Enzymatic and immunological activity of 4000 years aged bone alkaline phosphatase
Structurally intact and functionally active human bone alkaline phosphatase was isolated from clavicle fragments of IDU, an Egyptian mummy of the Old Kingdom (2150 ± 50 BC). Both anion exchange and affinity chromatographies were employed to optimise the preparation of the ancient enzyme resulting in...
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| Veröffentlicht in: | FEBS letters 1995-11, Vol.375 (3), p.280-282 |
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| creator | Weser, Ulrich Etspüler, Hedwig Kaup, Yoka |
| description | Structurally intact and functionally active human bone alkaline phosphatase was isolated from clavicle fragments of IDU, an Egyptian mummy of the Old Kingdom (2150 ± 50 BC). Both anion exchange and affinity chromatographies were employed to optimise the preparation of the ancient enzyme resulting in a specific activity of 180 ± 30 mU/mg. The intactness of the bone enzyme fractions of the wheat-germ lectin affinity chromatography was successfully demonstrated in an ELISA using the monoclonal antibody BAP A. Fortunately, the mummified bone was not contaminated by fungi or bacteria. |
| doi_str_mv | 10.1016/0014-5793(95)01227-6 |
| format | Article |
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Fortunately, the mummified bone was not contaminated by fungi or bacteria.</description><subject>Alkaline Phosphatase - immunology</subject><subject>Alkaline Phosphatase - isolation & purification</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Ancient enzyme</subject><subject>Antibodies, Monoclonal</subject><subject>Binding Sites</subject><subject>Bone alkaline phosphatase</subject><subject>Bone and Bones - enzymology</subject><subject>Chromatography, Affinity</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Ion Exchange</subject><subject>Clavicle</subject><subject>Egypt</subject><subject>Egyptian mummy</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Humans</subject><subject>Isoenzymes - immunology</subject><subject>Isoenzymes - isolation & purification</subject><subject>Isoenzymes - metabolism</subject><subject>Kinetics</subject><subject>Monoclonal antibody</subject><subject>Mummies</subject><subject>Zinc - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtu1DAUhi0EKkPhDUDyCsEirY-v8QapVDOAVKlCgrXlsU9aQxIPcaYofXqSzqhLYGX7_BcffYS8BnYGDPQ5YyArZax4Z9V7BpybSj8hK6iNqITU9VOyerQ8Jy9K-cHmdw32hJwYaWsF9Yp8Xff3U-fHFKjvI01dt-9zm29S8C31YUx3aZxobqicw3RCPxTqbzDSbe6R-vanb9N82d3msrv1oy_4kjxrfFvw1fE8Jd8362-Xn6ur609fLi-uqiCt0ZUHpaWolRXaQ2BiXsgzMAa8lcFqZWPDa1bzrZCoQGDkHKLmDVj02tooTsnbQ-9uyL_2WEbXpRKwbX2PeV-cMYYZCeKfRjBMCWH4bJQHYxhyKQM2bjekzg-TA-YW5G7h6Raezir3gNzpOfbm2L_fdhgfQ0fGs7456L9Ti9N_dbrN-iNfhGVu1cN0-ejDoQhnrHcJB1dCwj5gTAOG0cWc_r7pH9IWoRQ</recordid><startdate>19951120</startdate><enddate>19951120</enddate><creator>Weser, Ulrich</creator><creator>Etspüler, Hedwig</creator><creator>Kaup, Yoka</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>19951120</creationdate><title>Enzymatic and immunological activity of 4000 years aged bone alkaline phosphatase</title><author>Weser, Ulrich ; Etspüler, Hedwig ; Kaup, Yoka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4976-a1564385936a1c03498a01771a94c9659df28082b34e513ed221d62f19ea699d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Alkaline Phosphatase - immunology</topic><topic>Alkaline Phosphatase - isolation & purification</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Ancient enzyme</topic><topic>Antibodies, Monoclonal</topic><topic>Binding Sites</topic><topic>Bone alkaline phosphatase</topic><topic>Bone and Bones - enzymology</topic><topic>Chromatography, Affinity</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Ion Exchange</topic><topic>Clavicle</topic><topic>Egypt</topic><topic>Egyptian mummy</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Humans</topic><topic>Isoenzymes - immunology</topic><topic>Isoenzymes - isolation & purification</topic><topic>Isoenzymes - metabolism</topic><topic>Kinetics</topic><topic>Monoclonal antibody</topic><topic>Mummies</topic><topic>Zinc - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weser, Ulrich</creatorcontrib><creatorcontrib>Etspüler, Hedwig</creatorcontrib><creatorcontrib>Kaup, Yoka</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weser, Ulrich</au><au>Etspüler, Hedwig</au><au>Kaup, Yoka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic and immunological activity of 4000 years aged bone alkaline phosphatase</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1995-11-20</date><risdate>1995</risdate><volume>375</volume><issue>3</issue><spage>280</spage><epage>282</epage><pages>280-282</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Structurally intact and functionally active human bone alkaline phosphatase was isolated from clavicle fragments of IDU, an Egyptian mummy of the Old Kingdom (2150 ± 50 BC). Both anion exchange and affinity chromatographies were employed to optimise the preparation of the ancient enzyme resulting in a specific activity of 180 ± 30 mU/mg. The intactness of the bone enzyme fractions of the wheat-germ lectin affinity chromatography was successfully demonstrated in an ELISA using the monoclonal antibody BAP A. Fortunately, the mummified bone was not contaminated by fungi or bacteria.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>7498518</pmid><doi>10.1016/0014-5793(95)01227-6</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | FEBS letters, 1995-11, Vol.375 (3), p.280-282 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Alkaline Phosphatase - immunology Alkaline Phosphatase - isolation & purification Alkaline Phosphatase - metabolism Ancient enzyme Antibodies, Monoclonal Binding Sites Bone alkaline phosphatase Bone and Bones - enzymology Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Clavicle Egypt Egyptian mummy ELISA Enzyme-Linked Immunosorbent Assay Humans Isoenzymes - immunology Isoenzymes - isolation & purification Isoenzymes - metabolism Kinetics Monoclonal antibody Mummies Zinc - metabolism |
| title | Enzymatic and immunological activity of 4000 years aged bone alkaline phosphatase |
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