Characterization of the Phosphatidylinositol-Glycan Membrane Anchor of Human Placental Alkaline Phosphatase
Placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] is a member of a diverse group of membrane proteins whose attachment to the lipid bilayer is mediated by a phosphatidylinositol-glycan. To investigate structural aspects of the glycolipid ancho...
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| Veröffentlicht in: | Proc. Natl. Acad. Sci. U.S.A.; (United States) 1987-09, Vol.84 (17), p.6055-6059 |
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| creator | Howard, Andrew D. Berger, Joel Gerber, Louise Familletti, Philip Udenfriend, Sidney |
| description | Placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] is a member of a diverse group of membrane proteins whose attachment to the lipid bilayer is mediated by a phosphatidylinositol-glycan. To investigate structural aspects of the glycolipid anchor, cultured WISH cells were used because we found that they produce the enzyme in abundant quantities. When cell suspensions were incubated with purified phosphatidylinositol-specific phospholipase C, most of the placental alkaline phosphatase was released from membranes in a hydrophilic form. On incubation of the cells with [14C]ethanolamine, [14C]myristic acid, or myo[3H]inositol, each was incorporated into the phosphatase near the carboxyl terminus, showing that these components, which are found in other phosphatidylinositol membrane-linked proteins, are also present in placental alkaline phosphatase. |
| doi_str_mv | 10.1073/pnas.84.17.6055 |
| format | Article |
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To investigate structural aspects of the glycolipid anchor, cultured WISH cells were used because we found that they produce the enzyme in abundant quantities. When cell suspensions were incubated with purified phosphatidylinositol-specific phospholipase C, most of the placental alkaline phosphatase was released from membranes in a hydrophilic form. On incubation of the cells with [14C]ethanolamine, [14C]myristic acid, or myo[3H]inositol, each was incorporated into the phosphatase near the carboxyl terminus, showing that these components, which are found in other phosphatidylinositol membrane-linked proteins, are also present in placental alkaline phosphatase.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.84.17.6055</identifier><identifier>PMID: 2819864</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>550201 - Biochemistry- Tracer Techniques ; ALKALINE PHOSPHATASE ; Alkaline Phosphatase - metabolism ; AMINES ; AMINO ACIDS ; ANIMAL CELLS ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; Binding Sites ; BIOCHEMISTRY ; Biological and medical sciences ; BIOSYNTHESIS ; CARBOHYDRATES ; CARBON 14 COMPOUNDS ; CARBOXYLIC ACIDS ; CELL CONSTITUENTS ; Cell Line ; Cell lines ; Cell Membrane - metabolism ; CELL MEMBRANES ; Cell membranes. Ionic channels. Membrane pores ; Cell structures and functions ; CHEMISTRY ; CHROMATOGRAPHY ; DAYS LIVING RADIOISOTOPES ; DRUGS ; ELECTROPHORESIS ; ENZYMES ; ESTERASES ; EVEN-ODD NUCLEI ; Fatty acids ; FETAL MEMBRANES ; Functional groups ; Fundamental and applied biological sciences. Psychology ; GLYCOLIPIDS ; HeLa cells ; Humans ; HYBRIDIZATION ; HYDROLASES ; INOSITOLS ; ISOTOPES ; LABELLED COMPOUNDS ; LIGHT NUCLEI ; LIPIDS ; LIPOTROPIC FACTORS ; LIQUID COLUMN CHROMATOGRAPHY ; man ; MEMBRANES ; MESSENGER-RNA ; METHIONINE ; Molecular and cellular biology ; MONOCARBOXYLIC ACIDS ; MONOSACCHARIDES ; NUCLEI ; NUCLEIC ACIDS ; ORGANIC ACIDS ; ORGANIC COMPOUNDS ; ORGANIC SULFUR COMPOUNDS ; PHOSPHATASES ; Phosphatidylinositol Diacylglycerol-Lyase ; Phosphoinositide Phospholipase C ; Phosphoric Diester Hydrolases - metabolism ; PLACENTA ; Placenta - metabolism ; Proteins ; Radioactive decay ; RADIOISOTOPES ; RNA ; SACCHARIDES ; SEPARATION PROCESSES ; SOMATIC CELLS ; SULFUR 35 ; SULFUR ISOTOPES ; SYNTHESIS ; TETRADECANOIC ACID ; TRITIUM COMPOUNDS</subject><ispartof>Proc. Natl. Acad. Sci. 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Natl. Acad. Sci. U.S.A.; (United States)</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] is a member of a diverse group of membrane proteins whose attachment to the lipid bilayer is mediated by a phosphatidylinositol-glycan. To investigate structural aspects of the glycolipid anchor, cultured WISH cells were used because we found that they produce the enzyme in abundant quantities. When cell suspensions were incubated with purified phosphatidylinositol-specific phospholipase C, most of the placental alkaline phosphatase was released from membranes in a hydrophilic form. On incubation of the cells with [14C]ethanolamine, [14C]myristic acid, or myo[3H]inositol, each was incorporated into the phosphatase near the carboxyl terminus, showing that these components, which are found in other phosphatidylinositol membrane-linked proteins, are also present in placental alkaline phosphatase.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>ALKALINE PHOSPHATASE</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>AMINES</subject><subject>AMINO ACIDS</subject><subject>ANIMAL CELLS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>Binding Sites</subject><subject>BIOCHEMISTRY</subject><subject>Biological and medical sciences</subject><subject>BIOSYNTHESIS</subject><subject>CARBOHYDRATES</subject><subject>CARBON 14 COMPOUNDS</subject><subject>CARBOXYLIC ACIDS</subject><subject>CELL CONSTITUENTS</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell Membrane - metabolism</subject><subject>CELL MEMBRANES</subject><subject>Cell membranes. Ionic channels. Membrane pores</subject><subject>Cell structures and functions</subject><subject>CHEMISTRY</subject><subject>CHROMATOGRAPHY</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>DRUGS</subject><subject>ELECTROPHORESIS</subject><subject>ENZYMES</subject><subject>ESTERASES</subject><subject>EVEN-ODD NUCLEI</subject><subject>Fatty acids</subject><subject>FETAL MEMBRANES</subject><subject>Functional groups</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLYCOLIPIDS</subject><subject>HeLa cells</subject><subject>Humans</subject><subject>HYBRIDIZATION</subject><subject>HYDROLASES</subject><subject>INOSITOLS</subject><subject>ISOTOPES</subject><subject>LABELLED COMPOUNDS</subject><subject>LIGHT NUCLEI</subject><subject>LIPIDS</subject><subject>LIPOTROPIC FACTORS</subject><subject>LIQUID COLUMN CHROMATOGRAPHY</subject><subject>man</subject><subject>MEMBRANES</subject><subject>MESSENGER-RNA</subject><subject>METHIONINE</subject><subject>Molecular and cellular biology</subject><subject>MONOCARBOXYLIC ACIDS</subject><subject>MONOSACCHARIDES</subject><subject>NUCLEI</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC SULFUR COMPOUNDS</subject><subject>PHOSPHATASES</subject><subject>Phosphatidylinositol Diacylglycerol-Lyase</subject><subject>Phosphoinositide Phospholipase C</subject><subject>Phosphoric Diester Hydrolases - metabolism</subject><subject>PLACENTA</subject><subject>Placenta - metabolism</subject><subject>Proteins</subject><subject>Radioactive decay</subject><subject>RADIOISOTOPES</subject><subject>RNA</subject><subject>SACCHARIDES</subject><subject>SEPARATION PROCESSES</subject><subject>SOMATIC CELLS</subject><subject>SULFUR 35</subject><subject>SULFUR ISOTOPES</subject><subject>SYNTHESIS</subject><subject>TETRADECANOIC ACID</subject><subject>TRITIUM COMPOUNDS</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2P0zAUxCMEWsrCGQkJFCEEp3TtxJ8HDlUFu0iL2AOcrRfHId514mK7iPLX46ilhQucLHl-897YUxRPMVpixJuLzQRxKcgS8yVDlN4rFhhJXDEi0f1igVDNK0Fq8rB4FOMtQkhSgc6Ks1pgKRhZFHfrAQLoZIL9Ccn6qfR9mQZT3gw-boZ81e2cnXy0ybvq0u00TOVHM7YBJlOuJj34MFuutmMWbhxoMyVw5crdQfad5kA0j4sHPbhonhzO8-LL-3ef11fV9afLD-vVdaUpEamCjmMhOO20qY2Udd8Ba4EwDCBFi1vOgJgadN8y0hnRcqo5pjXDzPTE0Lo5L97u52627Wi6OVEApzbBjhB2yoNVfyuTHdRX_13VUiLEsv_l3u9jsipqm4wetJ8mo5OimFOKRIZeH5YE_21rYlKjjdo4l__Fb6PinCNGGvpfEBOBEcYygxd7UAcfYzD9MTFGai5bzWUrQRTmai47O57_-dAjf2g3668OOkQNrs-daRuPGG-4JHjG3hywef5v9bRH9VvnkvmRMvnin2QGnu2B25h8OAWSnMnmF6m31k8</recordid><startdate>19870901</startdate><enddate>19870901</enddate><creator>Howard, Andrew D.</creator><creator>Berger, Joel</creator><creator>Gerber, Louise</creator><creator>Familletti, Philip</creator><creator>Udenfriend, Sidney</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19870901</creationdate><title>Characterization of the Phosphatidylinositol-Glycan Membrane Anchor of Human Placental Alkaline Phosphatase</title><author>Howard, Andrew D. ; Berger, Joel ; Gerber, Louise ; Familletti, Philip ; Udenfriend, Sidney</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-ad718875dce2e992fda6ba461aa98b1b76a4e2acfb64de8b75c7152616ef4e523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>ALKALINE PHOSPHATASE</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>AMINES</topic><topic>AMINO ACIDS</topic><topic>ANIMAL CELLS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>Binding Sites</topic><topic>BIOCHEMISTRY</topic><topic>Biological and medical sciences</topic><topic>BIOSYNTHESIS</topic><topic>CARBOHYDRATES</topic><topic>CARBON 14 COMPOUNDS</topic><topic>CARBOXYLIC ACIDS</topic><topic>CELL CONSTITUENTS</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell Membrane - metabolism</topic><topic>CELL MEMBRANES</topic><topic>Cell membranes. Ionic channels. Membrane pores</topic><topic>Cell structures and functions</topic><topic>CHEMISTRY</topic><topic>CHROMATOGRAPHY</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>DRUGS</topic><topic>ELECTROPHORESIS</topic><topic>ENZYMES</topic><topic>ESTERASES</topic><topic>EVEN-ODD NUCLEI</topic><topic>Fatty acids</topic><topic>FETAL MEMBRANES</topic><topic>Functional groups</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLYCOLIPIDS</topic><topic>HeLa cells</topic><topic>Humans</topic><topic>HYBRIDIZATION</topic><topic>HYDROLASES</topic><topic>INOSITOLS</topic><topic>ISOTOPES</topic><topic>LABELLED COMPOUNDS</topic><topic>LIGHT NUCLEI</topic><topic>LIPIDS</topic><topic>LIPOTROPIC FACTORS</topic><topic>LIQUID COLUMN CHROMATOGRAPHY</topic><topic>man</topic><topic>MEMBRANES</topic><topic>MESSENGER-RNA</topic><topic>METHIONINE</topic><topic>Molecular and cellular biology</topic><topic>MONOCARBOXYLIC ACIDS</topic><topic>MONOSACCHARIDES</topic><topic>NUCLEI</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC SULFUR COMPOUNDS</topic><topic>PHOSPHATASES</topic><topic>Phosphatidylinositol Diacylglycerol-Lyase</topic><topic>Phosphoinositide Phospholipase C</topic><topic>Phosphoric Diester Hydrolases - metabolism</topic><topic>PLACENTA</topic><topic>Placenta - metabolism</topic><topic>Proteins</topic><topic>Radioactive decay</topic><topic>RADIOISOTOPES</topic><topic>RNA</topic><topic>SACCHARIDES</topic><topic>SEPARATION PROCESSES</topic><topic>SOMATIC CELLS</topic><topic>SULFUR 35</topic><topic>SULFUR ISOTOPES</topic><topic>SYNTHESIS</topic><topic>TETRADECANOIC ACID</topic><topic>TRITIUM COMPOUNDS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Howard, Andrew D.</creatorcontrib><creatorcontrib>Berger, Joel</creatorcontrib><creatorcontrib>Gerber, Louise</creatorcontrib><creatorcontrib>Familletti, Philip</creatorcontrib><creatorcontrib>Udenfriend, Sidney</creatorcontrib><creatorcontrib>Roche Research Center, Nutley, NJ (USA)</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Howard, Andrew D.</au><au>Berger, Joel</au><au>Gerber, Louise</au><au>Familletti, Philip</au><au>Udenfriend, Sidney</au><aucorp>Roche Research Center, Nutley, NJ (USA)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the Phosphatidylinositol-Glycan Membrane Anchor of Human Placental Alkaline Phosphatase</atitle><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-09-01</date><risdate>1987</risdate><volume>84</volume><issue>17</issue><spage>6055</spage><epage>6059</epage><pages>6055-6059</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] is a member of a diverse group of membrane proteins whose attachment to the lipid bilayer is mediated by a phosphatidylinositol-glycan. To investigate structural aspects of the glycolipid anchor, cultured WISH cells were used because we found that they produce the enzyme in abundant quantities. When cell suspensions were incubated with purified phosphatidylinositol-specific phospholipase C, most of the placental alkaline phosphatase was released from membranes in a hydrophilic form. On incubation of the cells with [14C]ethanolamine, [14C]myristic acid, or myo[3H]inositol, each was incorporated into the phosphatase near the carboxyl terminus, showing that these components, which are found in other phosphatidylinositol membrane-linked proteins, are also present in placental alkaline phosphatase.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2819864</pmid><doi>10.1073/pnas.84.17.6055</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proc. Natl. Acad. Sci. U.S.A.; (United States), 1987-09, Vol.84 (17), p.6055-6059 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77706435 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 550201 - Biochemistry- Tracer Techniques ALKALINE PHOSPHATASE Alkaline Phosphatase - metabolism AMINES AMINO ACIDS ANIMAL CELLS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Binding Sites BIOCHEMISTRY Biological and medical sciences BIOSYNTHESIS CARBOHYDRATES CARBON 14 COMPOUNDS CARBOXYLIC ACIDS CELL CONSTITUENTS Cell Line Cell lines Cell Membrane - metabolism CELL MEMBRANES Cell membranes. Ionic channels. Membrane pores Cell structures and functions CHEMISTRY CHROMATOGRAPHY DAYS LIVING RADIOISOTOPES DRUGS ELECTROPHORESIS ENZYMES ESTERASES EVEN-ODD NUCLEI Fatty acids FETAL MEMBRANES Functional groups Fundamental and applied biological sciences. Psychology GLYCOLIPIDS HeLa cells Humans HYBRIDIZATION HYDROLASES INOSITOLS ISOTOPES LABELLED COMPOUNDS LIGHT NUCLEI LIPIDS LIPOTROPIC FACTORS LIQUID COLUMN CHROMATOGRAPHY man MEMBRANES MESSENGER-RNA METHIONINE Molecular and cellular biology MONOCARBOXYLIC ACIDS MONOSACCHARIDES NUCLEI NUCLEIC ACIDS ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS PHOSPHATASES Phosphatidylinositol Diacylglycerol-Lyase Phosphoinositide Phospholipase C Phosphoric Diester Hydrolases - metabolism PLACENTA Placenta - metabolism Proteins Radioactive decay RADIOISOTOPES RNA SACCHARIDES SEPARATION PROCESSES SOMATIC CELLS SULFUR 35 SULFUR ISOTOPES SYNTHESIS TETRADECANOIC ACID TRITIUM COMPOUNDS |
| title | Characterization of the Phosphatidylinositol-Glycan Membrane Anchor of Human Placental Alkaline Phosphatase |
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