet spectrophotometry</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>1994-10-01</date><risdate>1994</risdate><volume>12</volume><issue>10</issue><spage>1243</spage><epage>1248</epage><pages>1243-1248</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A UV-spectrophotometric assay to measure the concentrations of the active drug components (imipenem and cilastatin) or Primaxin® for routine release testing is described. The assay is based on the use of first order derivative spectrophotometry. The trough amplitudes in the first derivative spectrophotometric spectra at 243 and 318 nm were selected to determine cilastatin and imipenem, respectively. A linear relationship (
R > 0.99) between the trough amplitudes and concentrations was demonstrated over the range 14–42 μg ml
−1 for both drug components. Commercial IV formulations and laboratory prepared mixtures containing both drugs in different proportions were assayed using the developed method with good recoveries (ave. 100.6%). The method is rapid, precise, accurate and was shown to be equivalent to the more time consuming LC method; which is currently used for routine release testing. The specificity and stability indicating properties of the method will also be addressed.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>7841218</pmid><doi>10.1016/0731-7085(94)00067-0</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical and biomedical analysis, 1994-10, Vol.12 (10), p.1243-1248 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis Biological and medical sciences Buffers Chromatography, Liquid Cilastatin - analysis cilastatin sodium Cilastatin, Imipenem Drug Combination derivative UV spectrophotometry Drug Combinations Drug Therapy, Combination - analysis General pharmacology imipenem Imipenem - analysis Medical sciences Morpholines Pharmacology. Drug treatments Primaxin Spectrophotometry, Ultraviolet |
| title | Determination of imipenem and cilastatin sodium in Primaxin® by first order derivative ultraviolet spectrophotometry |
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