Production of monoclonal antibodies to Bruton's tyrosine kinase
Bruton's X-linked agammaglobulinemia is caused by mutations in a cytoplasmic protein tyrosine kinase termed Bruton's tyrosine kinase (BTK). The protein is expressed in all members of the B cell lineage and is critical for B cell development. The protein consists of several modules, includi...
Gespeichert in:
| Veröffentlicht in: | Hybridoma 1995-06, Vol.14 (3), p.243-246 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 246 |
|---|---|
| container_issue | 3 |
| container_start_page | 243 |
| container_title | Hybridoma |
| container_volume | 14 |
| creator | STEWART, D. M KURMAN, C. C NELSON, D. L |
| description | Bruton's X-linked agammaglobulinemia is caused by mutations in a cytoplasmic protein tyrosine kinase termed Bruton's tyrosine kinase (BTK). The protein is expressed in all members of the B cell lineage and is critical for B cell development. The protein consists of several modules, including a pleckstrin homology domain and the Src homology domains SH1, SH2, and SH3. We report here the production of monoclonal antibodies against the pleckstrin homology domain of human BTK. The antibody was produced by immunizing mice with a FLAG-BTK fusion protein. Hybridoma supernatants were screened by ELISA using a GST-BTK fusion protein as the antigen. Selected monoclonal antibodies recognize denatured BTK on Western blots of peripheral blood mononuclear cell lysates. Mouse BTK protein is also detected. These antibodies should be useful in assessing patients with immune deficiency, as well as in studying normal B cell development. |
| doi_str_mv | 10.1089/hyb.1995.14.243 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77691580</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>77691580</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-410f11bf826beff4dd60d0d3c2b58898da75b4c1316acdbcd19ae2e377544e3d3</originalsourceid><addsrcrecordid>eNqFkD1PwzAQhi0EKqUwMyFlQDAl9fkjticEFV9SJRhAYrMc2xGB1C5xMvTfE9SqK9Od9D73SvcgdA64ACzV_HNTFaAUL4AVhNEDNCVQylxIJQ7RFBNBcsbFxzE6SekLY8wJExM0EVxhIcspunntohts38SQxTpbxRBtG4NpMxP6poqu8SnrY3bXDX0M1-O-6WJqgs--m2CSP0VHtWmTP9vNGXp_uH9bPOXLl8fnxe0yt5STPmeAa4CqlqSsfF0z50rssKOWVFxKJZ0RvGIWKJTGuso6UMYTT4XgjHnq6AxdbXvXXfwZfOr1qknWt60JPg5JC1Eq4BL_C4LAoCgWIzjfgnZ8KHW-1uuuWZluowHrP7d6dKv_3GpgenQ7Xlzsqodq5d2e38kc88tdbpI1bd2ZYJu0x2iJpQRMfwHmZ4Jq</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17019307</pqid></control><display><type>article</type><title>Production of monoclonal antibodies to Bruton's tyrosine kinase</title><source>Mary Ann Liebert Online Subscription</source><source>MEDLINE</source><creator>STEWART, D. M ; KURMAN, C. C ; NELSON, D. L</creator><creatorcontrib>STEWART, D. M ; KURMAN, C. C ; NELSON, D. L</creatorcontrib><description>Bruton's X-linked agammaglobulinemia is caused by mutations in a cytoplasmic protein tyrosine kinase termed Bruton's tyrosine kinase (BTK). The protein is expressed in all members of the B cell lineage and is critical for B cell development. The protein consists of several modules, including a pleckstrin homology domain and the Src homology domains SH1, SH2, and SH3. We report here the production of monoclonal antibodies against the pleckstrin homology domain of human BTK. The antibody was produced by immunizing mice with a FLAG-BTK fusion protein. Hybridoma supernatants were screened by ELISA using a GST-BTK fusion protein as the antigen. Selected monoclonal antibodies recognize denatured BTK on Western blots of peripheral blood mononuclear cell lysates. Mouse BTK protein is also detected. These antibodies should be useful in assessing patients with immune deficiency, as well as in studying normal B cell development.</description><identifier>ISSN: 0272-457X</identifier><identifier>EISSN: 2168-7897</identifier><identifier>EISSN: 0272-457X</identifier><identifier>DOI: 10.1089/hyb.1995.14.243</identifier><identifier>PMID: 7590786</identifier><identifier>CODEN: HYBRDY</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>Agammaglobulinemia - enzymology ; Agammaglobulinemia - immunology ; Animals ; Antibodies, immunoglobulins ; Antibodies, Monoclonal - biosynthesis ; B-Lymphocytes - enzymology ; Base Sequence ; Biological and medical sciences ; Blood Proteins - chemistry ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Mice ; Mice, Inbred BALB C ; Molecular immunology ; Molecular Sequence Data ; Monoclonal antibodies ; Phosphoproteins ; Protein-Tyrosine Kinases - chemistry ; Protein-Tyrosine Kinases - immunology ; Sequence Homology, Amino Acid</subject><ispartof>Hybridoma, 1995-06, Vol.14 (3), p.243-246</ispartof><rights>1995 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-410f11bf826beff4dd60d0d3c2b58898da75b4c1316acdbcd19ae2e377544e3d3</citedby><cites>FETCH-LOGICAL-c352t-410f11bf826beff4dd60d0d3c2b58898da75b4c1316acdbcd19ae2e377544e3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3042,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3608810$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7590786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>STEWART, D. M</creatorcontrib><creatorcontrib>KURMAN, C. C</creatorcontrib><creatorcontrib>NELSON, D. L</creatorcontrib><title>Production of monoclonal antibodies to Bruton's tyrosine kinase</title><title>Hybridoma</title><addtitle>Hybridoma</addtitle><description>Bruton's X-linked agammaglobulinemia is caused by mutations in a cytoplasmic protein tyrosine kinase termed Bruton's tyrosine kinase (BTK). The protein is expressed in all members of the B cell lineage and is critical for B cell development. The protein consists of several modules, including a pleckstrin homology domain and the Src homology domains SH1, SH2, and SH3. We report here the production of monoclonal antibodies against the pleckstrin homology domain of human BTK. The antibody was produced by immunizing mice with a FLAG-BTK fusion protein. Hybridoma supernatants were screened by ELISA using a GST-BTK fusion protein as the antigen. Selected monoclonal antibodies recognize denatured BTK on Western blots of peripheral blood mononuclear cell lysates. Mouse BTK protein is also detected. These antibodies should be useful in assessing patients with immune deficiency, as well as in studying normal B cell development.</description><subject>Agammaglobulinemia - enzymology</subject><subject>Agammaglobulinemia - immunology</subject><subject>Animals</subject><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>B-Lymphocytes - enzymology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blood Proteins - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular immunology</subject><subject>Molecular Sequence Data</subject><subject>Monoclonal antibodies</subject><subject>Phosphoproteins</subject><subject>Protein-Tyrosine Kinases - chemistry</subject><subject>Protein-Tyrosine Kinases - immunology</subject><subject>Sequence Homology, Amino Acid</subject><issn>0272-457X</issn><issn>2168-7897</issn><issn>0272-457X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EKqUwMyFlQDAl9fkjticEFV9SJRhAYrMc2xGB1C5xMvTfE9SqK9Od9D73SvcgdA64ACzV_HNTFaAUL4AVhNEDNCVQylxIJQ7RFBNBcsbFxzE6SekLY8wJExM0EVxhIcspunntohts38SQxTpbxRBtG4NpMxP6poqu8SnrY3bXDX0M1-O-6WJqgs--m2CSP0VHtWmTP9vNGXp_uH9bPOXLl8fnxe0yt5STPmeAa4CqlqSsfF0z50rssKOWVFxKJZ0RvGIWKJTGuso6UMYTT4XgjHnq6AxdbXvXXfwZfOr1qknWt60JPg5JC1Eq4BL_C4LAoCgWIzjfgnZ8KHW-1uuuWZluowHrP7d6dKv_3GpgenQ7Xlzsqodq5d2e38kc88tdbpI1bd2ZYJu0x2iJpQRMfwHmZ4Jq</recordid><startdate>19950601</startdate><enddate>19950601</enddate><creator>STEWART, D. M</creator><creator>KURMAN, C. C</creator><creator>NELSON, D. L</creator><general>Liebert</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19950601</creationdate><title>Production of monoclonal antibodies to Bruton's tyrosine kinase</title><author>STEWART, D. M ; KURMAN, C. C ; NELSON, D. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-410f11bf826beff4dd60d0d3c2b58898da75b4c1316acdbcd19ae2e377544e3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Agammaglobulinemia - enzymology</topic><topic>Agammaglobulinemia - immunology</topic><topic>Animals</topic><topic>Antibodies, immunoglobulins</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>B-Lymphocytes - enzymology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blood Proteins - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular immunology</topic><topic>Molecular Sequence Data</topic><topic>Monoclonal antibodies</topic><topic>Phosphoproteins</topic><topic>Protein-Tyrosine Kinases - chemistry</topic><topic>Protein-Tyrosine Kinases - immunology</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>online_resources</toplevel><creatorcontrib>STEWART, D. M</creatorcontrib><creatorcontrib>KURMAN, C. C</creatorcontrib><creatorcontrib>NELSON, D. L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Hybridoma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>STEWART, D. M</au><au>KURMAN, C. C</au><au>NELSON, D. L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of monoclonal antibodies to Bruton's tyrosine kinase</atitle><jtitle>Hybridoma</jtitle><addtitle>Hybridoma</addtitle><date>1995-06-01</date><risdate>1995</risdate><volume>14</volume><issue>3</issue><spage>243</spage><epage>246</epage><pages>243-246</pages><issn>0272-457X</issn><eissn>2168-7897</eissn><eissn>0272-457X</eissn><coden>HYBRDY</coden><abstract>Bruton's X-linked agammaglobulinemia is caused by mutations in a cytoplasmic protein tyrosine kinase termed Bruton's tyrosine kinase (BTK). The protein is expressed in all members of the B cell lineage and is critical for B cell development. The protein consists of several modules, including a pleckstrin homology domain and the Src homology domains SH1, SH2, and SH3. We report here the production of monoclonal antibodies against the pleckstrin homology domain of human BTK. The antibody was produced by immunizing mice with a FLAG-BTK fusion protein. Hybridoma supernatants were screened by ELISA using a GST-BTK fusion protein as the antigen. Selected monoclonal antibodies recognize denatured BTK on Western blots of peripheral blood mononuclear cell lysates. Mouse BTK protein is also detected. These antibodies should be useful in assessing patients with immune deficiency, as well as in studying normal B cell development.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>7590786</pmid><doi>10.1089/hyb.1995.14.243</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0272-457X |
| ispartof | Hybridoma, 1995-06, Vol.14 (3), p.243-246 |
| issn | 0272-457X 2168-7897 0272-457X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77691580 |
| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Agammaglobulinemia - enzymology Agammaglobulinemia - immunology Animals Antibodies, immunoglobulins Antibodies, Monoclonal - biosynthesis B-Lymphocytes - enzymology Base Sequence Biological and medical sciences Blood Proteins - chemistry Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Mice Mice, Inbred BALB C Molecular immunology Molecular Sequence Data Monoclonal antibodies Phosphoproteins Protein-Tyrosine Kinases - chemistry Protein-Tyrosine Kinases - immunology Sequence Homology, Amino Acid |
| title | Production of monoclonal antibodies to Bruton's tyrosine kinase |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T01%3A01%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Production%20of%20monoclonal%20antibodies%20to%20Bruton's%20tyrosine%20kinase&rft.jtitle=Hybridoma&rft.au=STEWART,%20D.%20M&rft.date=1995-06-01&rft.volume=14&rft.issue=3&rft.spage=243&rft.epage=246&rft.pages=243-246&rft.issn=0272-457X&rft.eissn=2168-7897&rft.coden=HYBRDY&rft_id=info:doi/10.1089/hyb.1995.14.243&rft_dat=%3Cproquest_cross%3E77691580%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17019307&rft_id=info:pmid/7590786&rfr_iscdi=true |