Chromosomal Mapping, Isolation, and Characterization of the Mouse Xanthine Dehydrogenase Gene
Xanthine dehydrogenase (XD) is a key enzyme in the catabolism of purines. A recently isolated XD cDNA clone (Terao et al, Biochem. J. 283, 863-870, 1992) was used to analyze the genomic structure and chromosomal location of this gene. XD was found to be a single-copy gene approximately 70 kb long wi...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1994-09, Vol.23 (2), p.390-402 |
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| creator | Cazzaniga, Giovanni Terao, Mineko Lo Schiavo, Pietro Galbiati, Ferruccio Segalla, Fabio Seldin, Michael F. Garattini, Enrico |
| description | Xanthine dehydrogenase (XD) is a key enzyme in the catabolism of purines. A recently isolated XD cDNA clone (Terao
et al, Biochem. J. 283, 863-870, 1992) was used to analyze the genomic structure and chromosomal location of this gene. XD was found to be a single-copy gene approximately 70 kb long with 36 exons containing the transcribed sequence. The length of the mouse XD gene was much longer and the structure more complex than those of the
Drosophila and
Calliphora homologs. The locus encoding the XD gene (designated
Xd) was mapped to the distal part of mouse chromosome 17 by haplotype analysis of 114 interspecific backcross mice. Although
Xd inactivation may be responsible for xanthinuria, a rare human genetic disease, this genetic locus is not a candidate for any previously described mouse mutation. The transcription start site was defined by primer extension and RNase mapping analysis, using liver mRNA. No other transcription start sites were identified in the liver and a variety of other organs after treatment with an interferon inducer. Transient transfection analysis in NIH3T3, tend, and COS cells with an appropriate reporter gene demonstrated that a functional promoter is located within the first 268 bp preceding the transcriptional initiation site. |
| doi_str_mv | 10.1006/geno.1994.1515 |
| format | Article |
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et al, Biochem. J. 283, 863-870, 1992) was used to analyze the genomic structure and chromosomal location of this gene. XD was found to be a single-copy gene approximately 70 kb long with 36 exons containing the transcribed sequence. The length of the mouse XD gene was much longer and the structure more complex than those of the
Drosophila and
Calliphora homologs. The locus encoding the XD gene (designated
Xd) was mapped to the distal part of mouse chromosome 17 by haplotype analysis of 114 interspecific backcross mice. Although
Xd inactivation may be responsible for xanthinuria, a rare human genetic disease, this genetic locus is not a candidate for any previously described mouse mutation. The transcription start site was defined by primer extension and RNase mapping analysis, using liver mRNA. No other transcription start sites were identified in the liver and a variety of other organs after treatment with an interferon inducer. Transient transfection analysis in NIH3T3, tend, and COS cells with an appropriate reporter gene demonstrated that a functional promoter is located within the first 268 bp preceding the transcriptional initiation site.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1994.1515</identifier><identifier>PMID: 7835888</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Chromosome Mapping ; Cloning, Molecular ; Crosses, Genetic ; Diptera - enzymology ; Diptera - genetics ; DNA Primers - genetics ; DNA, Complementary - genetics ; Exons ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Genetic Markers ; Humans ; Introns ; Mice ; Mice, Inbred C3H ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Muridae ; Xanthine Dehydrogenase - genetics</subject><ispartof>Genomics (San Diego, Calif.), 1994-09, Vol.23 (2), p.390-402</ispartof><rights>1994 Academic Press</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-4a4c357b80ea17e6e36ba939331f39f1c4922bc87f83df40c54ae6ab8369823a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754384715151$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3307020$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7835888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cazzaniga, Giovanni</creatorcontrib><creatorcontrib>Terao, Mineko</creatorcontrib><creatorcontrib>Lo Schiavo, Pietro</creatorcontrib><creatorcontrib>Galbiati, Ferruccio</creatorcontrib><creatorcontrib>Segalla, Fabio</creatorcontrib><creatorcontrib>Seldin, Michael F.</creatorcontrib><creatorcontrib>Garattini, Enrico</creatorcontrib><title>Chromosomal Mapping, Isolation, and Characterization of the Mouse Xanthine Dehydrogenase Gene</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Xanthine dehydrogenase (XD) is a key enzyme in the catabolism of purines. A recently isolated XD cDNA clone (Terao
et al, Biochem. J. 283, 863-870, 1992) was used to analyze the genomic structure and chromosomal location of this gene. XD was found to be a single-copy gene approximately 70 kb long with 36 exons containing the transcribed sequence. The length of the mouse XD gene was much longer and the structure more complex than those of the
Drosophila and
Calliphora homologs. The locus encoding the XD gene (designated
Xd) was mapped to the distal part of mouse chromosome 17 by haplotype analysis of 114 interspecific backcross mice. Although
Xd inactivation may be responsible for xanthinuria, a rare human genetic disease, this genetic locus is not a candidate for any previously described mouse mutation. The transcription start site was defined by primer extension and RNase mapping analysis, using liver mRNA. No other transcription start sites were identified in the liver and a variety of other organs after treatment with an interferon inducer. Transient transfection analysis in NIH3T3, tend, and COS cells with an appropriate reporter gene demonstrated that a functional promoter is located within the first 268 bp preceding the transcriptional initiation site.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>Crosses, Genetic</subject><subject>Diptera - enzymology</subject><subject>Diptera - genetics</subject><subject>DNA Primers - genetics</subject><subject>DNA, Complementary - genetics</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Genetic Markers</subject><subject>Humans</subject><subject>Introns</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Muridae</subject><subject>Xanthine Dehydrogenase - genetics</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1r3DAQhkVpSDdpr70VdCg9xVvJsvVxLJs2CST00kIvRYzlcaxiS1vJG0h_fbXZJbecBuZ9Znh5CHnP2ZozJj_fY4hrbkyz5i1vX5EVZ9pUWjbyNVkxrXWl2ka8IWc5_2GMGaHrU3KqtGhLtiK_N2OKc8xxhonewXbrw_0FvclxgsXHcEEh9HQzQgK3YPL_nrY0DnQZkd7FXUb6C8Iy-oD0EsfHPsXSCMr6CgO-JScDTBnfHec5-fnt64_NdXX7_epm8-W2ckLqpWqgcaJVnWYIXKFEITswwgjBB2EG7hpT153TatCiHxrm2gZQQqeFNLoWIM7Jp8PfbYp_d5gXO_vscJogYOlolVJMCm0KuD6ALsWcEw52m_wM6dFyZvc-7d6n3fu0e5_l4MPx866bsX_GjwJL_vGYQ3YwDQmC8_kZE4IpVrOC6QOGxcKDx2Sz8xgc9j6hW2wf_UsN_gPGJZF0</recordid><startdate>19940915</startdate><enddate>19940915</enddate><creator>Cazzaniga, Giovanni</creator><creator>Terao, Mineko</creator><creator>Lo Schiavo, Pietro</creator><creator>Galbiati, Ferruccio</creator><creator>Segalla, Fabio</creator><creator>Seldin, Michael F.</creator><creator>Garattini, Enrico</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940915</creationdate><title>Chromosomal Mapping, Isolation, and Characterization of the Mouse Xanthine Dehydrogenase Gene</title><author>Cazzaniga, Giovanni ; Terao, Mineko ; Lo Schiavo, Pietro ; Galbiati, Ferruccio ; Segalla, Fabio ; Seldin, Michael F. ; Garattini, Enrico</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-4a4c357b80ea17e6e36ba939331f39f1c4922bc87f83df40c54ae6ab8369823a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>Crosses, Genetic</topic><topic>Diptera - enzymology</topic><topic>Diptera - genetics</topic><topic>DNA Primers - genetics</topic><topic>DNA, Complementary - genetics</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Genetic Markers</topic><topic>Humans</topic><topic>Introns</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Muridae</topic><topic>Xanthine Dehydrogenase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cazzaniga, Giovanni</creatorcontrib><creatorcontrib>Terao, Mineko</creatorcontrib><creatorcontrib>Lo Schiavo, Pietro</creatorcontrib><creatorcontrib>Galbiati, Ferruccio</creatorcontrib><creatorcontrib>Segalla, Fabio</creatorcontrib><creatorcontrib>Seldin, Michael F.</creatorcontrib><creatorcontrib>Garattini, Enrico</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cazzaniga, Giovanni</au><au>Terao, Mineko</au><au>Lo Schiavo, Pietro</au><au>Galbiati, Ferruccio</au><au>Segalla, Fabio</au><au>Seldin, Michael F.</au><au>Garattini, Enrico</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromosomal Mapping, Isolation, and Characterization of the Mouse Xanthine Dehydrogenase Gene</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1994-09-15</date><risdate>1994</risdate><volume>23</volume><issue>2</issue><spage>390</spage><epage>402</epage><pages>390-402</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Xanthine dehydrogenase (XD) is a key enzyme in the catabolism of purines. A recently isolated XD cDNA clone (Terao
et al, Biochem. J. 283, 863-870, 1992) was used to analyze the genomic structure and chromosomal location of this gene. XD was found to be a single-copy gene approximately 70 kb long with 36 exons containing the transcribed sequence. The length of the mouse XD gene was much longer and the structure more complex than those of the
Drosophila and
Calliphora homologs. The locus encoding the XD gene (designated
Xd) was mapped to the distal part of mouse chromosome 17 by haplotype analysis of 114 interspecific backcross mice. Although
Xd inactivation may be responsible for xanthinuria, a rare human genetic disease, this genetic locus is not a candidate for any previously described mouse mutation. The transcription start site was defined by primer extension and RNase mapping analysis, using liver mRNA. No other transcription start sites were identified in the liver and a variety of other organs after treatment with an interferon inducer. Transient transfection analysis in NIH3T3, tend, and COS cells with an appropriate reporter gene demonstrated that a functional promoter is located within the first 268 bp preceding the transcriptional initiation site.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>7835888</pmid><doi>10.1006/geno.1994.1515</doi><tpages>13</tpages></addata></record> |
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| ispartof | Genomics (San Diego, Calif.), 1994-09, Vol.23 (2), p.390-402 |
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| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Chromosome Mapping Cloning, Molecular Crosses, Genetic Diptera - enzymology Diptera - genetics DNA Primers - genetics DNA, Complementary - genetics Exons Fundamental and applied biological sciences. Psychology Genes. Genome Genetic Markers Humans Introns Mice Mice, Inbred C3H Molecular and cellular biology Molecular genetics Molecular Sequence Data Muridae Xanthine Dehydrogenase - genetics |
| title | Chromosomal Mapping, Isolation, and Characterization of the Mouse Xanthine Dehydrogenase Gene |
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