Sequential Analysis of Gene Expression after an Osteogenic Stimulus - c-fos Expression Is Induced in Osteocytes
We have recently developed an experimental model whereby mechanical stimulation induces osteogenesis in the caudal vertebrae of rats. We used this model to assess expression of genes induced by mechanical loading. Bulk preparations of mRNA extracted after loading did not-show >2-fold increases in...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1995-12, Vol.217 (1), p.264-270 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Inaoka, T. Lean, J.M. Bessho, T. Chow, J.W.M. Mackay, A. Kokubo, T. Chambers, T.J. |
| description | We have recently developed an experimental model whereby mechanical stimulation induces osteogenesis in the caudal vertebrae of rats. We used this model to assess expression of genes induced by mechanical loading. Bulk preparations of mRNA extracted after loading did not-show >2-fold increases in expression of mRNA for matrix proteins or growth factors in Northern blotting analysis. c-jun was undetectable. However, c-fos showed a 4-fold increase in expression within 60 mins of loading, before returning to control levels by 4 hrs. This increase was associated with intense signals in in situ hybridization, not seen in any nonloaded vertebrae, for c-fos over cortical osteocytes: thus osteocytes respond to mechanical loading with c-fos expression so strongly as to be visible even in the bulk RNA preparations. The results represent persuasive evidence for a role for osteocytes, and for c-fos, in the osteogenic response of bone to mechanical stimulation. |
| doi_str_mv | 10.1006/bbrc.1995.2773 |
| format | Article |
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We used this model to assess expression of genes induced by mechanical loading. Bulk preparations of mRNA extracted after loading did not-show >2-fold increases in expression of mRNA for matrix proteins or growth factors in Northern blotting analysis. c-jun was undetectable. However, c-fos showed a 4-fold increase in expression within 60 mins of loading, before returning to control levels by 4 hrs. This increase was associated with intense signals in in situ hybridization, not seen in any nonloaded vertebrae, for c-fos over cortical osteocytes: thus osteocytes respond to mechanical loading with c-fos expression so strongly as to be visible even in the bulk RNA preparations. The results represent persuasive evidence for a role for osteocytes, and for c-fos, in the osteogenic response of bone to mechanical stimulation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1995.2773</identifier><identifier>PMID: 8526921</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bone Matrix - metabolism ; Female ; Gene Expression ; Genes, fos ; Growth Substances - genetics ; In Situ Hybridization ; Models, Biological ; Osteocytes - metabolism ; Osteogenesis - genetics ; Proteins - genetics ; Rats ; Rats, Wistar ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Space life sciences ; Stress, Mechanical</subject><ispartof>Biochemical and biophysical research communications, 1995-12, Vol.217 (1), p.264-270</ispartof><rights>1995 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-b09ef86af391343f70cf334489ffe6aceb9042126312541f9d7d97bd4b7d54323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1995.2773$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8526921$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inaoka, T.</creatorcontrib><creatorcontrib>Lean, J.M.</creatorcontrib><creatorcontrib>Bessho, T.</creatorcontrib><creatorcontrib>Chow, J.W.M.</creatorcontrib><creatorcontrib>Mackay, A.</creatorcontrib><creatorcontrib>Kokubo, T.</creatorcontrib><creatorcontrib>Chambers, T.J.</creatorcontrib><title>Sequential Analysis of Gene Expression after an Osteogenic Stimulus - c-fos Expression Is Induced in Osteocytes</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We have recently developed an experimental model whereby mechanical stimulation induces osteogenesis in the caudal vertebrae of rats. We used this model to assess expression of genes induced by mechanical loading. Bulk preparations of mRNA extracted after loading did not-show >2-fold increases in expression of mRNA for matrix proteins or growth factors in Northern blotting analysis. c-jun was undetectable. However, c-fos showed a 4-fold increase in expression within 60 mins of loading, before returning to control levels by 4 hrs. This increase was associated with intense signals in in situ hybridization, not seen in any nonloaded vertebrae, for c-fos over cortical osteocytes: thus osteocytes respond to mechanical loading with c-fos expression so strongly as to be visible even in the bulk RNA preparations. The results represent persuasive evidence for a role for osteocytes, and for c-fos, in the osteogenic response of bone to mechanical stimulation.</description><subject>Animals</subject><subject>Bone Matrix - metabolism</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Genes, fos</subject><subject>Growth Substances - genetics</subject><subject>In Situ Hybridization</subject><subject>Models, Biological</subject><subject>Osteocytes - metabolism</subject><subject>Osteogenesis - genetics</subject><subject>Proteins - genetics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Space life sciences</subject><subject>Stress, Mechanical</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1rGzEURUVpcB232-4KWnU3rr5mZC1NSF1DwAs30J3QaJ6Cwlhy9WZK_e8zxiZkk9Vb3HMvvEPIV86WnLHmR9sWv-TG1EuhtfxA5pwZVgnO1EcyZxNRCcP_fCK3iM-Mca4aMyOzVS0aI_ic5D38HSEN0fV0nVx_wog0B7qBBPT-_7EAYsyJujBAoS7RHQ6QnyBFT_dDPIz9iLSivgoZ3_JbpNvUjR46Gq8lfxoAP5Ob4HqEL9e7II8_73_f_aoedpvt3fqh8orVQ9UyA2HVuCANl0oGzXyQUqmVCQEa56E1TAkuGslFrXgwne6MbjvV6q5WUsgF-X7ZPZY8PYiDPUT00PcuQR7Raq25YNxM4PIC-pIRCwR7LPHgyslyZs-G7dmwPRu2Z8NT4dt1eWwP0L3iV6VTvrrkML33L0Kx6COkyUQs4Afb5fje9AvuAIrt</recordid><startdate>19951205</startdate><enddate>19951205</enddate><creator>Inaoka, T.</creator><creator>Lean, J.M.</creator><creator>Bessho, T.</creator><creator>Chow, J.W.M.</creator><creator>Mackay, A.</creator><creator>Kokubo, T.</creator><creator>Chambers, T.J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951205</creationdate><title>Sequential Analysis of Gene Expression after an Osteogenic Stimulus - c-fos Expression Is Induced in Osteocytes</title><author>Inaoka, T. ; Lean, J.M. ; Bessho, T. ; Chow, J.W.M. ; Mackay, A. ; Kokubo, T. ; Chambers, T.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-b09ef86af391343f70cf334489ffe6aceb9042126312541f9d7d97bd4b7d54323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Bone Matrix - metabolism</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Genes, fos</topic><topic>Growth Substances - genetics</topic><topic>In Situ Hybridization</topic><topic>Models, Biological</topic><topic>Osteocytes - metabolism</topic><topic>Osteogenesis - genetics</topic><topic>Proteins - genetics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Space life sciences</topic><topic>Stress, Mechanical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inaoka, T.</creatorcontrib><creatorcontrib>Lean, J.M.</creatorcontrib><creatorcontrib>Bessho, T.</creatorcontrib><creatorcontrib>Chow, J.W.M.</creatorcontrib><creatorcontrib>Mackay, A.</creatorcontrib><creatorcontrib>Kokubo, T.</creatorcontrib><creatorcontrib>Chambers, T.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inaoka, T.</au><au>Lean, J.M.</au><au>Bessho, T.</au><au>Chow, J.W.M.</au><au>Mackay, A.</au><au>Kokubo, T.</au><au>Chambers, T.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequential Analysis of Gene Expression after an Osteogenic Stimulus - c-fos Expression Is Induced in Osteocytes</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1995-12-05</date><risdate>1995</risdate><volume>217</volume><issue>1</issue><spage>264</spage><epage>270</epage><pages>264-270</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We have recently developed an experimental model whereby mechanical stimulation induces osteogenesis in the caudal vertebrae of rats. We used this model to assess expression of genes induced by mechanical loading. Bulk preparations of mRNA extracted after loading did not-show >2-fold increases in expression of mRNA for matrix proteins or growth factors in Northern blotting analysis. c-jun was undetectable. However, c-fos showed a 4-fold increase in expression within 60 mins of loading, before returning to control levels by 4 hrs. This increase was associated with intense signals in in situ hybridization, not seen in any nonloaded vertebrae, for c-fos over cortical osteocytes: thus osteocytes respond to mechanical loading with c-fos expression so strongly as to be visible even in the bulk RNA preparations. The results represent persuasive evidence for a role for osteocytes, and for c-fos, in the osteogenic response of bone to mechanical stimulation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8526921</pmid><doi>10.1006/bbrc.1995.2773</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1995-12, Vol.217 (1), p.264-270 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Bone Matrix - metabolism Female Gene Expression Genes, fos Growth Substances - genetics In Situ Hybridization Models, Biological Osteocytes - metabolism Osteogenesis - genetics Proteins - genetics Rats Rats, Wistar RNA, Messenger - genetics RNA, Messenger - metabolism Space life sciences Stress, Mechanical |
| title | Sequential Analysis of Gene Expression after an Osteogenic Stimulus - c-fos Expression Is Induced in Osteocytes |
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