Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector

Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector

An Escherichia coli-Bifidobacterium longum shuttle vector, designated pRM2, was constructed by cloning a B. longum plasmid and an enterococcal spectinomycin resistance gene into a commercial E. coli vector. The plasmid was successfully introduced into B. longum cells by electroporation and into E. c...

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Veröffentlicht in:Plasmid 1994-09, Vol.32 (2), p.208-211
Hauptverfasser: Missich, R, Sgorbati, B, LeBlanc, D.J
Format: Artikel
Sprache:eng
Schlagworte:
Bifidobacterium - drug effects
Bifidobacterium - genetics
Bifidobacterium longum
DNA, Bacterial - genetics
DNA, Recombinant
Drug Resistance, Microbial - genetics
Electroporation
Escherichia coli
Escherichia coli - genetics
genetic transformation
Genetic Vectors
plasmid vectors
Species Specificity
Spectinomycin - pharmacology
Transformation, Bacterial
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Beschreibung
Zusammenfassung:An Escherichia coli-Bifidobacterium longum shuttle vector, designated pRM2, was constructed by cloning a B. longum plasmid and an enterococcal spectinomycin resistance gene into a commercial E. coli vector. The plasmid was successfully introduced into B. longum cells by electroporation and into E. coli cells by both electroporation and chemical transformation.
ISSN:0147-619X
1095-9890
DOI:10.1006/plas.1994.1056