Characterization of the Hemagglutinin of Staphylococcus epidermidis
Adherence to biomaterials and production of biofilmis thought to be pivotal in the pathogenesis of prosthetic device infection by Staphylococcus epidermidis. In this study a strong association (P < .001)of hemagglutination with adherence and biofilm production was observed. Hemagglutination was n...
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| Veröffentlicht in: | The Journal of infectious diseases 1995-12, Vol.172 (6), p.1509-1518 |
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| container_title | The Journal of infectious diseases |
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| creator | Rupp, M. E. Sloot, N. Meyer, H.-G. W. Han, J. Gatermann, S. |
| description | Adherence to biomaterials and production of biofilmis thought to be pivotal in the pathogenesis of prosthetic device infection by Staphylococcus epidermidis. In this study a strong association (P < .001)of hemagglutination with adherence and biofilm production was observed. Hemagglutination was not associated with cell surface hydrophobicity (P = .906).Hemagglutination inhibition studies revealed that hemagglutination was not affected by heat, pH, cation concentration, proteolytic enzymes, biologic detergent, serum proteins, or subinhibitory antibiotics. Hemagglutination was abolished by periodate oxidation and digestion with glycosidases. It was markedly inhibited by β-lactose and its monosaccharide constituents in a concentration-dependent fashion. Hemagglutinin expression depended on the presence of glucose. Chemical analysis of a partially purified hemagglutinin preparation and cell-free hemagglutinating supernatants revealed little or no protein and small quantities of reducing sugars, pentose, ketose, hexosamine, uronic acid, and phosphate. Hemagglutinin of S. epidermidis appears to be a polysaccharide distinct from other known adhesins of S. epidermidis. |
| doi_str_mv | 10.1093/infdis/172.6.1509 |
| format | Article |
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E. ; Sloot, N. ; Meyer, H.-G. W. ; Han, J. ; Gatermann, S.</creator><creatorcontrib>Rupp, M. E. ; Sloot, N. ; Meyer, H.-G. W. ; Han, J. ; Gatermann, S.</creatorcontrib><description>Adherence to biomaterials and production of biofilmis thought to be pivotal in the pathogenesis of prosthetic device infection by Staphylococcus epidermidis. In this study a strong association (P < .001)of hemagglutination with adherence and biofilm production was observed. Hemagglutination was not associated with cell surface hydrophobicity (P = .906).Hemagglutination inhibition studies revealed that hemagglutination was not affected by heat, pH, cation concentration, proteolytic enzymes, biologic detergent, serum proteins, or subinhibitory antibiotics. Hemagglutination was abolished by periodate oxidation and digestion with glycosidases. It was markedly inhibited by β-lactose and its monosaccharide constituents in a concentration-dependent fashion. Hemagglutinin expression depended on the presence of glucose. Chemical analysis of a partially purified hemagglutinin preparation and cell-free hemagglutinating supernatants revealed little or no protein and small quantities of reducing sugars, pentose, ketose, hexosamine, uronic acid, and phosphate. Hemagglutinin of S. epidermidis appears to be a polysaccharide distinct from other known adhesins of S. epidermidis.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/172.6.1509</identifier><identifier>PMID: 7594710</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Bacteria ; Bacterial Adhesion ; Bacteriology ; Biofilms ; Biological and medical sciences ; Chemical suspensions ; Enzymes ; Erythrocytes ; Fundamental and applied biological sciences. Psychology ; Hemagglutination ; Hemagglutinins - analysis ; Hydrophobicity ; Infections ; Major Articles ; Microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Staphylococcus ; Staphylococcus epidermidis ; Staphylococcus epidermidis - chemistry ; Staphylococcus epidermidis - physiology ; Ungulates</subject><ispartof>The Journal of infectious diseases, 1995-12, Vol.172 (6), p.1509-1518</ispartof><rights>Copyright 1995 The University of Chicago</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-6ae9a3e3b8cce39d37e59f6e141bd39674d14b74d25704267c9822c537af59483</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30134662$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30134662$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2909008$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7594710$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rupp, M. E.</creatorcontrib><creatorcontrib>Sloot, N.</creatorcontrib><creatorcontrib>Meyer, H.-G. W.</creatorcontrib><creatorcontrib>Han, J.</creatorcontrib><creatorcontrib>Gatermann, S.</creatorcontrib><title>Characterization of the Hemagglutinin of Staphylococcus epidermidis</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Adherence to biomaterials and production of biofilmis thought to be pivotal in the pathogenesis of prosthetic device infection by Staphylococcus epidermidis. In this study a strong association (P < .001)of hemagglutination with adherence and biofilm production was observed. Hemagglutination was not associated with cell surface hydrophobicity (P = .906).Hemagglutination inhibition studies revealed that hemagglutination was not affected by heat, pH, cation concentration, proteolytic enzymes, biologic detergent, serum proteins, or subinhibitory antibiotics. Hemagglutination was abolished by periodate oxidation and digestion with glycosidases. It was markedly inhibited by β-lactose and its monosaccharide constituents in a concentration-dependent fashion. Hemagglutinin expression depended on the presence of glucose. Chemical analysis of a partially purified hemagglutinin preparation and cell-free hemagglutinating supernatants revealed little or no protein and small quantities of reducing sugars, pentose, ketose, hexosamine, uronic acid, and phosphate. Hemagglutinin of S. epidermidis appears to be a polysaccharide distinct from other known adhesins of S. epidermidis.</description><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Bacteria</subject><subject>Bacterial Adhesion</subject><subject>Bacteriology</subject><subject>Biofilms</subject><subject>Biological and medical sciences</subject><subject>Chemical suspensions</subject><subject>Enzymes</subject><subject>Erythrocytes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemagglutination</subject><subject>Hemagglutinins - analysis</subject><subject>Hydrophobicity</subject><subject>Infections</subject><subject>Major Articles</subject><subject>Microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Staphylococcus</subject><subject>Staphylococcus epidermidis</subject><subject>Staphylococcus epidermidis - chemistry</subject><subject>Staphylococcus epidermidis - physiology</subject><subject>Ungulates</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1uEzEUha0KVELpA3RRKQvEbtJre8Z3vKwiIEiVkCgtiI3leO40LvOT2h6J8vS4JKRLNrbs75xj38PYGYcFBy0v_NA2Pl5wFAu14BXoIzbjlcRCKS5fsBmAEAWvtX7FXsd4DwClVHjMjrHSJXKYseVyY4N1iYL_bZMfh_nYztOG5ivq7d1dNyU_-L-X18luN4_d6EbnpjinrW8o9D6__4a9bG0X6XS_n7CbD--_LlfF1eePn5aXV4WTZZ0KZUlbSXJdO0dSNxKp0q0iXvJ1I7XCsuHlOq-iQiiFQqdrIVwex7b5u7U8Ye92udswPkwUk-l9dNR1dqBxigYR88AA_xVyBI66xizkO6ELY4yBWrMNvrfh0XAwTw2bXcPZIYwyTw1nz_k-fFr31Bwc-0ozf7vnNjrbtcEOLif8kwkNGqB-jrmPaQwHLIHLUimRebHjPib6deA2_DQKJVZm9f2HWX3B229wnQ_yD5CKnXg</recordid><startdate>19951201</startdate><enddate>19951201</enddate><creator>Rupp, M. E.</creator><creator>Sloot, N.</creator><creator>Meyer, H.-G. W.</creator><creator>Han, J.</creator><creator>Gatermann, S.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19951201</creationdate><title>Characterization of the Hemagglutinin of Staphylococcus epidermidis</title><author>Rupp, M. E. ; Sloot, N. ; Meyer, H.-G. W. ; Han, J. ; Gatermann, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-6ae9a3e3b8cce39d37e59f6e141bd39674d14b74d25704267c9822c537af59483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Bacteria</topic><topic>Bacterial Adhesion</topic><topic>Bacteriology</topic><topic>Biofilms</topic><topic>Biological and medical sciences</topic><topic>Chemical suspensions</topic><topic>Enzymes</topic><topic>Erythrocytes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemagglutination</topic><topic>Hemagglutinins - analysis</topic><topic>Hydrophobicity</topic><topic>Infections</topic><topic>Major Articles</topic><topic>Microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Staphylococcus</topic><topic>Staphylococcus epidermidis</topic><topic>Staphylococcus epidermidis - chemistry</topic><topic>Staphylococcus epidermidis - physiology</topic><topic>Ungulates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rupp, M. E.</creatorcontrib><creatorcontrib>Sloot, N.</creatorcontrib><creatorcontrib>Meyer, H.-G. W.</creatorcontrib><creatorcontrib>Han, J.</creatorcontrib><creatorcontrib>Gatermann, S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rupp, M. E.</au><au>Sloot, N.</au><au>Meyer, H.-G. W.</au><au>Han, J.</au><au>Gatermann, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the Hemagglutinin of Staphylococcus epidermidis</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1995-12-01</date><risdate>1995</risdate><volume>172</volume><issue>6</issue><spage>1509</spage><epage>1518</epage><pages>1509-1518</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Adherence to biomaterials and production of biofilmis thought to be pivotal in the pathogenesis of prosthetic device infection by Staphylococcus epidermidis. In this study a strong association (P < .001)of hemagglutination with adherence and biofilm production was observed. Hemagglutination was not associated with cell surface hydrophobicity (P = .906).Hemagglutination inhibition studies revealed that hemagglutination was not affected by heat, pH, cation concentration, proteolytic enzymes, biologic detergent, serum proteins, or subinhibitory antibiotics. Hemagglutination was abolished by periodate oxidation and digestion with glycosidases. It was markedly inhibited by β-lactose and its monosaccharide constituents in a concentration-dependent fashion. Hemagglutinin expression depended on the presence of glucose. Chemical analysis of a partially purified hemagglutinin preparation and cell-free hemagglutinating supernatants revealed little or no protein and small quantities of reducing sugars, pentose, ketose, hexosamine, uronic acid, and phosphate. Hemagglutinin of S. epidermidis appears to be a polysaccharide distinct from other known adhesins of S. epidermidis.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>7594710</pmid><doi>10.1093/infdis/172.6.1509</doi><tpages>10</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 1995-12, Vol.172 (6), p.1509-1518 |
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| subjects | Anti-Inflammatory Agents, Non-Steroidal - pharmacology Bacteria Bacterial Adhesion Bacteriology Biofilms Biological and medical sciences Chemical suspensions Enzymes Erythrocytes Fundamental and applied biological sciences. Psychology Hemagglutination Hemagglutinins - analysis Hydrophobicity Infections Major Articles Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Staphylococcus Staphylococcus epidermidis Staphylococcus epidermidis - chemistry Staphylococcus epidermidis - physiology Ungulates |
| title | Characterization of the Hemagglutinin of Staphylococcus epidermidis |
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