A novel Escherichia coli lipoprotein expression vector
A novel Escherichia coli (Ec) lipoprotein expression plasmid, pSJLP, was constructed. The plasmid contains a truncated alkaline phosphatase gene (phoA) located downstream from the Lac repressor gene lacI q and the IPTG inducible p tac promoter. The phoA gene was truncated by deleting the native phoA...
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| Veröffentlicht in: | Gene 1995, Vol.165 (1), p.145-146 |
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| container_title | Gene |
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| creator | Jones, Thomas S. Tryon, Victor V. |
| description | A novel
Escherichia coli (Ec) lipoprotein expression plasmid, pSJLP, was constructed. The plasmid contains a truncated alkaline phosphatase gene
(phoA) located downstream from the Lac repressor gene
lacI
q
and the IPTG inducible
p
tac
promoter. The
phoA gene was truncated by deleting the native
phoA signal sequence and fusing the truncated
phoA gene to the lipoprotein signal sequence of the major
Ec lipoprotein LPP. The recombinant LPP::PhoA fusion protein is produced and processed as a lipoprotein and can therefore be used as substrate for a novel signal peptidase II assay. |
| doi_str_mv | 10.1016/0378-1119(95)00496-S |
| format | Article |
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Escherichia coli (Ec) lipoprotein expression plasmid, pSJLP, was constructed. The plasmid contains a truncated alkaline phosphatase gene
(phoA) located downstream from the Lac repressor gene
lacI
q
and the IPTG inducible
p
tac
promoter. The
phoA gene was truncated by deleting the native
phoA signal sequence and fusing the truncated
phoA gene to the lipoprotein signal sequence of the major
Ec lipoprotein LPP. The recombinant LPP::PhoA fusion protein is produced and processed as a lipoprotein and can therefore be used as substrate for a novel signal peptidase II assay.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(95)00496-S</identifier><identifier>PMID: 7489907</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alkaline phosphatase ; Base Sequence ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Genetic Vectors ; Lipoproteins - genetics ; Molecular Sequence Data ; Plasmids - genetics ; Plasmids - isolation & purification ; protein export ; signal peptidase II ; signal sequence</subject><ispartof>Gene, 1995, Vol.165 (1), p.145-146</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c404t-380983289d6fc8d7639ab082ff06cd9139ae806d1ab8c81a7b75bd796a9b1c6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(95)00496-S$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7489907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jones, Thomas S.</creatorcontrib><creatorcontrib>Tryon, Victor V.</creatorcontrib><title>A novel Escherichia coli lipoprotein expression vector</title><title>Gene</title><addtitle>Gene</addtitle><description>A novel
Escherichia coli (Ec) lipoprotein expression plasmid, pSJLP, was constructed. The plasmid contains a truncated alkaline phosphatase gene
(phoA) located downstream from the Lac repressor gene
lacI
q
and the IPTG inducible
p
tac
promoter. The
phoA gene was truncated by deleting the native
phoA signal sequence and fusing the truncated
phoA gene to the lipoprotein signal sequence of the major
Ec lipoprotein LPP. The recombinant LPP::PhoA fusion protein is produced and processed as a lipoprotein and can therefore be used as substrate for a novel signal peptidase II assay.</description><subject>Alkaline phosphatase</subject><subject>Base Sequence</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Genetic Vectors</subject><subject>Lipoproteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Plasmids - genetics</subject><subject>Plasmids - isolation & purification</subject><subject>protein export</subject><subject>signal peptidase II</subject><subject>signal sequence</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtLAzEQgIMotT7-gcKeRA-rk33kcRFKqQ8oeKiew252lka2mzXZFv33Zm3pUZ1LGOabmfANIRcUbilQdgcpFzGlVF7L_AYgkyxeHJAxFVzGAKk4JOM9ckxOvH-HEHmejMiIZ0JK4GPCJlFrN9hEM6-X6IxemiLStjFRYzrbOdujaSP87Bx6b2wbbVD31p2Ro7poPJ7v3lPy9jB7nT7F85fH5-lkHusMsj5OBUiRJkJWrNai4iyVRQkiqWtgupI0pCiAVbQohRa04CXPy4pLVsiSalamp-RqOzf85GONvlcr4zU2TdGiXXvFOYdEsvRPkA5clrP_gJBxQQOYbUHtrPcOa9U5syrcl6KghgOowa4a7CqZq58DqEVou9zNX5crrPZNO-Ohfr-tY9C2MeiU1wZbjZVxQa2qrPl9wTcIUZPu</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Jones, Thomas S.</creator><creator>Tryon, Victor V.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>A novel Escherichia coli lipoprotein expression vector</title><author>Jones, Thomas S. ; Tryon, Victor V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-380983289d6fc8d7639ab082ff06cd9139ae806d1ab8c81a7b75bd796a9b1c6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Alkaline phosphatase</topic><topic>Base Sequence</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Genetic Vectors</topic><topic>Lipoproteins - genetics</topic><topic>Molecular Sequence Data</topic><topic>Plasmids - genetics</topic><topic>Plasmids - isolation & purification</topic><topic>protein export</topic><topic>signal peptidase II</topic><topic>signal sequence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jones, Thomas S.</creatorcontrib><creatorcontrib>Tryon, Victor V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jones, Thomas S.</au><au>Tryon, Victor V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel Escherichia coli lipoprotein expression vector</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1995</date><risdate>1995</risdate><volume>165</volume><issue>1</issue><spage>145</spage><epage>146</epage><pages>145-146</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A novel
Escherichia coli (Ec) lipoprotein expression plasmid, pSJLP, was constructed. The plasmid contains a truncated alkaline phosphatase gene
(phoA) located downstream from the Lac repressor gene
lacI
q
and the IPTG inducible
p
tac
promoter. The
phoA gene was truncated by deleting the native
phoA signal sequence and fusing the truncated
phoA gene to the lipoprotein signal sequence of the major
Ec lipoprotein LPP. The recombinant LPP::PhoA fusion protein is produced and processed as a lipoprotein and can therefore be used as substrate for a novel signal peptidase II assay.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7489907</pmid><doi>10.1016/0378-1119(95)00496-S</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1995, Vol.165 (1), p.145-146 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77702963 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alkaline phosphatase Base Sequence Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Genetic Vectors Lipoproteins - genetics Molecular Sequence Data Plasmids - genetics Plasmids - isolation & purification protein export signal peptidase II signal sequence |
| title | A novel Escherichia coli lipoprotein expression vector |
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