Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine
The compound 5‐azacytidine has been previously shown to convert cells of the rat embryonic fibroblastic cell line, C3H/10T1/2, into myoblasts, adipocytes, and chondrocytes. Rare, resident cells of bone marrow and periosteum, referred to as mesenchymal stem cells, have been shown to differentiate int...
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| Veröffentlicht in: | Muscle & nerve 1995-12, Vol.18 (12), p.1417-1426 |
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| container_title | Muscle & nerve |
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| creator | Wakitani, Shigeyuki Saito, Tomoyuki Caplan, Arnold I. |
| description | The compound 5‐azacytidine has been previously shown to convert cells of the rat embryonic fibroblastic cell line, C3H/10T1/2, into myoblasts, adipocytes, and chondrocytes. Rare, resident cells of bone marrow and periosteum, referred to as mesenchymal stem cells, have been shown to differentiate into a number of mesenchymal phenotypes including bone, cartilage, and adipocytes. Rat bone marrow‐derived mesenchymal stem cells were exposed to 5‐azacytidine beginning 24 h after seeding twice‐passaged cells into culture dishes. After an exposure of 24 h, long, multinucleated myotubes were observed in some of the dishes 7–11 days later. Cells containing Sudan black‐positive droplets in their cytoplasm were also observed. Thus, culture‐propagated rat bone marrow mesenchymal stem cells appear to have the capacity to be induced to differentiate in vitro into myogenic and adipocytic phenotypes, although nonmesenchymal cells (rat brain fibroblasts) cannot be so induced. Taken together, these observations provide support for the suggestion that mesenchymal stem cells in the bone marrow of postnatal organisms may provide a source for myoprogenitor cells which could function in clinically relevant myogenic regeneration. © 1995 John Wiley & Sons, Inc. |
| doi_str_mv | 10.1002/mus.880181212 |
| format | Article |
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Rare, resident cells of bone marrow and periosteum, referred to as mesenchymal stem cells, have been shown to differentiate into a number of mesenchymal phenotypes including bone, cartilage, and adipocytes. Rat bone marrow‐derived mesenchymal stem cells were exposed to 5‐azacytidine beginning 24 h after seeding twice‐passaged cells into culture dishes. After an exposure of 24 h, long, multinucleated myotubes were observed in some of the dishes 7–11 days later. Cells containing Sudan black‐positive droplets in their cytoplasm were also observed. Thus, culture‐propagated rat bone marrow mesenchymal stem cells appear to have the capacity to be induced to differentiate in vitro into myogenic and adipocytic phenotypes, although nonmesenchymal cells (rat brain fibroblasts) cannot be so induced. Taken together, these observations provide support for the suggestion that mesenchymal stem cells in the bone marrow of postnatal organisms may provide a source for myoprogenitor cells which could function in clinically relevant myogenic regeneration. © 1995 John Wiley & Sons, Inc.</description><identifier>ISSN: 0148-639X</identifier><identifier>EISSN: 1097-4598</identifier><identifier>DOI: 10.1002/mus.880181212</identifier><identifier>PMID: 7477065</identifier><identifier>CODEN: MUNEDE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>adipocytes ; Adipocytes - drug effects ; Animals ; Azacitidine - pharmacology ; Biological and medical sciences ; Bone Marrow Cells ; Cell Count ; Cell Differentiation - drug effects ; Cell differentiation, maturation, development, hematopoiesis ; cell lineage ; Cell physiology ; Cells, Cultured - drug effects ; Cloning, Molecular ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. Psychology ; Male ; Molecular and cellular biology ; muscle ; Muscles - cytology ; Rats ; Rats, Inbred F344 ; stem cells ; Stem Cells - drug effects ; Time Factors</subject><ispartof>Muscle & nerve, 1995-12, Vol.18 (12), p.1417-1426</ispartof><rights>Copyright © 1995 John Wiley & Sons, Inc.</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4692-bcdf2e44231e25db6c1f94011de7b77e7ae624d736ae06ae95f5c422b74310253</citedby><cites>FETCH-LOGICAL-c4692-bcdf2e44231e25db6c1f94011de7b77e7ae624d736ae06ae95f5c422b74310253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmus.880181212$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmus.880181212$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2927512$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7477065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wakitani, Shigeyuki</creatorcontrib><creatorcontrib>Saito, Tomoyuki</creatorcontrib><creatorcontrib>Caplan, Arnold I.</creatorcontrib><title>Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine</title><title>Muscle & nerve</title><addtitle>Muscle Nerve</addtitle><description>The compound 5‐azacytidine has been previously shown to convert cells of the rat embryonic fibroblastic cell line, C3H/10T1/2, into myoblasts, adipocytes, and chondrocytes. Rare, resident cells of bone marrow and periosteum, referred to as mesenchymal stem cells, have been shown to differentiate into a number of mesenchymal phenotypes including bone, cartilage, and adipocytes. Rat bone marrow‐derived mesenchymal stem cells were exposed to 5‐azacytidine beginning 24 h after seeding twice‐passaged cells into culture dishes. After an exposure of 24 h, long, multinucleated myotubes were observed in some of the dishes 7–11 days later. Cells containing Sudan black‐positive droplets in their cytoplasm were also observed. Thus, culture‐propagated rat bone marrow mesenchymal stem cells appear to have the capacity to be induced to differentiate in vitro into myogenic and adipocytic phenotypes, although nonmesenchymal cells (rat brain fibroblasts) cannot be so induced. Taken together, these observations provide support for the suggestion that mesenchymal stem cells in the bone marrow of postnatal organisms may provide a source for myoprogenitor cells which could function in clinically relevant myogenic regeneration. © 1995 John Wiley & Sons, Inc.</description><subject>adipocytes</subject><subject>Adipocytes - drug effects</subject><subject>Animals</subject><subject>Azacitidine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Cells</subject><subject>Cell Count</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>cell lineage</subject><subject>Cell physiology</subject><subject>Cells, Cultured - drug effects</subject><subject>Cloning, Molecular</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Molecular and cellular biology</subject><subject>muscle</subject><subject>Muscles - cytology</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>stem cells</subject><subject>Stem Cells - drug effects</subject><subject>Time Factors</subject><issn>0148-639X</issn><issn>1097-4598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1v1DAQxS0EKkvhyBHJB8Qtre3YnuSIWmhB_ZCAQiUOluNMwJDEi52lDX99XTZaceIw8mF-783zI-Q5ZwecMXE4bNJBVTFeccHFA7LirIZCqrp6SFaMy6rQZX39mDxJ6QdjGdOwR_ZAAjCtVuTr-Ry-4egdddj3ibYY_W9saRfDQKOdaBNGpIONMdzQAROO7vs82J6mCYdFg7frkLJmClQV9o918-RbP-JT8qizfcJny7tPrt6--XR0Wpxdnrw7en1WOKlrUTSu7QRKKUqOQrWNdryrJeO8RWgAECxqIVsotUWWp1adclKIBmTJmVDlPnm19V3H8GuDaTKDT_fR7IhhkwyArpRQIoPFFnQxpBSxM-vo899mw5m5L9PkMs2uzMy_WIw3zYDtjl7ay_uXy94mZ_su2tH5tMNELUD9tYEtduN7nP9_05xfffw3wBLY57pvd0obfxoNJSjz5eLEqNPj6_efP9SGlXfdc5yj</recordid><startdate>199512</startdate><enddate>199512</enddate><creator>Wakitani, Shigeyuki</creator><creator>Saito, Tomoyuki</creator><creator>Caplan, Arnold I.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199512</creationdate><title>Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine</title><author>Wakitani, Shigeyuki ; Saito, Tomoyuki ; Caplan, Arnold I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4692-bcdf2e44231e25db6c1f94011de7b77e7ae624d736ae06ae95f5c422b74310253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>adipocytes</topic><topic>Adipocytes - drug effects</topic><topic>Animals</topic><topic>Azacitidine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Cells</topic><topic>Cell Count</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>cell lineage</topic><topic>Cell physiology</topic><topic>Cells, Cultured - drug effects</topic><topic>Cloning, Molecular</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Molecular and cellular biology</topic><topic>muscle</topic><topic>Muscles - cytology</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>stem cells</topic><topic>Stem Cells - drug effects</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wakitani, Shigeyuki</creatorcontrib><creatorcontrib>Saito, Tomoyuki</creatorcontrib><creatorcontrib>Caplan, Arnold I.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Muscle & nerve</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wakitani, Shigeyuki</au><au>Saito, Tomoyuki</au><au>Caplan, Arnold I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine</atitle><jtitle>Muscle & nerve</jtitle><addtitle>Muscle Nerve</addtitle><date>1995-12</date><risdate>1995</risdate><volume>18</volume><issue>12</issue><spage>1417</spage><epage>1426</epage><pages>1417-1426</pages><issn>0148-639X</issn><eissn>1097-4598</eissn><coden>MUNEDE</coden><abstract>The compound 5‐azacytidine has been previously shown to convert cells of the rat embryonic fibroblastic cell line, C3H/10T1/2, into myoblasts, adipocytes, and chondrocytes. Rare, resident cells of bone marrow and periosteum, referred to as mesenchymal stem cells, have been shown to differentiate into a number of mesenchymal phenotypes including bone, cartilage, and adipocytes. Rat bone marrow‐derived mesenchymal stem cells were exposed to 5‐azacytidine beginning 24 h after seeding twice‐passaged cells into culture dishes. After an exposure of 24 h, long, multinucleated myotubes were observed in some of the dishes 7–11 days later. Cells containing Sudan black‐positive droplets in their cytoplasm were also observed. Thus, culture‐propagated rat bone marrow mesenchymal stem cells appear to have the capacity to be induced to differentiate in vitro into myogenic and adipocytic phenotypes, although nonmesenchymal cells (rat brain fibroblasts) cannot be so induced. Taken together, these observations provide support for the suggestion that mesenchymal stem cells in the bone marrow of postnatal organisms may provide a source for myoprogenitor cells which could function in clinically relevant myogenic regeneration. © 1995 John Wiley & Sons, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7477065</pmid><doi>10.1002/mus.880181212</doi><tpages>10</tpages></addata></record> |
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| subjects | adipocytes Adipocytes - drug effects Animals Azacitidine - pharmacology Biological and medical sciences Bone Marrow Cells Cell Count Cell Differentiation - drug effects Cell differentiation, maturation, development, hematopoiesis cell lineage Cell physiology Cells, Cultured - drug effects Cloning, Molecular Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Male Molecular and cellular biology muscle Muscles - cytology Rats Rats, Inbred F344 stem cells Stem Cells - drug effects Time Factors |
| title | Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine |
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