Murine Thrombopoietin mRNA Levels Are Modulated by Platelet Count
The activity of the c-Mpl ligand on hematopoietic progenitors meets criteria expected for thrombopoietin (TPO). Bioassays have shown that blood TPO levels are inversely related to platelet mass. We sought to identify the molecular basis for this regulation. To determine if TPO mRNA levels respond to...
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| Veröffentlicht in: | Blood 1995-11, Vol.86 (10), p.3668-3675 |
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| creator | McCarty, John M. Sprugel, Katherine H. Fox, Norma E. Sabath, Daniel E. Kaushansky, Kenneth |
| description | The activity of the c-Mpl ligand on hematopoietic progenitors meets criteria expected for thrombopoietin (TPO). Bioassays have shown that blood TPO levels are inversely related to platelet mass. We sought to identify the molecular basis for this regulation. To determine if TPO mRNA levels respond to platelet demand, RNA from selected organs of mice with high, normal, or low platelet counts was subjected to semiquantitative reverse transcriptase-polymerase chain reaction. Although no differences in TPO mRNA levels between control and treated mice could be detected in liver or kidney, TPO-specific bands were more intense after 25 to 30 polymerase chain reaction cycles in marrow-derived mRNA from thrombocytopenic mice. The TPO-specific bands were less intense in thrombocytotic mouse marrow and spleen than control mouse marrow and spleen after 30 cycles. These data support the hypothesis that TPO levels are regulated, at least in part, by modulating mRNA levels in response to platelet demand. |
| doi_str_mv | 10.1182/blood.V86.10.3668.bloodjournal86103668 |
| format | Article |
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Bioassays have shown that blood TPO levels are inversely related to platelet mass. We sought to identify the molecular basis for this regulation. To determine if TPO mRNA levels respond to platelet demand, RNA from selected organs of mice with high, normal, or low platelet counts was subjected to semiquantitative reverse transcriptase-polymerase chain reaction. Although no differences in TPO mRNA levels between control and treated mice could be detected in liver or kidney, TPO-specific bands were more intense after 25 to 30 polymerase chain reaction cycles in marrow-derived mRNA from thrombocytopenic mice. The TPO-specific bands were less intense in thrombocytotic mouse marrow and spleen than control mouse marrow and spleen after 30 cycles. These data support the hypothesis that TPO levels are regulated, at least in part, by modulating mRNA levels in response to platelet demand.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V86.10.3668.bloodjournal86103668</identifier><identifier>PMID: 7579332</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Base Sequence ; Biological and medical sciences ; Blood Platelets - immunology ; Bone Marrow - metabolism ; Bone Marrow Cells ; DNA, Complementary - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Immune Sera - pharmacology ; Kidney - metabolism ; Liver - metabolism ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; Organ Specificity ; Platelet Count ; Polymerase Chain Reaction ; Protein hormones. Growth factors. Cytokines ; Proteins ; Rabbits ; Receptors, Immunologic - physiology ; RNA, Messenger - biosynthesis ; Spleen - metabolism ; Thrombocytopenia - genetics ; Thrombocytopenia - metabolism ; Thrombopoietin - biosynthesis ; Thrombopoietin - genetics</subject><ispartof>Blood, 1995-11, Vol.86 (10), p.3668-3675</ispartof><rights>1995 American Society of Hematology</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4858-ea3e05ac372d8735ac3fb966ec2d458058e174076a394b6b2bc5363a2a1654683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2909223$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7579332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McCarty, John M.</creatorcontrib><creatorcontrib>Sprugel, Katherine H.</creatorcontrib><creatorcontrib>Fox, Norma E.</creatorcontrib><creatorcontrib>Sabath, Daniel E.</creatorcontrib><creatorcontrib>Kaushansky, Kenneth</creatorcontrib><title>Murine Thrombopoietin mRNA Levels Are Modulated by Platelet Count</title><title>Blood</title><addtitle>Blood</addtitle><description>The activity of the c-Mpl ligand on hematopoietic progenitors meets criteria expected for thrombopoietin (TPO). Bioassays have shown that blood TPO levels are inversely related to platelet mass. We sought to identify the molecular basis for this regulation. To determine if TPO mRNA levels respond to platelet demand, RNA from selected organs of mice with high, normal, or low platelet counts was subjected to semiquantitative reverse transcriptase-polymerase chain reaction. Although no differences in TPO mRNA levels between control and treated mice could be detected in liver or kidney, TPO-specific bands were more intense after 25 to 30 polymerase chain reaction cycles in marrow-derived mRNA from thrombocytopenic mice. The TPO-specific bands were less intense in thrombocytotic mouse marrow and spleen than control mouse marrow and spleen after 30 cycles. These data support the hypothesis that TPO levels are regulated, at least in part, by modulating mRNA levels in response to platelet demand.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blood Platelets - immunology</subject><subject>Bone Marrow - metabolism</subject><subject>Bone Marrow Cells</subject><subject>DNA, Complementary - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Immune Sera - pharmacology</subject><subject>Kidney - metabolism</subject><subject>Liver - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity</subject><subject>Platelet Count</subject><subject>Polymerase Chain Reaction</subject><subject>Protein hormones. Growth factors. Cytokines</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Receptors, Immunologic - physiology</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Spleen - metabolism</subject><subject>Thrombocytopenia - genetics</subject><subject>Thrombocytopenia - metabolism</subject><subject>Thrombopoietin - biosynthesis</subject><subject>Thrombopoietin - genetics</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMtKxDAUhoMoOo4-gtCFuOuYS3Ppchi8wXhB1G1I0zMYSZsxaQXf3tYZZuXGVQ7_-fhz-BCaETwjRNHLyodQz96UGBMmhJr9Jh-hj63xShA8hntoQjhVOcYU76MJxljkRSnJETpO6QNjUjDKD9Gh5LJkjE7Q_L6ProXs5T2Gpgrr4KBzbdY8P8yzJXyBT9k8QnYf6t6bDuqs-s6exslDly1C33Yn6GBlfILT7TtFr9dXL4vbfPl4c7eYL3NbKK5yMAwwN5ZJWivJxmlVlUKApXXBFeYKiCywFIaVRSUqWlnOBDPUEMELodgUXWx61zF89pA63bhkwXvTQuiTllIoqngxgNcb0MaQUoSVXkfXmPitCdajS_1rTg8ux2TUpv9yORSdbX_sqwbqXc1W3rA_3-5NssavommtSzuMlriklA3Y0wYbZMKXg6iTddBaqF0E2-k6uP9e9gOhXJ5T</recordid><startdate>19951115</startdate><enddate>19951115</enddate><creator>McCarty, John M.</creator><creator>Sprugel, Katherine H.</creator><creator>Fox, Norma E.</creator><creator>Sabath, Daniel E.</creator><creator>Kaushansky, Kenneth</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951115</creationdate><title>Murine Thrombopoietin mRNA Levels Are Modulated by Platelet Count</title><author>McCarty, John M. ; Sprugel, Katherine H. ; Fox, Norma E. ; Sabath, Daniel E. ; Kaushansky, Kenneth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4858-ea3e05ac372d8735ac3fb966ec2d458058e174076a394b6b2bc5363a2a1654683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blood Platelets - immunology</topic><topic>Bone Marrow - metabolism</topic><topic>Bone Marrow Cells</topic><topic>DNA, Complementary - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Immune Sera - pharmacology</topic><topic>Kidney - metabolism</topic><topic>Liver - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity</topic><topic>Platelet Count</topic><topic>Polymerase Chain Reaction</topic><topic>Protein hormones. Growth factors. Cytokines</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Receptors, Immunologic - physiology</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Spleen - metabolism</topic><topic>Thrombocytopenia - genetics</topic><topic>Thrombocytopenia - metabolism</topic><topic>Thrombopoietin - biosynthesis</topic><topic>Thrombopoietin - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McCarty, John M.</creatorcontrib><creatorcontrib>Sprugel, Katherine H.</creatorcontrib><creatorcontrib>Fox, Norma E.</creatorcontrib><creatorcontrib>Sabath, Daniel E.</creatorcontrib><creatorcontrib>Kaushansky, Kenneth</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McCarty, John M.</au><au>Sprugel, Katherine H.</au><au>Fox, Norma E.</au><au>Sabath, Daniel E.</au><au>Kaushansky, Kenneth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Murine Thrombopoietin mRNA Levels Are Modulated by Platelet Count</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1995-11-15</date><risdate>1995</risdate><volume>86</volume><issue>10</issue><spage>3668</spage><epage>3675</epage><pages>3668-3675</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>The activity of the c-Mpl ligand on hematopoietic progenitors meets criteria expected for thrombopoietin (TPO). Bioassays have shown that blood TPO levels are inversely related to platelet mass. We sought to identify the molecular basis for this regulation. To determine if TPO mRNA levels respond to platelet demand, RNA from selected organs of mice with high, normal, or low platelet counts was subjected to semiquantitative reverse transcriptase-polymerase chain reaction. Although no differences in TPO mRNA levels between control and treated mice could be detected in liver or kidney, TPO-specific bands were more intense after 25 to 30 polymerase chain reaction cycles in marrow-derived mRNA from thrombocytopenic mice. The TPO-specific bands were less intense in thrombocytotic mouse marrow and spleen than control mouse marrow and spleen after 30 cycles. These data support the hypothesis that TPO levels are regulated, at least in part, by modulating mRNA levels in response to platelet demand.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>7579332</pmid><doi>10.1182/blood.V86.10.3668.bloodjournal86103668</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Animals Base Sequence Biological and medical sciences Blood Platelets - immunology Bone Marrow - metabolism Bone Marrow Cells DNA, Complementary - genetics Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation Immune Sera - pharmacology Kidney - metabolism Liver - metabolism Mice Mice, Inbred C57BL Molecular Sequence Data Organ Specificity Platelet Count Polymerase Chain Reaction Protein hormones. Growth factors. Cytokines Proteins Rabbits Receptors, Immunologic - physiology RNA, Messenger - biosynthesis Spleen - metabolism Thrombocytopenia - genetics Thrombocytopenia - metabolism Thrombopoietin - biosynthesis Thrombopoietin - genetics |
| title | Murine Thrombopoietin mRNA Levels Are Modulated by Platelet Count |
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