Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts
Occlusion of saphenous vein grafts is a major problem after coronary artery bypass grafting. Segments of occluded and suboccluded implanted aortocoronary grafts were obtained during re‐intervention bypass grafting in 47 patients yielding a total of 80 vein grafts. The grafts were studied by immunohi...
Gespeichert in:
Veröffentlicht in: | Histopathology 1994-10, Vol.25 (4), p.365-371 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 371 |
---|---|
container_issue | 4 |
container_start_page | 365 |
container_title | Histopathology |
container_volume | 25 |
creator | KOCKX, M.M. CAMBIER, B.A. BORTIER, H.E. MEYER, G.R. DE DECLERCQ, S.C. CAUWELAERT, P.A. VAN BULTINCK, J. |
description | Occlusion of saphenous vein grafts is a major problem after coronary artery bypass grafting. Segments of occluded and suboccluded implanted aortocoronary grafts were obtained during re‐intervention bypass grafting in 47 patients yielding a total of 80 vein grafts. The grafts were studied by immunohistochemistry for smooth muscle cells (ÉL‐SMC actin), macrophages (HAM56), cell replication (PCNA, Ki‐67) and transmission and scanning electronmicroscopy (TEM, SEM). In 81% of the examined grafts the (sub)occlusion was due to a myo‐intimal thickening and an associated luminal accumulation of foam cells and mural thrombi. The foam cells were constantly found at the luminal site of the myo‐intimal thickening and within the luminal part of adherent thrombi. Transmission electronmicroscopy demonstrated phagocytosis of platelets and platelet fragments by the foam cells. A significant fraction of the foam cells demonstrated nuclear immunoreactivity for Ki‐67 and PCNA. The myo‐intimal thickening of the vein grafts was composed of smooth muscle cells lying in a fibrous tissue matrix. The smooth muscle cells were surrounded by prominent basal lamina and showed ultrastructural features of apoptosis. Our results support the hypothesis that phagocytosis of lipid rich platelets by monocytes set up a mechanism for foam cell formation and replication in human saphenous vein grafts. The transformation of a smooth muscle cell rich myo‐intimal thickening towards a fibrous, cell poor intimal thickening could be induced by progressive smooth muscle cell loss through apoptosis. |
doi_str_mv | 10.1111/j.1365-2559.1994.tb01355.x |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77695468</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>77695468</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5025-33c278c11928495863fcf4b29d7cfbee534d37b87385c65445932f18877ea35b3</originalsourceid><addsrcrecordid>eNqVkFGL1DAUhYso67j6E4Qg4ltrkts0iQ-CLO7OyrIKrsxjSDOpk7FtatLq7L83pWXezUsg57vnnpwse0NwQdJ5fywIVCynjMmCSFkWY40JMFacnmSbs_Q022DAMsek4s-zFzEeMSYcKL3ILrgAJkq6yX5ce90hY9sWBTu0zujR-R7pfo9i5_14QN0UTWsXRA9-GH10EbkeHaZO9yjq4WB7P0X0x6bHn0E3Y3yZPWt0G-2r9b5Mez4_XG3zu683t1ef7nLDMGU5gKFcGEIkFaVkooLGNGVN5Z6bpraWQbkHXgsOgpmKlSWTQBsiBOdWA6vhMnu3-A7B_55sHFXn4pxU9zZFUpxXkpWVSOCHBTTBxxhso4bgOh0eFcFq7lQd1VycmotTc6dq7VSd0vDrdctUd3Z_Hl1LTPrbVdfR6LYJujcunjGA9L9KJuzjgv11rX38jwBqe_s9SckgXwxcHO3pbKDDL1Vx4Ezt7m_U9nr3bXe_fVBf4B9_gaKv</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77695468</pqid></control><display><type>article</type><title>Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts</title><source>Wiley-Blackwell Journals</source><source>MEDLINE</source><creator>KOCKX, M.M. ; CAMBIER, B.A. ; BORTIER, H.E. ; MEYER, G.R. DE ; DECLERCQ, S.C. ; CAUWELAERT, P.A. VAN ; BULTINCK, J.</creator><creatorcontrib>KOCKX, M.M. ; CAMBIER, B.A. ; BORTIER, H.E. ; MEYER, G.R. DE ; DECLERCQ, S.C. ; CAUWELAERT, P.A. VAN ; BULTINCK, J.</creatorcontrib><description>Occlusion of saphenous vein grafts is a major problem after coronary artery bypass grafting. Segments of occluded and suboccluded implanted aortocoronary grafts were obtained during re‐intervention bypass grafting in 47 patients yielding a total of 80 vein grafts. The grafts were studied by immunohistochemistry for smooth muscle cells (ÉL‐SMC actin), macrophages (HAM56), cell replication (PCNA, Ki‐67) and transmission and scanning electronmicroscopy (TEM, SEM). In 81% of the examined grafts the (sub)occlusion was due to a myo‐intimal thickening and an associated luminal accumulation of foam cells and mural thrombi. The foam cells were constantly found at the luminal site of the myo‐intimal thickening and within the luminal part of adherent thrombi. Transmission electronmicroscopy demonstrated phagocytosis of platelets and platelet fragments by the foam cells. A significant fraction of the foam cells demonstrated nuclear immunoreactivity for Ki‐67 and PCNA. The myo‐intimal thickening of the vein grafts was composed of smooth muscle cells lying in a fibrous tissue matrix. The smooth muscle cells were surrounded by prominent basal lamina and showed ultrastructural features of apoptosis. Our results support the hypothesis that phagocytosis of lipid rich platelets by monocytes set up a mechanism for foam cell formation and replication in human saphenous vein grafts. The transformation of a smooth muscle cell rich myo‐intimal thickening towards a fibrous, cell poor intimal thickening could be induced by progressive smooth muscle cell loss through apoptosis.</description><identifier>ISSN: 0309-0167</identifier><identifier>EISSN: 1365-2559</identifier><identifier>DOI: 10.1111/j.1365-2559.1994.tb01355.x</identifier><identifier>PMID: 7835842</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Apoptosis ; Biological and medical sciences ; Cell Division ; Coronary Artery Bypass ; Endothelium, Vascular - ultrastructure ; foam cells ; Foam Cells - chemistry ; Foam Cells - cytology ; Foam Cells - ultrastructure ; Graft Occlusion, Vascular - pathology ; Humans ; Immunohistochemistry ; Keywords: coronary artery bypass ; Keywords: coronary artery bypass, saphenous vein, foam cells, smooth muscle cells, apoptosis, thrombosis ; Ki-67 Antigen ; Medical sciences ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Middle Aged ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - ultrastructure ; Neoplasm Proteins - analysis ; Nuclear Proteins - analysis ; Proliferating Cell Nuclear Antigen - analysis ; saphenous vein ; Saphenous Vein - cytology ; Saphenous Vein - transplantation ; smooth muscle cells ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the heart ; thrombosis ; Thrombosis - pathology ; Transplantation, Autologous ; Tunica Intima - ultrastructure</subject><ispartof>Histopathology, 1994-10, Vol.25 (4), p.365-371</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5025-33c278c11928495863fcf4b29d7cfbee534d37b87385c65445932f18877ea35b3</citedby><cites>FETCH-LOGICAL-c5025-33c278c11928495863fcf4b29d7cfbee534d37b87385c65445932f18877ea35b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2559.1994.tb01355.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2559.1994.tb01355.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3319269$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7835842$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KOCKX, M.M.</creatorcontrib><creatorcontrib>CAMBIER, B.A.</creatorcontrib><creatorcontrib>BORTIER, H.E.</creatorcontrib><creatorcontrib>MEYER, G.R. DE</creatorcontrib><creatorcontrib>DECLERCQ, S.C.</creatorcontrib><creatorcontrib>CAUWELAERT, P.A. VAN</creatorcontrib><creatorcontrib>BULTINCK, J.</creatorcontrib><title>Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts</title><title>Histopathology</title><addtitle>Histopathology</addtitle><description>Occlusion of saphenous vein grafts is a major problem after coronary artery bypass grafting. Segments of occluded and suboccluded implanted aortocoronary grafts were obtained during re‐intervention bypass grafting in 47 patients yielding a total of 80 vein grafts. The grafts were studied by immunohistochemistry for smooth muscle cells (ÉL‐SMC actin), macrophages (HAM56), cell replication (PCNA, Ki‐67) and transmission and scanning electronmicroscopy (TEM, SEM). In 81% of the examined grafts the (sub)occlusion was due to a myo‐intimal thickening and an associated luminal accumulation of foam cells and mural thrombi. The foam cells were constantly found at the luminal site of the myo‐intimal thickening and within the luminal part of adherent thrombi. Transmission electronmicroscopy demonstrated phagocytosis of platelets and platelet fragments by the foam cells. A significant fraction of the foam cells demonstrated nuclear immunoreactivity for Ki‐67 and PCNA. The myo‐intimal thickening of the vein grafts was composed of smooth muscle cells lying in a fibrous tissue matrix. The smooth muscle cells were surrounded by prominent basal lamina and showed ultrastructural features of apoptosis. Our results support the hypothesis that phagocytosis of lipid rich platelets by monocytes set up a mechanism for foam cell formation and replication in human saphenous vein grafts. The transformation of a smooth muscle cell rich myo‐intimal thickening towards a fibrous, cell poor intimal thickening could be induced by progressive smooth muscle cell loss through apoptosis.</description><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Coronary Artery Bypass</subject><subject>Endothelium, Vascular - ultrastructure</subject><subject>foam cells</subject><subject>Foam Cells - chemistry</subject><subject>Foam Cells - cytology</subject><subject>Foam Cells - ultrastructure</subject><subject>Graft Occlusion, Vascular - pathology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Keywords: coronary artery bypass</subject><subject>Keywords: coronary artery bypass, saphenous vein, foam cells, smooth muscle cells, apoptosis, thrombosis</subject><subject>Ki-67 Antigen</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Scanning</subject><subject>Middle Aged</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - ultrastructure</subject><subject>Neoplasm Proteins - analysis</subject><subject>Nuclear Proteins - analysis</subject><subject>Proliferating Cell Nuclear Antigen - analysis</subject><subject>saphenous vein</subject><subject>Saphenous Vein - cytology</subject><subject>Saphenous Vein - transplantation</subject><subject>smooth muscle cells</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the heart</subject><subject>thrombosis</subject><subject>Thrombosis - pathology</subject><subject>Transplantation, Autologous</subject><subject>Tunica Intima - ultrastructure</subject><issn>0309-0167</issn><issn>1365-2559</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkFGL1DAUhYso67j6E4Qg4ltrkts0iQ-CLO7OyrIKrsxjSDOpk7FtatLq7L83pWXezUsg57vnnpwse0NwQdJ5fywIVCynjMmCSFkWY40JMFacnmSbs_Q022DAMsek4s-zFzEeMSYcKL3ILrgAJkq6yX5ce90hY9sWBTu0zujR-R7pfo9i5_14QN0UTWsXRA9-GH10EbkeHaZO9yjq4WB7P0X0x6bHn0E3Y3yZPWt0G-2r9b5Mez4_XG3zu683t1ef7nLDMGU5gKFcGEIkFaVkooLGNGVN5Z6bpraWQbkHXgsOgpmKlSWTQBsiBOdWA6vhMnu3-A7B_55sHFXn4pxU9zZFUpxXkpWVSOCHBTTBxxhso4bgOh0eFcFq7lQd1VycmotTc6dq7VSd0vDrdctUd3Z_Hl1LTPrbVdfR6LYJujcunjGA9L9KJuzjgv11rX38jwBqe_s9SckgXwxcHO3pbKDDL1Vx4Ezt7m_U9nr3bXe_fVBf4B9_gaKv</recordid><startdate>199410</startdate><enddate>199410</enddate><creator>KOCKX, M.M.</creator><creator>CAMBIER, B.A.</creator><creator>BORTIER, H.E.</creator><creator>MEYER, G.R. DE</creator><creator>DECLERCQ, S.C.</creator><creator>CAUWELAERT, P.A. VAN</creator><creator>BULTINCK, J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199410</creationdate><title>Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts</title><author>KOCKX, M.M. ; CAMBIER, B.A. ; BORTIER, H.E. ; MEYER, G.R. DE ; DECLERCQ, S.C. ; CAUWELAERT, P.A. VAN ; BULTINCK, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5025-33c278c11928495863fcf4b29d7cfbee534d37b87385c65445932f18877ea35b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Coronary Artery Bypass</topic><topic>Endothelium, Vascular - ultrastructure</topic><topic>foam cells</topic><topic>Foam Cells - chemistry</topic><topic>Foam Cells - cytology</topic><topic>Foam Cells - ultrastructure</topic><topic>Graft Occlusion, Vascular - pathology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Keywords: coronary artery bypass</topic><topic>Keywords: coronary artery bypass, saphenous vein, foam cells, smooth muscle cells, apoptosis, thrombosis</topic><topic>Ki-67 Antigen</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Microscopy, Electron, Scanning</topic><topic>Middle Aged</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - ultrastructure</topic><topic>Neoplasm Proteins - analysis</topic><topic>Nuclear Proteins - analysis</topic><topic>Proliferating Cell Nuclear Antigen - analysis</topic><topic>saphenous vein</topic><topic>Saphenous Vein - cytology</topic><topic>Saphenous Vein - transplantation</topic><topic>smooth muscle cells</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the heart</topic><topic>thrombosis</topic><topic>Thrombosis - pathology</topic><topic>Transplantation, Autologous</topic><topic>Tunica Intima - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KOCKX, M.M.</creatorcontrib><creatorcontrib>CAMBIER, B.A.</creatorcontrib><creatorcontrib>BORTIER, H.E.</creatorcontrib><creatorcontrib>MEYER, G.R. DE</creatorcontrib><creatorcontrib>DECLERCQ, S.C.</creatorcontrib><creatorcontrib>CAUWELAERT, P.A. VAN</creatorcontrib><creatorcontrib>BULTINCK, J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Histopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KOCKX, M.M.</au><au>CAMBIER, B.A.</au><au>BORTIER, H.E.</au><au>MEYER, G.R. DE</au><au>DECLERCQ, S.C.</au><au>CAUWELAERT, P.A. VAN</au><au>BULTINCK, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts</atitle><jtitle>Histopathology</jtitle><addtitle>Histopathology</addtitle><date>1994-10</date><risdate>1994</risdate><volume>25</volume><issue>4</issue><spage>365</spage><epage>371</epage><pages>365-371</pages><issn>0309-0167</issn><eissn>1365-2559</eissn><abstract>Occlusion of saphenous vein grafts is a major problem after coronary artery bypass grafting. Segments of occluded and suboccluded implanted aortocoronary grafts were obtained during re‐intervention bypass grafting in 47 patients yielding a total of 80 vein grafts. The grafts were studied by immunohistochemistry for smooth muscle cells (ÉL‐SMC actin), macrophages (HAM56), cell replication (PCNA, Ki‐67) and transmission and scanning electronmicroscopy (TEM, SEM). In 81% of the examined grafts the (sub)occlusion was due to a myo‐intimal thickening and an associated luminal accumulation of foam cells and mural thrombi. The foam cells were constantly found at the luminal site of the myo‐intimal thickening and within the luminal part of adherent thrombi. Transmission electronmicroscopy demonstrated phagocytosis of platelets and platelet fragments by the foam cells. A significant fraction of the foam cells demonstrated nuclear immunoreactivity for Ki‐67 and PCNA. The myo‐intimal thickening of the vein grafts was composed of smooth muscle cells lying in a fibrous tissue matrix. The smooth muscle cells were surrounded by prominent basal lamina and showed ultrastructural features of apoptosis. Our results support the hypothesis that phagocytosis of lipid rich platelets by monocytes set up a mechanism for foam cell formation and replication in human saphenous vein grafts. The transformation of a smooth muscle cell rich myo‐intimal thickening towards a fibrous, cell poor intimal thickening could be induced by progressive smooth muscle cell loss through apoptosis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7835842</pmid><doi>10.1111/j.1365-2559.1994.tb01355.x</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0309-0167 |
ispartof | Histopathology, 1994-10, Vol.25 (4), p.365-371 |
issn | 0309-0167 1365-2559 |
language | eng |
recordid | cdi_proquest_miscellaneous_77695468 |
source | Wiley-Blackwell Journals; MEDLINE |
subjects | Apoptosis Biological and medical sciences Cell Division Coronary Artery Bypass Endothelium, Vascular - ultrastructure foam cells Foam Cells - chemistry Foam Cells - cytology Foam Cells - ultrastructure Graft Occlusion, Vascular - pathology Humans Immunohistochemistry Keywords: coronary artery bypass Keywords: coronary artery bypass, saphenous vein, foam cells, smooth muscle cells, apoptosis, thrombosis Ki-67 Antigen Medical sciences Microscopy, Electron Microscopy, Electron, Scanning Middle Aged Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - ultrastructure Neoplasm Proteins - analysis Nuclear Proteins - analysis Proliferating Cell Nuclear Antigen - analysis saphenous vein Saphenous Vein - cytology Saphenous Vein - transplantation smooth muscle cells Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the heart thrombosis Thrombosis - pathology Transplantation, Autologous Tunica Intima - ultrastructure |
title | Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T06%3A53%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Foam%20cell%20replication%20and%20smooth%20muscle%20cell%20apoptosis%20in%20human%20saphenous%20vein%20grafts&rft.jtitle=Histopathology&rft.au=KOCKX,%20M.M.&rft.date=1994-10&rft.volume=25&rft.issue=4&rft.spage=365&rft.epage=371&rft.pages=365-371&rft.issn=0309-0167&rft.eissn=1365-2559&rft_id=info:doi/10.1111/j.1365-2559.1994.tb01355.x&rft_dat=%3Cproquest_cross%3E77695468%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77695468&rft_id=info:pmid/7835842&rfr_iscdi=true |