FTIR Study of a Nonclassical dT10dA10-dT10 Intramolecular Triple Helix
Many early investigations on triple helices have been devoted to the study of the triplex formed by dT*dA-dT base triplets in which the third strand is oriented parallel to the dA strand. We now describe an intramolecular triple helix with dT*dA-dT base triplets in which the pyrimidine third strand...
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| Veröffentlicht in: | Biochemistry (Easton) 1995-11, Vol.34 (45), p.14815-14818 |
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| creator | Dagneaux, C Liquier, J Taillandier, E |
| description | Many early investigations on triple helices have been devoted to the study of the triplex formed by dT*dA-dT base triplets in which the third strand is oriented parallel to the dA strand. We now describe an intramolecular triple helix with dT*dA-dT base triplets in which the pyrimidine third strand is oriented antiparallel, formed by folding back twice the tridecamer dT10-linker-dA10-linker-dT10 (linker = pO(CH2CH2O)3p). Third-strand base pairing to the target strand, sugar conformation, and thermal denaturation of the triplex have been studied by Fourier transform infrared spectroscopy. Our results confirm than when the third-strand orientation is reversed from parallel to antiparallel with respect to the target strand, the third-strand hydrogen-bonding scheme is changed from Hoogsteen to reverse Hoogsteen. The sugar conformation in this triple helix is of the S type (C2'endo/anti, B family form) from all strands. Our results are discussed with respect to models for triplexes proposed as intermediates in homologous recombination [Zhurkin, V.B., Raghunathan, G., Ulyanov, N.B., Camerini-Otero, R.D., & Jernigan, R.L. (1994) J. Mol. Biol. 239, 181-200]. |
| doi_str_mv | 10.1021/bi00045a023 |
| format | Article |
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Biol. 239, 181-200].</description><subject>Animals</subject><subject>Base Composition</subject><subject>DNA - chemistry</subject><subject>Molecular Structure</subject><subject>Nucleic Acid Conformation</subject><subject>Poly A - chemistry</subject><subject>Poly T - chemistry</subject><subject>Spectroscopy, Fourier Transform Infrared - methods</subject><subject>Temperature</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMFLwzAUh4Moc05PnoWe9CDVJG362qMM5wZziqt4DGn6Ap3ZOpMWtv_ejo3hwdN7vN_H78FHyDWjD4xy9lhUlNJYKMqjE9JngtMwzjJxSvrdPQl5ltBzcuH9YodRiHukBwJSmtE-GY3yyUcwb9pyG9QmUMGsXmmrvK-0skGZM1o-MRrulmCyapxa1hZ1a5ULcletLQZjtNXmkpwZZT1eHeaAfI6e8-E4nL69TIZP01DxJGvCEk2RRlFmWJxojt0xLZQGkZSIwFVhTApxVCBobgSiEGB4qhCESRQIBtGA3O57167-adE3cll5jdaqFdatlwBJGqcQdeD9HtSu9t6hkWtXLZXbSkblzpr8Y62jbw61bbHE8sgeNHV5uM8r3-DmGCv3LROIQMj8fS5hFtOv1-lMjjv-bs8r7eWibt2qk_Lv518FeYEl</recordid><startdate>19951101</startdate><enddate>19951101</enddate><creator>Dagneaux, C</creator><creator>Liquier, J</creator><creator>Taillandier, E</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951101</creationdate><title>FTIR Study of a Nonclassical dT10dA10-dT10 Intramolecular Triple Helix</title><author>Dagneaux, C ; Liquier, J ; Taillandier, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a269t-defb8339f146c2ea268bac756dee72abff8743be7c2f5ee557f28ae75f6a75173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Base Composition</topic><topic>DNA - chemistry</topic><topic>Molecular Structure</topic><topic>Nucleic Acid Conformation</topic><topic>Poly A - chemistry</topic><topic>Poly T - chemistry</topic><topic>Spectroscopy, Fourier Transform Infrared - methods</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dagneaux, C</creatorcontrib><creatorcontrib>Liquier, J</creatorcontrib><creatorcontrib>Taillandier, E</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dagneaux, C</au><au>Liquier, J</au><au>Taillandier, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FTIR Study of a Nonclassical dT10dA10-dT10 Intramolecular Triple Helix</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1995-11-01</date><risdate>1995</risdate><volume>34</volume><issue>45</issue><spage>14815</spage><epage>14818</epage><pages>14815-14818</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Many early investigations on triple helices have been devoted to the study of the triplex formed by dT*dA-dT base triplets in which the third strand is oriented parallel to the dA strand. 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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Animals Base Composition DNA - chemistry Molecular Structure Nucleic Acid Conformation Poly A - chemistry Poly T - chemistry Spectroscopy, Fourier Transform Infrared - methods Temperature |
| title | FTIR Study of a Nonclassical dT10dA10-dT10 Intramolecular Triple Helix |
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