A Radiation Hybrid Map with 60 Loci Covering the Entire Short Arm of Chromosome 12
We present a high-resolution radiation hybrid map of the short arm of human chromosome 12 containing 60 loci, including 44 STSs within or closely associated with expressed sequences, 11 highly polymorphic markers, 2 anonymous sequences, 2 subtelomeric sequences, and 1 centromeric sequence. The 60 lo...
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| Veröffentlicht in: | Genomics 1995-09, Vol.29 (1), p.170-178 |
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| creator | Raeymaekers, Peter Van Zand, Karel Jun, Lin Höglund, Mattias Cassiman, Jean-Jacques Van den Berghe, Herman Marynen, Peter |
| description | We present a high-resolution radiation hybrid map of the short arm of human chromosome 12 containing 60 loci, including 44 STSs within or closely associated with expressed sequences, 11 highly polymorphic markers, 2 anonymous sequences, 2 subtelomeric sequences, and 1 centromeric sequence. The 60 loci fell into 48 unique retention patterns, providing a comprehensive map covering the entire short arm of chromosome 12 with an average resolution of approximately 800 kb. Twenty-two unique positions were ordered in a 1000:1 framework map with an average resolution of 1.8 Mb. The proposed order is in good agreement with recently published genetic maps, high-resolution FISH maps, and YAC contigs. The noted inconsistencies involved neighboring loci permutations. Our observations further suggest the existence of chromosomal "hot spots" for breakage during irradiation. In three regions an usually high number of breaks was noted between neighboring loci compared to the physical distance derived from existing YAC contigs. Some of these hot spots seem to coincide with known chromosomal aberrations, of which at least two have been involved in the etiology of cancer. |
| doi_str_mv | 10.1006/geno.1995.1228 |
| format | Article |
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The 60 loci fell into 48 unique retention patterns, providing a comprehensive map covering the entire short arm of chromosome 12 with an average resolution of approximately 800 kb. Twenty-two unique positions were ordered in a 1000:1 framework map with an average resolution of 1.8 Mb. The proposed order is in good agreement with recently published genetic maps, high-resolution FISH maps, and YAC contigs. The noted inconsistencies involved neighboring loci permutations. Our observations further suggest the existence of chromosomal "hot spots" for breakage during irradiation. In three regions an usually high number of breaks was noted between neighboring loci compared to the physical distance derived from existing YAC contigs. Some of these hot spots seem to coincide with known chromosomal aberrations, of which at least two have been involved in the etiology of cancer.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1995.1228</identifier><identifier>PMID: 8530068</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; BIOLOGICAL MARKERS ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Cell Line ; Centromere ; CHROMOSOMAL ABERRATIONS ; Chromosome Aberrations ; Chromosome Mapping ; Chromosomes, Artificial, Yeast ; Chromosomes, Human, Pair 12 - radiation effects ; Classical genetics, quantitative genetics, hybrids ; CONTIGS ; Cricetinae ; Cricetulus ; DNA SEQUENCING ; ETIOLOGY ; Fundamental and applied biological sciences. Psychology ; GENE MUTATIONS ; GENES ; GENETIC MAPPING ; Genetic Markers ; GENETIC RADIATION EFFECTS ; Genetics of eukaryotes. Biological and molecular evolution ; Human ; HUMAN CHROMOSOME 12 ; Humans ; Hybrid Cells - radiation effects ; Hypoxanthine Phosphoribosyltransferase - deficiency ; Hypoxanthine Phosphoribosyltransferase - genetics ; In Situ Hybridization, Fluorescence ; Molecular Sequence Data ; NEOPLASMS ; Neoplasms - genetics ; Oligonucleotide Probes ; POLYMERASE CHAIN REACTION ; Polymorphism, Genetic ; RADIATION INDUCED MUTANTS ; RESOLUTION ; Telomere</subject><ispartof>Genomics, 1995-09, Vol.29 (1), p.170-178</ispartof><rights>1995 Academic Press</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-98f0d13d170effb2610e42a832043e1970c9df7a26da1d490572c1140e8596ec3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754385712281$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3659328$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8530068$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/433298$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Raeymaekers, Peter</creatorcontrib><creatorcontrib>Van Zand, Karel</creatorcontrib><creatorcontrib>Jun, Lin</creatorcontrib><creatorcontrib>Höglund, Mattias</creatorcontrib><creatorcontrib>Cassiman, Jean-Jacques</creatorcontrib><creatorcontrib>Van den Berghe, Herman</creatorcontrib><creatorcontrib>Marynen, Peter</creatorcontrib><title>A Radiation Hybrid Map with 60 Loci Covering the Entire Short Arm of Chromosome 12</title><title>Genomics</title><addtitle>Genomics</addtitle><description>We present a high-resolution radiation hybrid map of the short arm of human chromosome 12 containing 60 loci, including 44 STSs within or closely associated with expressed sequences, 11 highly polymorphic markers, 2 anonymous sequences, 2 subtelomeric sequences, and 1 centromeric sequence. The 60 loci fell into 48 unique retention patterns, providing a comprehensive map covering the entire short arm of chromosome 12 with an average resolution of approximately 800 kb. Twenty-two unique positions were ordered in a 1000:1 framework map with an average resolution of 1.8 Mb. The proposed order is in good agreement with recently published genetic maps, high-resolution FISH maps, and YAC contigs. The noted inconsistencies involved neighboring loci permutations. Our observations further suggest the existence of chromosomal "hot spots" for breakage during irradiation. In three regions an usually high number of breaks was noted between neighboring loci compared to the physical distance derived from existing YAC contigs. Some of these hot spots seem to coincide with known chromosomal aberrations, of which at least two have been involved in the etiology of cancer.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL MARKERS</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Cell Line</subject><subject>Centromere</subject><subject>CHROMOSOMAL ABERRATIONS</subject><subject>Chromosome Aberrations</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Artificial, Yeast</subject><subject>Chromosomes, Human, Pair 12 - radiation effects</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>CONTIGS</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>DNA SEQUENCING</subject><subject>ETIOLOGY</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE MUTATIONS</subject><subject>GENES</subject><subject>GENETIC MAPPING</subject><subject>Genetic Markers</subject><subject>GENETIC RADIATION EFFECTS</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Human</subject><subject>HUMAN CHROMOSOME 12</subject><subject>Humans</subject><subject>Hybrid Cells - radiation effects</subject><subject>Hypoxanthine Phosphoribosyltransferase - deficiency</subject><subject>Hypoxanthine Phosphoribosyltransferase - genetics</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Molecular Sequence Data</subject><subject>NEOPLASMS</subject><subject>Neoplasms - genetics</subject><subject>Oligonucleotide Probes</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>Polymorphism, Genetic</subject><subject>RADIATION INDUCED MUTANTS</subject><subject>RESOLUTION</subject><subject>Telomere</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1rGzEQhkVpSJ00194KKpTe1h1J-yEdjUmbgkMhbc5ClmazKl7JleSE_Pvuxia3nuYwz_sy8xDygcGSAbRfHzDEJVOqWTLO5RuyYCBVJdu6fUsWIKWsuqYW78hFzn8AQAnJz8m5bMQUlgtyt6J3xnlTfAz05nmbvKO3Zk-ffBloC3QTrafr-IjJhwdaBqTXofiE9NcQU6GrNNLY0_WQ4hhzHJEy_p6c9WaX8eo0L8n9t-vf65tq8_P7j_VqU1mh6lIp2YNjwrEOsO-3vGWANTdScKgFMtWBVa7vDG-dYa5W0HTcMlYDyka1aMUl-XTsjbl4na0vaAcbQ0BbdC0EV3JivhyZfYp_D5iLHn22uNuZgPGQddd1wGTdTODyCNoUc07Y633yo0nPmoGeRetZtJ5F61n0FPh4aj5sR3Sv-MnstP982ptsza5PJlifXzHRNkq81MgjhpOpR49pfgSDRTdJnv5w0f_vgn8z2ZYZ</recordid><startdate>19950901</startdate><enddate>19950901</enddate><creator>Raeymaekers, Peter</creator><creator>Van Zand, Karel</creator><creator>Jun, Lin</creator><creator>Höglund, Mattias</creator><creator>Cassiman, Jean-Jacques</creator><creator>Van den Berghe, Herman</creator><creator>Marynen, Peter</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19950901</creationdate><title>A Radiation Hybrid Map with 60 Loci Covering the Entire Short Arm of Chromosome 12</title><author>Raeymaekers, Peter ; Van Zand, Karel ; Jun, Lin ; Höglund, Mattias ; Cassiman, Jean-Jacques ; Van den Berghe, Herman ; Marynen, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-98f0d13d170effb2610e42a832043e1970c9df7a26da1d490572c1140e8596ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL MARKERS</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Cell Line</topic><topic>Centromere</topic><topic>CHROMOSOMAL ABERRATIONS</topic><topic>Chromosome Aberrations</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Artificial, Yeast</topic><topic>Chromosomes, Human, Pair 12 - radiation effects</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>CONTIGS</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>DNA SEQUENCING</topic><topic>ETIOLOGY</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE MUTATIONS</topic><topic>GENES</topic><topic>GENETIC MAPPING</topic><topic>Genetic Markers</topic><topic>GENETIC RADIATION EFFECTS</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Human</topic><topic>HUMAN CHROMOSOME 12</topic><topic>Humans</topic><topic>Hybrid Cells - radiation effects</topic><topic>Hypoxanthine Phosphoribosyltransferase - deficiency</topic><topic>Hypoxanthine Phosphoribosyltransferase - genetics</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Molecular Sequence Data</topic><topic>NEOPLASMS</topic><topic>Neoplasms - genetics</topic><topic>Oligonucleotide Probes</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>Polymorphism, Genetic</topic><topic>RADIATION INDUCED MUTANTS</topic><topic>RESOLUTION</topic><topic>Telomere</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raeymaekers, Peter</creatorcontrib><creatorcontrib>Van Zand, Karel</creatorcontrib><creatorcontrib>Jun, Lin</creatorcontrib><creatorcontrib>Höglund, Mattias</creatorcontrib><creatorcontrib>Cassiman, Jean-Jacques</creatorcontrib><creatorcontrib>Van den Berghe, Herman</creatorcontrib><creatorcontrib>Marynen, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raeymaekers, Peter</au><au>Van Zand, Karel</au><au>Jun, Lin</au><au>Höglund, Mattias</au><au>Cassiman, Jean-Jacques</au><au>Van den Berghe, Herman</au><au>Marynen, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Radiation Hybrid Map with 60 Loci Covering the Entire Short Arm of Chromosome 12</atitle><jtitle>Genomics</jtitle><addtitle>Genomics</addtitle><date>1995-09-01</date><risdate>1995</risdate><volume>29</volume><issue>1</issue><spage>170</spage><epage>178</epage><pages>170-178</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>We present a high-resolution radiation hybrid map of the short arm of human chromosome 12 containing 60 loci, including 44 STSs within or closely associated with expressed sequences, 11 highly polymorphic markers, 2 anonymous sequences, 2 subtelomeric sequences, and 1 centromeric sequence. The 60 loci fell into 48 unique retention patterns, providing a comprehensive map covering the entire short arm of chromosome 12 with an average resolution of approximately 800 kb. Twenty-two unique positions were ordered in a 1000:1 framework map with an average resolution of 1.8 Mb. The proposed order is in good agreement with recently published genetic maps, high-resolution FISH maps, and YAC contigs. The noted inconsistencies involved neighboring loci permutations. Our observations further suggest the existence of chromosomal "hot spots" for breakage during irradiation. In three regions an usually high number of breaks was noted between neighboring loci compared to the physical distance derived from existing YAC contigs. Some of these hot spots seem to coincide with known chromosomal aberrations, of which at least two have been involved in the etiology of cancer.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8530068</pmid><doi>10.1006/geno.1995.1228</doi><tpages>9</tpages></addata></record> |
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| ispartof | Genomics, 1995-09, Vol.29 (1), p.170-178 |
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| subjects | Animals Base Sequence Biological and medical sciences BIOLOGICAL MARKERS BIOLOGY AND MEDICINE, BASIC STUDIES Cell Line Centromere CHROMOSOMAL ABERRATIONS Chromosome Aberrations Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 12 - radiation effects Classical genetics, quantitative genetics, hybrids CONTIGS Cricetinae Cricetulus DNA SEQUENCING ETIOLOGY Fundamental and applied biological sciences. Psychology GENE MUTATIONS GENES GENETIC MAPPING Genetic Markers GENETIC RADIATION EFFECTS Genetics of eukaryotes. Biological and molecular evolution Human HUMAN CHROMOSOME 12 Humans Hybrid Cells - radiation effects Hypoxanthine Phosphoribosyltransferase - deficiency Hypoxanthine Phosphoribosyltransferase - genetics In Situ Hybridization, Fluorescence Molecular Sequence Data NEOPLASMS Neoplasms - genetics Oligonucleotide Probes POLYMERASE CHAIN REACTION Polymorphism, Genetic RADIATION INDUCED MUTANTS RESOLUTION Telomere |
| title | A Radiation Hybrid Map with 60 Loci Covering the Entire Short Arm of Chromosome 12 |
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