Crystallization and preliminary crystallographic analysis of a 2,3-dihydroxybiphenyl dioxygenase from Pseudomonas sp. strain KKS102 having polychlorinated biphenyl (PCB)-degrading activity

Crystals have been obtained for a 2,3‐dihydroxybiphenyl dioxygenase (conventionally called BphC) from a polychlorinated biphenyl (PCB)‐degrader, Pseudomonas sp. strain KKS1O2. The crystals were grown using both ammonium sulfate and MPD as the precipitating agents. The crystals belonged to a tetragon...

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Veröffentlicht in:	Proteins, structure, function, and bioinformatics structure, function, and bioinformatics, 1995-07, Vol.22 (3), p.284-286
Hauptverfasser:	Sugiyama, Kazuyuki, Narita, Hiroki, Yamamoto, Takeshi, Senda, Toshiya, Kimbara, Kazuhide, Inokuchi, Norio, Iwama, Masanori, Irie, Masachika, Fukuda, Masao, Yano, Keiji, Mitsui, Yukio
Format:	Artikel
Sprache:	eng
Schlagworte:	Crystallization Crystallography, X-Ray dioxygenase Dioxygenases Oxygenases - chemistry Oxygenases - isolation & purification PCB degrading enzyme polychlorinated biphenyl Polychlorinated Biphenyls - metabolism Pseudomonas - enzymology Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification selenomethionine
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Zusammenfassung:	Crystals have been obtained for a 2,3‐dihydroxybiphenyl dioxygenase (conventionally called BphC) from a polychlorinated biphenyl (PCB)‐degrader, Pseudomonas sp. strain KKS1O2. The crystals were grown using both ammonium sulfate and MPD as the precipitating agents. The crystals belonged to a tetragonal space group (I422) and diffracted to 2.5 Å. © 1995 Wiley‐Liss, Inc.
ISSN:	0887-3585 1097-0134
DOI:	10.1002/prot.340220309