Preliminary crystallographic analysis of an enzyme involved in erythromycin biosynthesis: Cytochrome P450eryF

Cytochrome P450eryF was overexpressed in Escherichia coli and purified in high yield. Crystals of the protein in the presence of the substrate, 6‐deoxyerythronolide B, have been obtained by the hanging drop vapor diffusion method, using polyethylene glycol 4000 as a precipitant. The crystals belong...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proteins, structure, function, and bioinformatics structure, function, and bioinformatics, 1994-10, Vol.20 (2), p.197-201
Hauptverfasser:	Cupp-Vickery, Jill R., Li, Huiying, Poulos, Thomas L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Proteins crystallization Crystallography, X-Ray Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics cytochrome P450 erythromycin Erythromycin - analogs & derivatives Erythromycin - biosynthesis Erythromycin - metabolism Escherichia coli Escherichia coli - genetics Mixed Function Oxygenases - chemistry Mixed Function Oxygenases - genetics Molecular Sequence Data P450eryF Recombinant Proteins - chemistry Saccharopolyspora - enzymology Saccharopolyspora - genetics Saccharopolyspora erythraea Sequence Homology, Amino Acid
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!