Extensive diversity in repeat unit sequences of the cDNA encoding the polyprotein antigen/allergen from the bovine lungworm Dictyocaulus viviparus
The complete sequence of the cDNA encoding the nematode polyprotein allergen/antigen (NPA) of the bovine lungworm Dictyocaulus viviparus was obtained by immunoscreening of cDNA expression libraries and by 5′ RACE (rapid amplification of cDNA ends). The encoded polypeptide is similar in sequence to t...
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description | The complete sequence of the cDNA encoding the nematode polyprotein allergen/antigen (NPA) of the bovine lungworm
Dictyocaulus viviparus was obtained by immunoscreening of cDNA expression libraries and by 5′ RACE (rapid amplification of cDNA ends). The encoded polypeptide is similar in sequence to the ABA-1 allergen of
Ascaris, the gp15/400 ‘ladder’ protein of
Brugia malayi, Brugia pahangi and
Wuchereria bancrofti, and a 15-kDa antigen of
Dirofilaria immitis. As with these, the predicted amino-acid sequence comprises a head-to-tail array of similar polypeptides with regularly spaced consensus proteinase cleavage sites. The
D. viviparus protein was designated DvA-1 (
D. viviparus antigen-1) and the gene
dva-1. The deduced amino-acid sequence of DvA-1 showed features not observed before in other NPAs: (
i) a hydrophobic leader peptide is present, (
ii) none of the 12 units in the array are indentical and the sequences diverge to a degree hitherto unseen in the NPAs of other nematode parasites, (
iii) the predicted proteinase cleavage sites are also diverse in sequence and, in two instances, no consensus cleavage site was identifiable at the expected position, (
iv) a short repeat unit is present, which is the only one containing a consensus
N-glycosylation site and (
v) a C-terminal extension peptide is encoded which shows no similarity to that from
A. suum ABA-1. Comparison of independent cDNAs revealed slight variations in the sequence of the gene within the parasite population. Antisera to recombinant DvA-1 polypeptide identified 14–15-kDa antigens in both parasite somatic and excretory-secretory material. DvA-1 is the only NPA for which the complete coding sequence is available and the new principles which it illustrates may lie unsuspected in the NPA-encoding genes of all nematode parasites. |
doi_str_mv | 10.1016/0166-6851(95)00088-I |
format | Article |
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Dictyocaulus viviparus was obtained by immunoscreening of cDNA expression libraries and by 5′ RACE (rapid amplification of cDNA ends). The encoded polypeptide is similar in sequence to the ABA-1 allergen of
Ascaris, the gp15/400 ‘ladder’ protein of
Brugia malayi, Brugia pahangi and
Wuchereria bancrofti, and a 15-kDa antigen of
Dirofilaria immitis. As with these, the predicted amino-acid sequence comprises a head-to-tail array of similar polypeptides with regularly spaced consensus proteinase cleavage sites. The
D. viviparus protein was designated DvA-1 (
D. viviparus antigen-1) and the gene
dva-1. The deduced amino-acid sequence of DvA-1 showed features not observed before in other NPAs: (
i) a hydrophobic leader peptide is present, (
ii) none of the 12 units in the array are indentical and the sequences diverge to a degree hitherto unseen in the NPAs of other nematode parasites, (
iii) the predicted proteinase cleavage sites are also diverse in sequence and, in two instances, no consensus cleavage site was identifiable at the expected position, (
iv) a short repeat unit is present, which is the only one containing a consensus
N-glycosylation site and (
v) a C-terminal extension peptide is encoded which shows no similarity to that from
A. suum ABA-1. Comparison of independent cDNAs revealed slight variations in the sequence of the gene within the parasite population. Antisera to recombinant DvA-1 polypeptide identified 14–15-kDa antigens in both parasite somatic and excretory-secretory material. DvA-1 is the only NPA for which the complete coding sequence is available and the new principles which it illustrates may lie unsuspected in the NPA-encoding genes of all nematode parasites.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/0166-6851(95)00088-I</identifier><identifier>PMID: 8538702</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animals ; antigens ; Antigens, Helminth ; Base Sequence ; Cattle ; cloning ; complementary DNA ; Dictyocaulus - genetics ; Dictyocaulus - immunology ; Dictyocaulus viviparus ; DNA, Complementary - genetics ; DNA, Helminth - genetics ; dva-1 gene ; Excretory-secretory antigen ; Fatty Acid-Binding Proteins ; genbank/u02568 ; gene expression ; Gene Library ; Guinea Pigs ; Helminth Proteins - genetics ; Helminth Proteins - immunology ; Helminths - genetics ; heterogeneity ; immunochemistry ; lungworms ; Molecular Sequence Data ; Nematode ; Nematode polyprotein allergen/antigen ; nucleotide sequences ; Passive Cutaneous Anaphylaxis ; proteinase cleavage sites ; proteolysis ; Repetitive Sequences, Nucleic Acid ; RNA, Helminth - genetics ; RNA, Messenger - genetics ; Sequence Alignment ; Sequence Homology, Amino Acid ; Species Specificity ; structural genes</subject><ispartof>Molecular and biochemical parasitology, 1995-06, Vol.72 (1), p.77-88</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-43519d03ed3547db0570dd3a2306fd31ea28c387647aeeb39d4947e4a1c36df63</citedby><cites>FETCH-LOGICAL-c412t-43519d03ed3547db0570dd3a2306fd31ea28c387647aeeb39d4947e4a1c36df63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-6851(95)00088-I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8538702$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Britton, Collette</creatorcontrib><creatorcontrib>Moore, Joyce</creatorcontrib><creatorcontrib>Gilleard, John S.</creatorcontrib><creatorcontrib>Kennedy, Malcolm W.</creatorcontrib><title>Extensive diversity in repeat unit sequences of the cDNA encoding the polyprotein antigen/allergen from the bovine lungworm Dictyocaulus viviparus</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>The complete sequence of the cDNA encoding the nematode polyprotein allergen/antigen (NPA) of the bovine lungworm
Dictyocaulus viviparus was obtained by immunoscreening of cDNA expression libraries and by 5′ RACE (rapid amplification of cDNA ends). The encoded polypeptide is similar in sequence to the ABA-1 allergen of
Ascaris, the gp15/400 ‘ladder’ protein of
Brugia malayi, Brugia pahangi and
Wuchereria bancrofti, and a 15-kDa antigen of
Dirofilaria immitis. As with these, the predicted amino-acid sequence comprises a head-to-tail array of similar polypeptides with regularly spaced consensus proteinase cleavage sites. The
D. viviparus protein was designated DvA-1 (
D. viviparus antigen-1) and the gene
dva-1. The deduced amino-acid sequence of DvA-1 showed features not observed before in other NPAs: (
i) a hydrophobic leader peptide is present, (
ii) none of the 12 units in the array are indentical and the sequences diverge to a degree hitherto unseen in the NPAs of other nematode parasites, (
iii) the predicted proteinase cleavage sites are also diverse in sequence and, in two instances, no consensus cleavage site was identifiable at the expected position, (
iv) a short repeat unit is present, which is the only one containing a consensus
N-glycosylation site and (
v) a C-terminal extension peptide is encoded which shows no similarity to that from
A. suum ABA-1. Comparison of independent cDNAs revealed slight variations in the sequence of the gene within the parasite population. Antisera to recombinant DvA-1 polypeptide identified 14–15-kDa antigens in both parasite somatic and excretory-secretory material. DvA-1 is the only NPA for which the complete coding sequence is available and the new principles which it illustrates may lie unsuspected in the NPA-encoding genes of all nematode parasites.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>antigens</subject><subject>Antigens, Helminth</subject><subject>Base Sequence</subject><subject>Cattle</subject><subject>cloning</subject><subject>complementary DNA</subject><subject>Dictyocaulus - genetics</subject><subject>Dictyocaulus - immunology</subject><subject>Dictyocaulus viviparus</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Helminth - genetics</subject><subject>dva-1 gene</subject><subject>Excretory-secretory antigen</subject><subject>Fatty Acid-Binding Proteins</subject><subject>genbank/u02568</subject><subject>gene expression</subject><subject>Gene Library</subject><subject>Guinea Pigs</subject><subject>Helminth Proteins - genetics</subject><subject>Helminth Proteins - immunology</subject><subject>Helminths - genetics</subject><subject>heterogeneity</subject><subject>immunochemistry</subject><subject>lungworms</subject><subject>Molecular Sequence Data</subject><subject>Nematode</subject><subject>Nematode polyprotein allergen/antigen</subject><subject>nucleotide sequences</subject><subject>Passive Cutaneous Anaphylaxis</subject><subject>proteinase cleavage sites</subject><subject>proteolysis</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RNA, Helminth - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Species Specificity</subject><subject>structural genes</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EKkvhH4DwCcEh1I4_4lwqVW2BlSo4QM-W154sRkkcbCewf4NfjPdDPcLB9mjmeUczfhF6Scl7Sqi8KEdWUgn6thXvCCFKVetHaEVVU1ctr9VjtHpAnqJnKf0okGikPENnSjDVkHqF_tz-zjAmvwB25YrJ5x32I44wgcl4Hn3GCX7OMFpIOHQ4fwdsbz5f4ZIJzo_bQ2YK_W6KIUORmjH7LYwXpu8hlgB3MQwHahMWPwLu53H7K8QB33ibd8GauZ8TXvziJxPn9Bw96Uyf4MXpPUf3H26_XX-q7r58XF9f3VWW0zpXnAnaOsLAMcEbtymrEeeYqRmRnWMUTK1s2VLyxgBsWOt4yxvghlomXSfZOXpz7FsGLwumrAefLPS9GSHMSTdNQ0TN-X9BKqSiiokC8iNoY0gpQqen6AcTd5oSvfdM7w3Re0N0K_TBM70uslen_vNmAPcgOplU6q-P9c4EbbbRJ33_tSaUESooV1IV4vJIQPmvxUPUyfq9Zc5HsFm74P89wl-IfrJt</recordid><startdate>19950601</startdate><enddate>19950601</enddate><creator>Britton, Collette</creator><creator>Moore, Joyce</creator><creator>Gilleard, John S.</creator><creator>Kennedy, Malcolm W.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19950601</creationdate><title>Extensive diversity in repeat unit sequences of the cDNA encoding the polyprotein antigen/allergen from the bovine lungworm Dictyocaulus viviparus</title><author>Britton, Collette ; Moore, Joyce ; Gilleard, John S. ; Kennedy, Malcolm W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-43519d03ed3547db0570dd3a2306fd31ea28c387647aeeb39d4947e4a1c36df63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>antigens</topic><topic>Antigens, Helminth</topic><topic>Base Sequence</topic><topic>Cattle</topic><topic>cloning</topic><topic>complementary DNA</topic><topic>Dictyocaulus - genetics</topic><topic>Dictyocaulus - immunology</topic><topic>Dictyocaulus viviparus</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Helminth - genetics</topic><topic>dva-1 gene</topic><topic>Excretory-secretory antigen</topic><topic>Fatty Acid-Binding Proteins</topic><topic>genbank/u02568</topic><topic>gene expression</topic><topic>Gene Library</topic><topic>Guinea Pigs</topic><topic>Helminth Proteins - genetics</topic><topic>Helminth Proteins - immunology</topic><topic>Helminths - genetics</topic><topic>heterogeneity</topic><topic>immunochemistry</topic><topic>lungworms</topic><topic>Molecular Sequence Data</topic><topic>Nematode</topic><topic>Nematode polyprotein allergen/antigen</topic><topic>nucleotide sequences</topic><topic>Passive Cutaneous Anaphylaxis</topic><topic>proteinase cleavage sites</topic><topic>proteolysis</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>RNA, Helminth - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Species Specificity</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Britton, Collette</creatorcontrib><creatorcontrib>Moore, Joyce</creatorcontrib><creatorcontrib>Gilleard, John S.</creatorcontrib><creatorcontrib>Kennedy, Malcolm W.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Britton, Collette</au><au>Moore, Joyce</au><au>Gilleard, John S.</au><au>Kennedy, Malcolm W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extensive diversity in repeat unit sequences of the cDNA encoding the polyprotein antigen/allergen from the bovine lungworm Dictyocaulus viviparus</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1995-06-01</date><risdate>1995</risdate><volume>72</volume><issue>1</issue><spage>77</spage><epage>88</epage><pages>77-88</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><abstract>The complete sequence of the cDNA encoding the nematode polyprotein allergen/antigen (NPA) of the bovine lungworm
Dictyocaulus viviparus was obtained by immunoscreening of cDNA expression libraries and by 5′ RACE (rapid amplification of cDNA ends). The encoded polypeptide is similar in sequence to the ABA-1 allergen of
Ascaris, the gp15/400 ‘ladder’ protein of
Brugia malayi, Brugia pahangi and
Wuchereria bancrofti, and a 15-kDa antigen of
Dirofilaria immitis. As with these, the predicted amino-acid sequence comprises a head-to-tail array of similar polypeptides with regularly spaced consensus proteinase cleavage sites. The
D. viviparus protein was designated DvA-1 (
D. viviparus antigen-1) and the gene
dva-1. The deduced amino-acid sequence of DvA-1 showed features not observed before in other NPAs: (
i) a hydrophobic leader peptide is present, (
ii) none of the 12 units in the array are indentical and the sequences diverge to a degree hitherto unseen in the NPAs of other nematode parasites, (
iii) the predicted proteinase cleavage sites are also diverse in sequence and, in two instances, no consensus cleavage site was identifiable at the expected position, (
iv) a short repeat unit is present, which is the only one containing a consensus
N-glycosylation site and (
v) a C-terminal extension peptide is encoded which shows no similarity to that from
A. suum ABA-1. Comparison of independent cDNAs revealed slight variations in the sequence of the gene within the parasite population. Antisera to recombinant DvA-1 polypeptide identified 14–15-kDa antigens in both parasite somatic and excretory-secretory material. DvA-1 is the only NPA for which the complete coding sequence is available and the new principles which it illustrates may lie unsuspected in the NPA-encoding genes of all nematode parasites.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8538702</pmid><doi>10.1016/0166-6851(95)00088-I</doi><tpages>12</tpages></addata></record> |
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source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Amino Acid Sequence amino acid sequences Animals antigens Antigens, Helminth Base Sequence Cattle cloning complementary DNA Dictyocaulus - genetics Dictyocaulus - immunology Dictyocaulus viviparus DNA, Complementary - genetics DNA, Helminth - genetics dva-1 gene Excretory-secretory antigen Fatty Acid-Binding Proteins genbank/u02568 gene expression Gene Library Guinea Pigs Helminth Proteins - genetics Helminth Proteins - immunology Helminths - genetics heterogeneity immunochemistry lungworms Molecular Sequence Data Nematode Nematode polyprotein allergen/antigen nucleotide sequences Passive Cutaneous Anaphylaxis proteinase cleavage sites proteolysis Repetitive Sequences, Nucleic Acid RNA, Helminth - genetics RNA, Messenger - genetics Sequence Alignment Sequence Homology, Amino Acid Species Specificity structural genes |
title | Extensive diversity in repeat unit sequences of the cDNA encoding the polyprotein antigen/allergen from the bovine lungworm Dictyocaulus viviparus |
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