Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts

Occlusion of saphenous vein grafts is a major problem after coronary artery bypass grafting. Segments of occluded and suboccluded implanted aortocoronary grafts were obtained during re‐intervention bypass grafting in 47 patients yielding a total of 80 vein grafts. The grafts were studied by immunohi...

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Veröffentlicht in:Histopathology 1994-10, Vol.25 (4), p.365-371
Hauptverfasser: KOCKX, M.M., CAMBIER, B.A., BORTIER, H.E., MEYER, G.R. DE, DECLERCQ, S.C., CAUWELAERT, P.A. VAN, BULTINCK, J.
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container_end_page 371
container_issue 4
container_start_page 365
container_title Histopathology
container_volume 25
creator KOCKX, M.M.
CAMBIER, B.A.
BORTIER, H.E.
MEYER, G.R. DE
DECLERCQ, S.C.
CAUWELAERT, P.A. VAN
BULTINCK, J.
description Occlusion of saphenous vein grafts is a major problem after coronary artery bypass grafting. Segments of occluded and suboccluded implanted aortocoronary grafts were obtained during re‐intervention bypass grafting in 47 patients yielding a total of 80 vein grafts. The grafts were studied by immunohistochemistry for smooth muscle cells (ÉL‐SMC actin), macrophages (HAM56), cell replication (PCNA, Ki‐67) and transmission and scanning electronmicroscopy (TEM, SEM). In 81% of the examined grafts the (sub)occlusion was due to a myo‐intimal thickening and an associated luminal accumulation of foam cells and mural thrombi. The foam cells were constantly found at the luminal site of the myo‐intimal thickening and within the luminal part of adherent thrombi. Transmission electronmicroscopy demonstrated phagocytosis of platelets and platelet fragments by the foam cells. A significant fraction of the foam cells demonstrated nuclear immunoreactivity for Ki‐67 and PCNA. The myo‐intimal thickening of the vein grafts was composed of smooth muscle cells lying in a fibrous tissue matrix. The smooth muscle cells were surrounded by prominent basal lamina and showed ultrastructural features of apoptosis. Our results support the hypothesis that phagocytosis of lipid rich platelets by monocytes set up a mechanism for foam cell formation and replication in human saphenous vein grafts. The transformation of a smooth muscle cell rich myo‐intimal thickening towards a fibrous, cell poor intimal thickening could be induced by progressive smooth muscle cell loss through apoptosis.
doi_str_mv 10.1111/j.1365-2559.1994.tb01355.x
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Transmission electronmicroscopy demonstrated phagocytosis of platelets and platelet fragments by the foam cells. A significant fraction of the foam cells demonstrated nuclear immunoreactivity for Ki‐67 and PCNA. The myo‐intimal thickening of the vein grafts was composed of smooth muscle cells lying in a fibrous tissue matrix. The smooth muscle cells were surrounded by prominent basal lamina and showed ultrastructural features of apoptosis. Our results support the hypothesis that phagocytosis of lipid rich platelets by monocytes set up a mechanism for foam cell formation and replication in human saphenous vein grafts. 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DE</creatorcontrib><creatorcontrib>DECLERCQ, S.C.</creatorcontrib><creatorcontrib>CAUWELAERT, P.A. VAN</creatorcontrib><creatorcontrib>BULTINCK, J.</creatorcontrib><title>Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts</title><title>Histopathology</title><addtitle>Histopathology</addtitle><description>Occlusion of saphenous vein grafts is a major problem after coronary artery bypass grafting. Segments of occluded and suboccluded implanted aortocoronary grafts were obtained during re‐intervention bypass grafting in 47 patients yielding a total of 80 vein grafts. The grafts were studied by immunohistochemistry for smooth muscle cells (ÉL‐SMC actin), macrophages (HAM56), cell replication (PCNA, Ki‐67) and transmission and scanning electronmicroscopy (TEM, SEM). In 81% of the examined grafts the (sub)occlusion was due to a myo‐intimal thickening and an associated luminal accumulation of foam cells and mural thrombi. The foam cells were constantly found at the luminal site of the myo‐intimal thickening and within the luminal part of adherent thrombi. Transmission electronmicroscopy demonstrated phagocytosis of platelets and platelet fragments by the foam cells. A significant fraction of the foam cells demonstrated nuclear immunoreactivity for Ki‐67 and PCNA. The myo‐intimal thickening of the vein grafts was composed of smooth muscle cells lying in a fibrous tissue matrix. The smooth muscle cells were surrounded by prominent basal lamina and showed ultrastructural features of apoptosis. Our results support the hypothesis that phagocytosis of lipid rich platelets by monocytes set up a mechanism for foam cell formation and replication in human saphenous vein grafts. The transformation of a smooth muscle cell rich myo‐intimal thickening towards a fibrous, cell poor intimal thickening could be induced by progressive smooth muscle cell loss through apoptosis.</description><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Coronary Artery Bypass</subject><subject>Endothelium, Vascular - ultrastructure</subject><subject>foam cells</subject><subject>Foam Cells - chemistry</subject><subject>Foam Cells - cytology</subject><subject>Foam Cells - ultrastructure</subject><subject>Graft Occlusion, Vascular - pathology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Keywords: coronary artery bypass</subject><subject>Keywords: coronary artery bypass, saphenous vein, foam cells, smooth muscle cells, apoptosis, thrombosis</subject><subject>Ki-67 Antigen</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Scanning</subject><subject>Middle Aged</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - ultrastructure</subject><subject>Neoplasm Proteins - analysis</subject><subject>Nuclear Proteins - analysis</subject><subject>Proliferating Cell Nuclear Antigen - analysis</subject><subject>saphenous vein</subject><subject>Saphenous Vein - cytology</subject><subject>Saphenous Vein - transplantation</subject><subject>smooth muscle cells</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the heart</subject><subject>thrombosis</subject><subject>Thrombosis - pathology</subject><subject>Transplantation, Autologous</subject><subject>Tunica Intima - ultrastructure</subject><issn>0309-0167</issn><issn>1365-2559</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkFGL1DAUhYso67j6E4Qg4ltrkts0iQ-CLO7OyrIKrsxjSDOpk7FtatLq7L83pWXezUsg57vnnpwse0NwQdJ5fywIVCynjMmCSFkWY40JMFacnmSbs_Q022DAMsek4s-zFzEeMSYcKL3ILrgAJkq6yX5ce90hY9sWBTu0zujR-R7pfo9i5_14QN0UTWsXRA9-GH10EbkeHaZO9yjq4WB7P0X0x6bHn0E3Y3yZPWt0G-2r9b5Mez4_XG3zu683t1ef7nLDMGU5gKFcGEIkFaVkooLGNGVN5Z6bpraWQbkHXgsOgpmKlSWTQBsiBOdWA6vhMnu3-A7B_55sHFXn4pxU9zZFUpxXkpWVSOCHBTTBxxhso4bgOh0eFcFq7lQd1VycmotTc6dq7VSd0vDrdctUd3Z_Hl1LTPrbVdfR6LYJujcunjGA9L9KJuzjgv11rX38jwBqe_s9SckgXwxcHO3pbKDDL1Vx4Ezt7m_U9nr3bXe_fVBf4B9_gaKv</recordid><startdate>199410</startdate><enddate>199410</enddate><creator>KOCKX, M.M.</creator><creator>CAMBIER, B.A.</creator><creator>BORTIER, H.E.</creator><creator>MEYER, G.R. DE</creator><creator>DECLERCQ, S.C.</creator><creator>CAUWELAERT, P.A. VAN</creator><creator>BULTINCK, J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199410</creationdate><title>Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts</title><author>KOCKX, M.M. ; CAMBIER, B.A. ; BORTIER, H.E. ; MEYER, G.R. DE ; DECLERCQ, S.C. ; CAUWELAERT, P.A. VAN ; BULTINCK, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5025-33c278c11928495863fcf4b29d7cfbee534d37b87385c65445932f18877ea35b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Coronary Artery Bypass</topic><topic>Endothelium, Vascular - ultrastructure</topic><topic>foam cells</topic><topic>Foam Cells - chemistry</topic><topic>Foam Cells - cytology</topic><topic>Foam Cells - ultrastructure</topic><topic>Graft Occlusion, Vascular - pathology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Keywords: coronary artery bypass</topic><topic>Keywords: coronary artery bypass, saphenous vein, foam cells, smooth muscle cells, apoptosis, thrombosis</topic><topic>Ki-67 Antigen</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Microscopy, Electron, Scanning</topic><topic>Middle Aged</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - ultrastructure</topic><topic>Neoplasm Proteins - analysis</topic><topic>Nuclear Proteins - analysis</topic><topic>Proliferating Cell Nuclear Antigen - analysis</topic><topic>saphenous vein</topic><topic>Saphenous Vein - cytology</topic><topic>Saphenous Vein - transplantation</topic><topic>smooth muscle cells</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the heart</topic><topic>thrombosis</topic><topic>Thrombosis - pathology</topic><topic>Transplantation, Autologous</topic><topic>Tunica Intima - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KOCKX, M.M.</creatorcontrib><creatorcontrib>CAMBIER, B.A.</creatorcontrib><creatorcontrib>BORTIER, H.E.</creatorcontrib><creatorcontrib>MEYER, G.R. DE</creatorcontrib><creatorcontrib>DECLERCQ, S.C.</creatorcontrib><creatorcontrib>CAUWELAERT, P.A. 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VAN</au><au>BULTINCK, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts</atitle><jtitle>Histopathology</jtitle><addtitle>Histopathology</addtitle><date>1994-10</date><risdate>1994</risdate><volume>25</volume><issue>4</issue><spage>365</spage><epage>371</epage><pages>365-371</pages><issn>0309-0167</issn><eissn>1365-2559</eissn><abstract>Occlusion of saphenous vein grafts is a major problem after coronary artery bypass grafting. Segments of occluded and suboccluded implanted aortocoronary grafts were obtained during re‐intervention bypass grafting in 47 patients yielding a total of 80 vein grafts. The grafts were studied by immunohistochemistry for smooth muscle cells (ÉL‐SMC actin), macrophages (HAM56), cell replication (PCNA, Ki‐67) and transmission and scanning electronmicroscopy (TEM, SEM). In 81% of the examined grafts the (sub)occlusion was due to a myo‐intimal thickening and an associated luminal accumulation of foam cells and mural thrombi. The foam cells were constantly found at the luminal site of the myo‐intimal thickening and within the luminal part of adherent thrombi. Transmission electronmicroscopy demonstrated phagocytosis of platelets and platelet fragments by the foam cells. A significant fraction of the foam cells demonstrated nuclear immunoreactivity for Ki‐67 and PCNA. The myo‐intimal thickening of the vein grafts was composed of smooth muscle cells lying in a fibrous tissue matrix. The smooth muscle cells were surrounded by prominent basal lamina and showed ultrastructural features of apoptosis. Our results support the hypothesis that phagocytosis of lipid rich platelets by monocytes set up a mechanism for foam cell formation and replication in human saphenous vein grafts. The transformation of a smooth muscle cell rich myo‐intimal thickening towards a fibrous, cell poor intimal thickening could be induced by progressive smooth muscle cell loss through apoptosis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7835842</pmid><doi>10.1111/j.1365-2559.1994.tb01355.x</doi><tpages>7</tpages></addata></record>
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source Wiley-Blackwell Journals; MEDLINE
subjects Apoptosis
Biological and medical sciences
Cell Division
Coronary Artery Bypass
Endothelium, Vascular - ultrastructure
foam cells
Foam Cells - chemistry
Foam Cells - cytology
Foam Cells - ultrastructure
Graft Occlusion, Vascular - pathology
Humans
Immunohistochemistry
Keywords: coronary artery bypass
Keywords: coronary artery bypass, saphenous vein, foam cells, smooth muscle cells, apoptosis, thrombosis
Ki-67 Antigen
Medical sciences
Microscopy, Electron
Microscopy, Electron, Scanning
Middle Aged
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - ultrastructure
Neoplasm Proteins - analysis
Nuclear Proteins - analysis
Proliferating Cell Nuclear Antigen - analysis
saphenous vein
Saphenous Vein - cytology
Saphenous Vein - transplantation
smooth muscle cells
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the heart
thrombosis
Thrombosis - pathology
Transplantation, Autologous
Tunica Intima - ultrastructure
title Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts
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