Sleep deprivation differentially alters the mRNA and protein levels of neurogranin in rat brain
The mRNA level of the 17-kDa protein neurogranin (NG), a postsynaptic substrate of the protein kinase C, has previously been found to be decreased in rat forebrain after 24-h sleep deprivation (SD). To investigate the functional significance of this finding in various forebrain regions, the effect o...
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| Veröffentlicht in: | Brain research 1995-07, Vol.685 (1), p.143-153 |
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| creator | Neuner-Jehle, Martin Rhyner, Thomas A. Borbély, Alexander A. |
| description | The mRNA level of the 17-kDa protein neurogranin (NG), a postsynaptic substrate of the protein kinase C, has previously been found to be decreased in rat forebrain after 24-h sleep deprivation (SD). To investigate the functional significance of this finding in various forebrain regions, the effect of 24-h SD on the mRNA level and the protein level of NG was determined in the cerebral cortex, hippocampus, and the total of the remaining subcortical forebrain plus midbrain areas (SFMA) of rats. In these areas, high levels of both NG mRNA and NG protein were detected by in situ hybridization and immunohistochemistry, respectively. NG protein was recognized in brain tissue by newly developed polyclonal antibodies. As determined by RNase protection assats, the level of NG mRNA was decreased in SFMA by 34 ± 7% (
P < 0.05) after 24-h SD, and was not significantly affected in the cerebral cortex and hippocampus. In contrast, on Western blots, the protein concentration of NG was reduced in the cerebral cortex by 37 ± 7% (
P < 0.05) whereas no significant changes were present in other brain areas tested. The results indicate that the mRNA and protein levels of NG are differentially modulated in rat brain by the prolongation of the waking period. |
| doi_str_mv | 10.1016/0006-8993(95)00416-N |
| format | Article |
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P < 0.05) after 24-h SD, and was not significantly affected in the cerebral cortex and hippocampus. In contrast, on Western blots, the protein concentration of NG was reduced in the cerebral cortex by 37 ± 7% (
P < 0.05) whereas no significant changes were present in other brain areas tested. The results indicate that the mRNA and protein levels of NG are differentially modulated in rat brain by the prolongation of the waking period.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(95)00416-N</identifier><identifier>PMID: 7583240</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Brain - metabolism ; Calmodulin-Binding Proteins - genetics ; Calmodulin-Binding Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Male ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Neurogranin ; Neurogranin mRNA ; Rats ; Rats, Sprague-Dawley ; RC3 ; RNA, Messenger - biosynthesis ; Sleep deprivation ; Sleep Deprivation - physiology ; Sleep regulation ; Sleep. Vigilance ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1995-07, Vol.685 (1), p.143-153</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-863bb67be802e771f27dd391345a79bf30f98c928ac28c1a9a2acc2200042dcd3</citedby><cites>FETCH-LOGICAL-c332t-863bb67be802e771f27dd391345a79bf30f98c928ac28c1a9a2acc2200042dcd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/000689939500416N$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3583937$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7583240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neuner-Jehle, Martin</creatorcontrib><creatorcontrib>Rhyner, Thomas A.</creatorcontrib><creatorcontrib>Borbély, Alexander A.</creatorcontrib><title>Sleep deprivation differentially alters the mRNA and protein levels of neurogranin in rat brain</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The mRNA level of the 17-kDa protein neurogranin (NG), a postsynaptic substrate of the protein kinase C, has previously been found to be decreased in rat forebrain after 24-h sleep deprivation (SD). To investigate the functional significance of this finding in various forebrain regions, the effect of 24-h SD on the mRNA level and the protein level of NG was determined in the cerebral cortex, hippocampus, and the total of the remaining subcortical forebrain plus midbrain areas (SFMA) of rats. In these areas, high levels of both NG mRNA and NG protein were detected by in situ hybridization and immunohistochemistry, respectively. NG protein was recognized in brain tissue by newly developed polyclonal antibodies. As determined by RNase protection assats, the level of NG mRNA was decreased in SFMA by 34 ± 7% (
P < 0.05) after 24-h SD, and was not significantly affected in the cerebral cortex and hippocampus. In contrast, on Western blots, the protein concentration of NG was reduced in the cerebral cortex by 37 ± 7% (
P < 0.05) whereas no significant changes were present in other brain areas tested. The results indicate that the mRNA and protein levels of NG are differentially modulated in rat brain by the prolongation of the waking period.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - metabolism</subject><subject>Calmodulin-Binding Proteins - genetics</subject><subject>Calmodulin-Binding Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurogranin</subject><subject>Neurogranin mRNA</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RC3</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Sleep deprivation</subject><subject>Sleep Deprivation - physiology</subject><subject>Sleep regulation</subject><subject>Sleep. Vigilance</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtrVDEQgIModVv9Bwp5ENGHU3M5m8uLUIq1QtmCl-eQk0w0ks1Zk-xC_71Zd9lHC4GQzDfDzDcIvaLkkhIqPhBCxKC05u_08j0hIxXD6glaUCXZINhInqLFCXmOzmv93Z-ca3KGzuRS8Y4skPmWADbYw6bEnW1xztjHEKBAbtGm9IBtalAqbr8Ar7-urrDNHm_K3CBmnGAHqeI54AzbMv8sNvfffopteCo25hfoWbCpwsvjfYF-3Hz6fn073N1__nJ9dTc4zlkblODTJOQEijCQkgYmveea8nFppZ4CJ0Erp5myjilHrbbMOsdYn2hk3nl-gd4e6vbW_myhNrOO1UFKNsO8rUZKofrM_FGQCiVGycYOjgfQlbnWAsF0RWtbHgwlZr8As7dr9naNXpp_CzCrnvb6WH87rcGfko7Ge_zNMW6rsyl0ZS7WE8Y7prns2McD1v3CLkIx1UXIDnws4Jrxc_x_H38BDGWh2w</recordid><startdate>19950710</startdate><enddate>19950710</enddate><creator>Neuner-Jehle, Martin</creator><creator>Rhyner, Thomas A.</creator><creator>Borbély, Alexander A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19950710</creationdate><title>Sleep deprivation differentially alters the mRNA and protein levels of neurogranin in rat brain</title><author>Neuner-Jehle, Martin ; Rhyner, Thomas A. ; Borbély, Alexander A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-863bb67be802e771f27dd391345a79bf30f98c928ac28c1a9a2acc2200042dcd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - metabolism</topic><topic>Calmodulin-Binding Proteins - genetics</topic><topic>Calmodulin-Binding Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurogranin</topic><topic>Neurogranin mRNA</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RC3</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Sleep deprivation</topic><topic>Sleep Deprivation - physiology</topic><topic>Sleep regulation</topic><topic>Sleep. Vigilance</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neuner-Jehle, Martin</creatorcontrib><creatorcontrib>Rhyner, Thomas A.</creatorcontrib><creatorcontrib>Borbély, Alexander A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neuner-Jehle, Martin</au><au>Rhyner, Thomas A.</au><au>Borbély, Alexander A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sleep deprivation differentially alters the mRNA and protein levels of neurogranin in rat brain</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1995-07-10</date><risdate>1995</risdate><volume>685</volume><issue>1</issue><spage>143</spage><epage>153</epage><pages>143-153</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The mRNA level of the 17-kDa protein neurogranin (NG), a postsynaptic substrate of the protein kinase C, has previously been found to be decreased in rat forebrain after 24-h sleep deprivation (SD). To investigate the functional significance of this finding in various forebrain regions, the effect of 24-h SD on the mRNA level and the protein level of NG was determined in the cerebral cortex, hippocampus, and the total of the remaining subcortical forebrain plus midbrain areas (SFMA) of rats. In these areas, high levels of both NG mRNA and NG protein were detected by in situ hybridization and immunohistochemistry, respectively. NG protein was recognized in brain tissue by newly developed polyclonal antibodies. As determined by RNase protection assats, the level of NG mRNA was decreased in SFMA by 34 ± 7% (
P < 0.05) after 24-h SD, and was not significantly affected in the cerebral cortex and hippocampus. In contrast, on Western blots, the protein concentration of NG was reduced in the cerebral cortex by 37 ± 7% (
P < 0.05) whereas no significant changes were present in other brain areas tested. The results indicate that the mRNA and protein levels of NG are differentially modulated in rat brain by the prolongation of the waking period.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>7583240</pmid><doi>10.1016/0006-8993(95)00416-N</doi><tpages>11</tpages></addata></record> |
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| ispartof | Brain research, 1995-07, Vol.685 (1), p.143-153 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Brain - metabolism Calmodulin-Binding Proteins - genetics Calmodulin-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology Male Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neurogranin Neurogranin mRNA Rats Rats, Sprague-Dawley RC3 RNA, Messenger - biosynthesis Sleep deprivation Sleep Deprivation - physiology Sleep regulation Sleep. Vigilance Vertebrates: nervous system and sense organs |
| title | Sleep deprivation differentially alters the mRNA and protein levels of neurogranin in rat brain |
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