Isolation and characterization of ovokinin, a bradykinin B1 agonist peptide derived from ovalbumin
A vasorelaxing peptide was purified from a peptic digest of ovalbumin, after three steps of reverse-phase HPLC. The structure of the peptide was Phe-Arg-Ala-Asp-His-Pro-Phe-Leu, which corresponded to residues 358-365 of ovalbumin. The peptide was named ovokinin. Ovokinin showed relaxing activity for...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 1995, Vol.16 (5), p.785-790 |
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| container_title | Peptides (New York, N.Y. : 1980) |
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| creator | Fujita, H Usui, H Kurahashi, K Yoshikawa, M |
| description | A vasorelaxing peptide was purified from a peptic digest of ovalbumin, after three steps of reverse-phase HPLC. The structure of the peptide was Phe-Arg-Ala-Asp-His-Pro-Phe-Leu, which corresponded to residues 358-365 of ovalbumin. The peptide was named ovokinin. Ovokinin showed relaxing activity for a canine mesenteric artery (EC50 = 6.3 microM). The relaxing activity was blocked by the bradykinin B1 antagonist [des-Arg9] [Leu8]bradykinin, but not by the B2 antagonist Hoe 140. Ovokinin binds to B1 receptors (IC50 = 64 microM). Prostaglandin I2 was released from the artery after ovokinin stimulation as a relaxing factor. Thus, ovokinin is a weak bradykinin B1 agonist peptide derived from food proteins. |
| doi_str_mv | 10.1016/0196-9781(95)00054-n |
| format | Article |
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The structure of the peptide was Phe-Arg-Ala-Asp-His-Pro-Phe-Leu, which corresponded to residues 358-365 of ovalbumin. The peptide was named ovokinin. Ovokinin showed relaxing activity for a canine mesenteric artery (EC50 = 6.3 microM). The relaxing activity was blocked by the bradykinin B1 antagonist [des-Arg9] [Leu8]bradykinin, but not by the B2 antagonist Hoe 140. Ovokinin binds to B1 receptors (IC50 = 64 microM). Prostaglandin I2 was released from the artery after ovokinin stimulation as a relaxing factor. 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The structure of the peptide was Phe-Arg-Ala-Asp-His-Pro-Phe-Leu, which corresponded to residues 358-365 of ovalbumin. The peptide was named ovokinin. Ovokinin showed relaxing activity for a canine mesenteric artery (EC50 = 6.3 microM). The relaxing activity was blocked by the bradykinin B1 antagonist [des-Arg9] [Leu8]bradykinin, but not by the B2 antagonist Hoe 140. Ovokinin binds to B1 receptors (IC50 = 64 microM). Prostaglandin I2 was released from the artery after ovokinin stimulation as a relaxing factor. Thus, ovokinin is a weak bradykinin B1 agonist peptide derived from food proteins.</description><subject>1-Methyl-3-isobutylxanthine - pharmacology</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bradykinin - agonists</subject><subject>Bradykinin - antagonists & inhibitors</subject><subject>Dinoprost - pharmacology</subject><subject>Dogs</subject><subject>Dose-Response Relationship, Drug</subject><subject>Egg Proteins - chemistry</subject><subject>Egg Proteins - isolation & purification</subject><subject>Egg Proteins - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Mesenteric Arteries - drug effects</subject><subject>Mesenteric Arteries - physiology</subject><subject>Molecular Sequence Data</subject><subject>Muscle Relaxation - drug effects</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - physiology</subject><subject>Ovalbumin - chemistry</subject><subject>Papaverine - pharmacology</subject><subject>Peptide Fragments - chemistry</subject><subject>Peptide Fragments - isolation & purification</subject><subject>Peptide Fragments - pharmacology</subject><subject>Phosphodiesterase Inhibitors - pharmacology</subject><subject>Purinones - pharmacology</subject><subject>Pyrrolidinones - pharmacology</subject><subject>Rolipram</subject><subject>Vasodilator Agents - isolation & purification</subject><subject>Vasodilator Agents - pharmacology</subject><issn>0196-9781</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRb0AFSj8AUheIZAI2HHsxEuoeFSqYANry_EDDIkd7KRS-XrSh7oazZ05d3EAOMfoFiPM7hDmLONlha84vUYI0SLzB-B4Hx-Bk5S-x0NR8GoCJmVRcoLZMajnKTSyd8FD6TVUXzJK1Zvo_rZhsDAsw4_zzt9ACeso9WqzwQcM5WfwLvWwM13vtIF65JZGQxtDO2KyqYfW-VNwaGWTzNluTsHH0-P77CVbvD3PZ_eLTOUV6TNG86JkVFGieE1IYUuMc6kwo7ag1GrNc66R0tRgRJjCSqOcWFJRxmrELCFTcLnt7WL4HUzqReuSMk0jvQlDEmXJOGEjNAXF9lHFkFI0VnTRtTKuBEZirVOsvYm1N8Gp2OgUryN2sesf6tboPbRzSf4BCnJzBQ</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Fujita, H</creator><creator>Usui, H</creator><creator>Kurahashi, K</creator><creator>Yoshikawa, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Isolation and characterization of ovokinin, a bradykinin B1 agonist peptide derived from ovalbumin</title><author>Fujita, H ; Usui, H ; Kurahashi, K ; Yoshikawa, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c283t-6524765c53c9b334f7112ac165f455fdd929d0cd5e1036c1cd023f38566b06f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>1-Methyl-3-isobutylxanthine - pharmacology</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bradykinin - agonists</topic><topic>Bradykinin - antagonists & inhibitors</topic><topic>Dinoprost - pharmacology</topic><topic>Dogs</topic><topic>Dose-Response Relationship, Drug</topic><topic>Egg Proteins - chemistry</topic><topic>Egg Proteins - isolation & purification</topic><topic>Egg Proteins - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Mesenteric Arteries - drug effects</topic><topic>Mesenteric Arteries - physiology</topic><topic>Molecular Sequence Data</topic><topic>Muscle Relaxation - drug effects</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - physiology</topic><topic>Ovalbumin - chemistry</topic><topic>Papaverine - pharmacology</topic><topic>Peptide Fragments - chemistry</topic><topic>Peptide Fragments - isolation & purification</topic><topic>Peptide Fragments - pharmacology</topic><topic>Phosphodiesterase Inhibitors - pharmacology</topic><topic>Purinones - pharmacology</topic><topic>Pyrrolidinones - pharmacology</topic><topic>Rolipram</topic><topic>Vasodilator Agents - isolation & purification</topic><topic>Vasodilator Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujita, H</creatorcontrib><creatorcontrib>Usui, H</creatorcontrib><creatorcontrib>Kurahashi, K</creatorcontrib><creatorcontrib>Yoshikawa, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujita, H</au><au>Usui, H</au><au>Kurahashi, K</au><au>Yoshikawa, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of ovokinin, a bradykinin B1 agonist peptide derived from ovalbumin</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>1995</date><risdate>1995</risdate><volume>16</volume><issue>5</issue><spage>785</spage><epage>790</epage><pages>785-790</pages><issn>0196-9781</issn><abstract>A vasorelaxing peptide was purified from a peptic digest of ovalbumin, after three steps of reverse-phase HPLC. The structure of the peptide was Phe-Arg-Ala-Asp-His-Pro-Phe-Leu, which corresponded to residues 358-365 of ovalbumin. The peptide was named ovokinin. Ovokinin showed relaxing activity for a canine mesenteric artery (EC50 = 6.3 microM). The relaxing activity was blocked by the bradykinin B1 antagonist [des-Arg9] [Leu8]bradykinin, but not by the B2 antagonist Hoe 140. Ovokinin binds to B1 receptors (IC50 = 64 microM). Prostaglandin I2 was released from the artery after ovokinin stimulation as a relaxing factor. Thus, ovokinin is a weak bradykinin B1 agonist peptide derived from food proteins.</abstract><cop>United States</cop><pmid>7479316</pmid><doi>10.1016/0196-9781(95)00054-n</doi><tpages>6</tpages></addata></record> |
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| ispartof | Peptides (New York, N.Y. : 1980), 1995, Vol.16 (5), p.785-790 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 1-Methyl-3-isobutylxanthine - pharmacology Amino Acid Sequence Animals Bradykinin - agonists Bradykinin - antagonists & inhibitors Dinoprost - pharmacology Dogs Dose-Response Relationship, Drug Egg Proteins - chemistry Egg Proteins - isolation & purification Egg Proteins - pharmacology In Vitro Techniques Mesenteric Arteries - drug effects Mesenteric Arteries - physiology Molecular Sequence Data Muscle Relaxation - drug effects Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - physiology Ovalbumin - chemistry Papaverine - pharmacology Peptide Fragments - chemistry Peptide Fragments - isolation & purification Peptide Fragments - pharmacology Phosphodiesterase Inhibitors - pharmacology Purinones - pharmacology Pyrrolidinones - pharmacology Rolipram Vasodilator Agents - isolation & purification Vasodilator Agents - pharmacology |
| title | Isolation and characterization of ovokinin, a bradykinin B1 agonist peptide derived from ovalbumin |
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