Drug resistance and Acanthamoeba Keratitis: The quest for alternative antiprotozoal chemotherapy
Trophozoites and cysts of 20 isolates of Acanthamoeba from the cornea and five from related samples were tested in vitro for sensitivity to ten drugs (three aromatic dia-midines, two aminoglycosides, two macrolides, a polyene macrolide antibiotic, an organoarsenical and an antimetabolite) and two ca...
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| Veröffentlicht in: | Eye (London) 1994-01, Vol.8 (5), p.555-563 |
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| creator | Hay, John Kirkness, Colin M Seal, David V Wright, Peter |
| description | Trophozoites and cysts of 20 isolates of
Acanthamoeba
from the cornea and five from related samples were tested
in vitro
for sensitivity to ten drugs (three aromatic dia-midines, two aminoglycosides, two macrolides, a polyene macrolide antibiotic, an organoarsenical and an antimetabolite) and two cationic antiseptics (chlorhexidine and polyhexamethylene biguanide, PHMB). Only chlorhexidine and PHMB showed uniform amoebacidal activity. Aromatic diamidines (pentamidine isethionate, propamidine isethionate and diminazene aceturate) generally proved effective against both forms of the amoeba; only pentamidine gave synergy with the biguanide while propamidine gave an additive effect. Other drugs tested proved erratic or ineffective against different isolates. Chlorhexidine alone, or together with propamidine, was subsequently used in two patients with proven
Acanthamoeba
keratitis; the causative isolates were sensitive to the individual compounds and to the combination
in vitro
. The treatment provided resolution of the clinical disease; amoebae were shown to be non-viable by histology and culture. The combination of chlorhexidine and propamidine is recommended for treatment of proven
Acanthamoeba
keratitis. |
| doi_str_mv | 10.1038/eye.1994.137 |
| format | Article |
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Acanthamoeba
from the cornea and five from related samples were tested
in vitro
for sensitivity to ten drugs (three aromatic dia-midines, two aminoglycosides, two macrolides, a polyene macrolide antibiotic, an organoarsenical and an antimetabolite) and two cationic antiseptics (chlorhexidine and polyhexamethylene biguanide, PHMB). Only chlorhexidine and PHMB showed uniform amoebacidal activity. Aromatic diamidines (pentamidine isethionate, propamidine isethionate and diminazene aceturate) generally proved effective against both forms of the amoeba; only pentamidine gave synergy with the biguanide while propamidine gave an additive effect. Other drugs tested proved erratic or ineffective against different isolates. Chlorhexidine alone, or together with propamidine, was subsequently used in two patients with proven
Acanthamoeba
keratitis; the causative isolates were sensitive to the individual compounds and to the combination
in vitro
. The treatment provided resolution of the clinical disease; amoebae were shown to be non-viable by histology and culture. The combination of chlorhexidine and propamidine is recommended for treatment of proven
Acanthamoeba
keratitis.</description><identifier>ISSN: 0950-222X</identifier><identifier>EISSN: 1476-5454</identifier><identifier>DOI: 10.1038/eye.1994.137</identifier><identifier>PMID: 7835453</identifier><identifier>CODEN: EYEEEC</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Acanthamoeba - drug effects ; Acanthamoeba Keratitis - drug therapy ; Amebicides - therapeutic use ; Animals ; Benzamidines - therapeutic use ; Biological and medical sciences ; Chlorhexidine - therapeutic use ; Diseases of cornea, anterior segment and sclera ; Drug Resistance ; Drug Therapy, Combination ; Humans ; Laboratory Medicine ; Male ; Medical sciences ; Medicine ; Medicine & Public Health ; Middle Aged ; Ophthalmology ; Pharmaceutical Sciences/Technology ; Surgery ; Surgical Oncology</subject><ispartof>Eye (London), 1994-01, Vol.8 (5), p.555-563</ispartof><rights>Royal College of Ophthalmologists 1994</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-f5e7f0c39549b59065623bd8ed8b9967465104c20fb49bad7166e8535149dabc3</citedby><cites>FETCH-LOGICAL-c457t-f5e7f0c39549b59065623bd8ed8b9967465104c20fb49bad7166e8535149dabc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/eye.1994.137$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/eye.1994.137$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3347650$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7835453$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hay, John</creatorcontrib><creatorcontrib>Kirkness, Colin M</creatorcontrib><creatorcontrib>Seal, David V</creatorcontrib><creatorcontrib>Wright, Peter</creatorcontrib><title>Drug resistance and Acanthamoeba Keratitis: The quest for alternative antiprotozoal chemotherapy</title><title>Eye (London)</title><addtitle>Eye</addtitle><addtitle>Eye (Lond)</addtitle><description>Trophozoites and cysts of 20 isolates of
Acanthamoeba
from the cornea and five from related samples were tested
in vitro
for sensitivity to ten drugs (three aromatic dia-midines, two aminoglycosides, two macrolides, a polyene macrolide antibiotic, an organoarsenical and an antimetabolite) and two cationic antiseptics (chlorhexidine and polyhexamethylene biguanide, PHMB). Only chlorhexidine and PHMB showed uniform amoebacidal activity. Aromatic diamidines (pentamidine isethionate, propamidine isethionate and diminazene aceturate) generally proved effective against both forms of the amoeba; only pentamidine gave synergy with the biguanide while propamidine gave an additive effect. Other drugs tested proved erratic or ineffective against different isolates. Chlorhexidine alone, or together with propamidine, was subsequently used in two patients with proven
Acanthamoeba
keratitis; the causative isolates were sensitive to the individual compounds and to the combination
in vitro
. The treatment provided resolution of the clinical disease; amoebae were shown to be non-viable by histology and culture. The combination of chlorhexidine and propamidine is recommended for treatment of proven
Acanthamoeba
keratitis.</description><subject>Acanthamoeba - drug effects</subject><subject>Acanthamoeba Keratitis - drug therapy</subject><subject>Amebicides - therapeutic use</subject><subject>Animals</subject><subject>Benzamidines - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Chlorhexidine - therapeutic use</subject><subject>Diseases of cornea, anterior segment and sclera</subject><subject>Drug Resistance</subject><subject>Drug Therapy, Combination</subject><subject>Humans</subject><subject>Laboratory Medicine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Ophthalmology</subject><subject>Pharmaceutical Sciences/Technology</subject><subject>Surgery</subject><subject>Surgical Oncology</subject><issn>0950-222X</issn><issn>1476-5454</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU1P3DAQhq2qiC7QW6-VfKg4ka0d23HMDdHyoSJxoVJvruNM2KAkXmwHafvrO8uuOHHy4X3m9egZQr5wtuRM1N9hA0tujFxyoT-QBZe6KpRU8iNZMKNYUZbln0_kKKUnxjDU7JAc6logIhbk7484P9IIqU_ZTR6om1p64d2UV24M0Dj6C6LLfe7TOX1YAX2eIWXahUjdkCFOmL1sp3K_jiGHf8EN1K9gDHmFg-vNCTno3JDg8_49Jr-vfj5c3hR399e3lxd3hZdK56JToDvmhVHSNMqwSlWlaNoa2roxptKyUpxJX7KuQcC1mlcV1EooLk3rGi-OyemuF9d43dGOffIwDG6CMCerdWVqLELwbAf6GFKK0Nl17EcXN5YzuxVqUajdCrUoFPGv-965GaF9g_cGMf-2z13ybugiWuzTGyYEnkMxxIodljCZHiHapzCjvSG9_-1_WYyOKQ</recordid><startdate>19940101</startdate><enddate>19940101</enddate><creator>Hay, John</creator><creator>Kirkness, Colin M</creator><creator>Seal, David V</creator><creator>Wright, Peter</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940101</creationdate><title>Drug resistance and Acanthamoeba Keratitis: The quest for alternative antiprotozoal chemotherapy</title><author>Hay, John ; Kirkness, Colin M ; Seal, David V ; Wright, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-f5e7f0c39549b59065623bd8ed8b9967465104c20fb49bad7166e8535149dabc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Acanthamoeba - drug effects</topic><topic>Acanthamoeba Keratitis - drug therapy</topic><topic>Amebicides - therapeutic use</topic><topic>Animals</topic><topic>Benzamidines - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Chlorhexidine - therapeutic use</topic><topic>Diseases of cornea, anterior segment and sclera</topic><topic>Drug Resistance</topic><topic>Drug Therapy, Combination</topic><topic>Humans</topic><topic>Laboratory Medicine</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Ophthalmology</topic><topic>Pharmaceutical Sciences/Technology</topic><topic>Surgery</topic><topic>Surgical Oncology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hay, John</creatorcontrib><creatorcontrib>Kirkness, Colin M</creatorcontrib><creatorcontrib>Seal, David V</creatorcontrib><creatorcontrib>Wright, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Eye (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hay, John</au><au>Kirkness, Colin M</au><au>Seal, David V</au><au>Wright, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drug resistance and Acanthamoeba Keratitis: The quest for alternative antiprotozoal chemotherapy</atitle><jtitle>Eye (London)</jtitle><stitle>Eye</stitle><addtitle>Eye (Lond)</addtitle><date>1994-01-01</date><risdate>1994</risdate><volume>8</volume><issue>5</issue><spage>555</spage><epage>563</epage><pages>555-563</pages><issn>0950-222X</issn><eissn>1476-5454</eissn><coden>EYEEEC</coden><abstract>Trophozoites and cysts of 20 isolates of
Acanthamoeba
from the cornea and five from related samples were tested
in vitro
for sensitivity to ten drugs (three aromatic dia-midines, two aminoglycosides, two macrolides, a polyene macrolide antibiotic, an organoarsenical and an antimetabolite) and two cationic antiseptics (chlorhexidine and polyhexamethylene biguanide, PHMB). Only chlorhexidine and PHMB showed uniform amoebacidal activity. Aromatic diamidines (pentamidine isethionate, propamidine isethionate and diminazene aceturate) generally proved effective against both forms of the amoeba; only pentamidine gave synergy with the biguanide while propamidine gave an additive effect. Other drugs tested proved erratic or ineffective against different isolates. Chlorhexidine alone, or together with propamidine, was subsequently used in two patients with proven
Acanthamoeba
keratitis; the causative isolates were sensitive to the individual compounds and to the combination
in vitro
. The treatment provided resolution of the clinical disease; amoebae were shown to be non-viable by histology and culture. The combination of chlorhexidine and propamidine is recommended for treatment of proven
Acanthamoeba
keratitis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>7835453</pmid><doi>10.1038/eye.1994.137</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Eye (London), 1994-01, Vol.8 (5), p.555-563 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Acanthamoeba - drug effects Acanthamoeba Keratitis - drug therapy Amebicides - therapeutic use Animals Benzamidines - therapeutic use Biological and medical sciences Chlorhexidine - therapeutic use Diseases of cornea, anterior segment and sclera Drug Resistance Drug Therapy, Combination Humans Laboratory Medicine Male Medical sciences Medicine Medicine & Public Health Middle Aged Ophthalmology Pharmaceutical Sciences/Technology Surgery Surgical Oncology |
| title | Drug resistance and Acanthamoeba Keratitis: The quest for alternative antiprotozoal chemotherapy |
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