Proteoglycan Synthesis by Corneal Explants from Developing Embryonic Chicken

Proteoglycan biosynthesis in developing chicken corneas was studied. Corneas free of scleral rims were prepared from 5- to 18-day-old chick embryos and labeled in vitro with [3H]glucosamine and [35S]sulfate. Then, the labeled medium and corneal proteoglycans were analyzed. Day 5 corneas synthesized...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1995-04, Vol.117 (4), p.707-718
Hauptverfasser:	Nakazawa, Kiyoshi, Suzuki, Shigeo, Wada, Kaoru, Nakazawa, Kaori
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chick Embryo chondroitin sulfate/dermatan sulfate Chromatography, High Pressure Liquid Chromatography, Ion Exchange Cornea - chemistry Cornea - embryology Cornea - metabolism corneal development corneal explant Culture Techniques Ethanolamines Glucosamine - metabolism Glycosaminoglycans - analysis keratan sulfate Macromolecular Substances Proteoglycans - biosynthesis Proteoglycans - isolation & purification sulfation of proteoglycans Sulfur Radioisotopes Tritium
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