Immunocytochemical localization of motilin-containing cells in the rabbit gastrointestinal tract
Motilin-immunopositive cells (Mo cells) are known to be present in the upper small intestine of various species, including man. However, whether Mo cells are present in the rabbit gastrointestinal tract remained to be elucidated. Therefore, this study was designed to investigate the distribution of...
Gespeichert in:
| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 1995, Vol.16 (5), p.883-887 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 887 |
|---|---|
| container_issue | 5 |
| container_start_page | 883 |
| container_title | Peptides (New York, N.Y. : 1980) |
| container_volume | 16 |
| creator | Satoh, Minoru Sakai, Takafumi Koyama, Haruko Shiba, Yoshihiro Itoh, Zen |
| description | Motilin-immunopositive cells (Mo cells) are known to be present in the upper small intestine of various species, including man. However, whether Mo cells are present in the rabbit gastrointestinal tract remained to be elucidated. Therefore, this study was designed to investigate the distribution of Mo cells in the rabbit gastrointestinal tract by the avidin-biotin-peroxidase complex method using a new anti-motilin serum (CPV2) raised in chickens. The results of an enzyme-linked immunosorbent assay suggested that this antiserum recognized the
C-terminal region of the motilin molecule. Motilin-immunopositive cells were found in the epithelia of the crypts and villi throughout the rabbit gastrointestinal tract from the gastric antrum to the distal colon, but no immunostaining occurred in the gastric body. Morphometric analysis revealed that Mo cells were localized preferentially in the upper small intestine, as reported for other species, and the cell densities (cells/mm
2, mean ± SE) were: gastric antrum (0.41 ± 0.16), duodenum (8.2 ± 0.8), jejunum (1.9 ± 0.5), ileum (0.62 ± 0.14), cecum (0.19 ± 0.05), proximal colon (0.13 ± 0.03), and distal colon (0.39 ± 0.18). Our results demonstrated conclusively that Mo cells exist in the rabbit gastrointestinal tract and showed for the first time their regional distribution. Furthermore, our new chicken antiserum would appear to be a useful tool for the determination of plasma motilin concentrations by radioimmunoassay and for the immunoneutralization of endogenous motilin in the rabbit. |
| doi_str_mv | 10.1016/0196-9781(95)00046-M |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77699388</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>019697819500046M</els_id><sourcerecordid>77699388</sourcerecordid><originalsourceid>FETCH-LOGICAL-c423t-fbe389e9ba6ba499dfa3907f8bca11f9df41da9b5ddc154d5256fc8bfab92dd3</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMotVb_gcKeRA-rSfczF0GKH4UWL73HfLYju4kmWaH-endt6dHLDMO8887Mg9AlwXcEk_IeE1qmtKrJDS1uMcZ5mS6P0JjUVZYWpKTHaHyQnKKzED4GUU7rERpVeUWzDI_R-7xtO-vkNjq50S1I3iSN6yP88AjOJs4krYvQgE2ls5GDBbtOpG6akIBN4kYnngsBMVnzEL0DG3WIYHuf6LmM5-jE8Cboi32eoNXz02r2mi7eXuazx0Uq82kWUyN0VlNNBS8FzylVhmcUV6YWkhNi-jonilNRKCVJkatiWpRG1sJwQadKZRN0vbP99O6r6y9gLYThSm616wKrqpLSrK57Yb4TSu9C8NqwTw8t91tGMBu4sgEaG6AxWrA_rmzZj13t_TvRanUY2oPs-w-7vu5__AbtWZCgrdQKvJaRKQf_L_gFeaKLfQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77699388</pqid></control><display><type>article</type><title>Immunocytochemical localization of motilin-containing cells in the rabbit gastrointestinal tract</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Satoh, Minoru ; Sakai, Takafumi ; Koyama, Haruko ; Shiba, Yoshihiro ; Itoh, Zen</creator><creatorcontrib>Satoh, Minoru ; Sakai, Takafumi ; Koyama, Haruko ; Shiba, Yoshihiro ; Itoh, Zen</creatorcontrib><description>Motilin-immunopositive cells (Mo cells) are known to be present in the upper small intestine of various species, including man. However, whether Mo cells are present in the rabbit gastrointestinal tract remained to be elucidated. Therefore, this study was designed to investigate the distribution of Mo cells in the rabbit gastrointestinal tract by the avidin-biotin-peroxidase complex method using a new anti-motilin serum (CPV2) raised in chickens. The results of an enzyme-linked immunosorbent assay suggested that this antiserum recognized the
C-terminal region of the motilin molecule. Motilin-immunopositive cells were found in the epithelia of the crypts and villi throughout the rabbit gastrointestinal tract from the gastric antrum to the distal colon, but no immunostaining occurred in the gastric body. Morphometric analysis revealed that Mo cells were localized preferentially in the upper small intestine, as reported for other species, and the cell densities (cells/mm
2, mean ± SE) were: gastric antrum (0.41 ± 0.16), duodenum (8.2 ± 0.8), jejunum (1.9 ± 0.5), ileum (0.62 ± 0.14), cecum (0.19 ± 0.05), proximal colon (0.13 ± 0.03), and distal colon (0.39 ± 0.18). Our results demonstrated conclusively that Mo cells exist in the rabbit gastrointestinal tract and showed for the first time their regional distribution. Furthermore, our new chicken antiserum would appear to be a useful tool for the determination of plasma motilin concentrations by radioimmunoassay and for the immunoneutralization of endogenous motilin in the rabbit.</description><identifier>ISSN: 0196-9781</identifier><identifier>EISSN: 1873-5169</identifier><identifier>DOI: 10.1016/0196-9781(95)00046-M</identifier><identifier>PMID: 7479330</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Chickens - immunology ; Colon - cytology ; Digestive System - cytology ; Dogs ; Enzyme-Linked Immunosorbent Assay - methods ; Epithelial Cells ; Female ; Gastric Mucosa - cytology ; Gastrointestinal tract ; Humans ; Immunocytochemistry ; Immunohistochemistry - methods ; Intestinal Mucosa - cytology ; Intestine, Small - cytology ; Motilin ; Motilin - analysis ; Motilin - blood ; Pyloric Antrum - cytology ; Rabbit ; Rabbits ; Swine</subject><ispartof>Peptides (New York, N.Y. : 1980), 1995, Vol.16 (5), p.883-887</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-fbe389e9ba6ba499dfa3907f8bca11f9df41da9b5ddc154d5256fc8bfab92dd3</citedby><cites>FETCH-LOGICAL-c423t-fbe389e9ba6ba499dfa3907f8bca11f9df41da9b5ddc154d5256fc8bfab92dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/019697819500046M$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7479330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Satoh, Minoru</creatorcontrib><creatorcontrib>Sakai, Takafumi</creatorcontrib><creatorcontrib>Koyama, Haruko</creatorcontrib><creatorcontrib>Shiba, Yoshihiro</creatorcontrib><creatorcontrib>Itoh, Zen</creatorcontrib><title>Immunocytochemical localization of motilin-containing cells in the rabbit gastrointestinal tract</title><title>Peptides (New York, N.Y. : 1980)</title><addtitle>Peptides</addtitle><description>Motilin-immunopositive cells (Mo cells) are known to be present in the upper small intestine of various species, including man. However, whether Mo cells are present in the rabbit gastrointestinal tract remained to be elucidated. Therefore, this study was designed to investigate the distribution of Mo cells in the rabbit gastrointestinal tract by the avidin-biotin-peroxidase complex method using a new anti-motilin serum (CPV2) raised in chickens. The results of an enzyme-linked immunosorbent assay suggested that this antiserum recognized the
C-terminal region of the motilin molecule. Motilin-immunopositive cells were found in the epithelia of the crypts and villi throughout the rabbit gastrointestinal tract from the gastric antrum to the distal colon, but no immunostaining occurred in the gastric body. Morphometric analysis revealed that Mo cells were localized preferentially in the upper small intestine, as reported for other species, and the cell densities (cells/mm
2, mean ± SE) were: gastric antrum (0.41 ± 0.16), duodenum (8.2 ± 0.8), jejunum (1.9 ± 0.5), ileum (0.62 ± 0.14), cecum (0.19 ± 0.05), proximal colon (0.13 ± 0.03), and distal colon (0.39 ± 0.18). Our results demonstrated conclusively that Mo cells exist in the rabbit gastrointestinal tract and showed for the first time their regional distribution. Furthermore, our new chicken antiserum would appear to be a useful tool for the determination of plasma motilin concentrations by radioimmunoassay and for the immunoneutralization of endogenous motilin in the rabbit.</description><subject>Animals</subject><subject>Chickens - immunology</subject><subject>Colon - cytology</subject><subject>Digestive System - cytology</subject><subject>Dogs</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Epithelial Cells</subject><subject>Female</subject><subject>Gastric Mucosa - cytology</subject><subject>Gastrointestinal tract</subject><subject>Humans</subject><subject>Immunocytochemistry</subject><subject>Immunohistochemistry - methods</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestine, Small - cytology</subject><subject>Motilin</subject><subject>Motilin - analysis</subject><subject>Motilin - blood</subject><subject>Pyloric Antrum - cytology</subject><subject>Rabbit</subject><subject>Rabbits</subject><subject>Swine</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcKeRA-rSfczF0GKH4UWL73HfLYju4kmWaH-endt6dHLDMO8887Mg9AlwXcEk_IeE1qmtKrJDS1uMcZ5mS6P0JjUVZYWpKTHaHyQnKKzED4GUU7rERpVeUWzDI_R-7xtO-vkNjq50S1I3iSN6yP88AjOJs4krYvQgE2ls5GDBbtOpG6akIBN4kYnngsBMVnzEL0DG3WIYHuf6LmM5-jE8Cboi32eoNXz02r2mi7eXuazx0Uq82kWUyN0VlNNBS8FzylVhmcUV6YWkhNi-jonilNRKCVJkatiWpRG1sJwQadKZRN0vbP99O6r6y9gLYThSm616wKrqpLSrK57Yb4TSu9C8NqwTw8t91tGMBu4sgEaG6AxWrA_rmzZj13t_TvRanUY2oPs-w-7vu5__AbtWZCgrdQKvJaRKQf_L_gFeaKLfQ</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Satoh, Minoru</creator><creator>Sakai, Takafumi</creator><creator>Koyama, Haruko</creator><creator>Shiba, Yoshihiro</creator><creator>Itoh, Zen</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Immunocytochemical localization of motilin-containing cells in the rabbit gastrointestinal tract</title><author>Satoh, Minoru ; Sakai, Takafumi ; Koyama, Haruko ; Shiba, Yoshihiro ; Itoh, Zen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-fbe389e9ba6ba499dfa3907f8bca11f9df41da9b5ddc154d5256fc8bfab92dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Chickens - immunology</topic><topic>Colon - cytology</topic><topic>Digestive System - cytology</topic><topic>Dogs</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Epithelial Cells</topic><topic>Female</topic><topic>Gastric Mucosa - cytology</topic><topic>Gastrointestinal tract</topic><topic>Humans</topic><topic>Immunocytochemistry</topic><topic>Immunohistochemistry - methods</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestine, Small - cytology</topic><topic>Motilin</topic><topic>Motilin - analysis</topic><topic>Motilin - blood</topic><topic>Pyloric Antrum - cytology</topic><topic>Rabbit</topic><topic>Rabbits</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Satoh, Minoru</creatorcontrib><creatorcontrib>Sakai, Takafumi</creatorcontrib><creatorcontrib>Koyama, Haruko</creatorcontrib><creatorcontrib>Shiba, Yoshihiro</creatorcontrib><creatorcontrib>Itoh, Zen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Satoh, Minoru</au><au>Sakai, Takafumi</au><au>Koyama, Haruko</au><au>Shiba, Yoshihiro</au><au>Itoh, Zen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunocytochemical localization of motilin-containing cells in the rabbit gastrointestinal tract</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>1995</date><risdate>1995</risdate><volume>16</volume><issue>5</issue><spage>883</spage><epage>887</epage><pages>883-887</pages><issn>0196-9781</issn><eissn>1873-5169</eissn><abstract>Motilin-immunopositive cells (Mo cells) are known to be present in the upper small intestine of various species, including man. However, whether Mo cells are present in the rabbit gastrointestinal tract remained to be elucidated. Therefore, this study was designed to investigate the distribution of Mo cells in the rabbit gastrointestinal tract by the avidin-biotin-peroxidase complex method using a new anti-motilin serum (CPV2) raised in chickens. The results of an enzyme-linked immunosorbent assay suggested that this antiserum recognized the
C-terminal region of the motilin molecule. Motilin-immunopositive cells were found in the epithelia of the crypts and villi throughout the rabbit gastrointestinal tract from the gastric antrum to the distal colon, but no immunostaining occurred in the gastric body. Morphometric analysis revealed that Mo cells were localized preferentially in the upper small intestine, as reported for other species, and the cell densities (cells/mm
2, mean ± SE) were: gastric antrum (0.41 ± 0.16), duodenum (8.2 ± 0.8), jejunum (1.9 ± 0.5), ileum (0.62 ± 0.14), cecum (0.19 ± 0.05), proximal colon (0.13 ± 0.03), and distal colon (0.39 ± 0.18). Our results demonstrated conclusively that Mo cells exist in the rabbit gastrointestinal tract and showed for the first time their regional distribution. Furthermore, our new chicken antiserum would appear to be a useful tool for the determination of plasma motilin concentrations by radioimmunoassay and for the immunoneutralization of endogenous motilin in the rabbit.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7479330</pmid><doi>10.1016/0196-9781(95)00046-M</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0196-9781 |
| ispartof | Peptides (New York, N.Y. : 1980), 1995, Vol.16 (5), p.883-887 |
| issn | 0196-9781 1873-5169 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77699388 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Chickens - immunology Colon - cytology Digestive System - cytology Dogs Enzyme-Linked Immunosorbent Assay - methods Epithelial Cells Female Gastric Mucosa - cytology Gastrointestinal tract Humans Immunocytochemistry Immunohistochemistry - methods Intestinal Mucosa - cytology Intestine, Small - cytology Motilin Motilin - analysis Motilin - blood Pyloric Antrum - cytology Rabbit Rabbits Swine |
| title | Immunocytochemical localization of motilin-containing cells in the rabbit gastrointestinal tract |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T13%3A53%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunocytochemical%20localization%20of%20motilin-containing%20cells%20in%20the%20rabbit%20gastrointestinal%20tract&rft.jtitle=Peptides%20(New%20York,%20N.Y.%20:%201980)&rft.au=Satoh,%20Minoru&rft.date=1995&rft.volume=16&rft.issue=5&rft.spage=883&rft.epage=887&rft.pages=883-887&rft.issn=0196-9781&rft.eissn=1873-5169&rft_id=info:doi/10.1016/0196-9781(95)00046-M&rft_dat=%3Cproquest_cross%3E77699388%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77699388&rft_id=info:pmid/7479330&rft_els_id=019697819500046M&rfr_iscdi=true |