Immunofixation compared with immunoelectrophoresis for the routine characterization of paraprotein disorders
The authors prospectively compared immunofixation electrophoresis (IFE) with immunoelectrophoresis (IEP) for the evaluation of paraproteins in 32 patient specimens. They used a simple modification of their existing agarose protein electrophoresis system for IFE. Thirteen specimens (40%) required IFE...
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| Veröffentlicht in: | American journal of clinical pathology 1987-08, Vol.88 (2), p.198-203 |
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| container_title | American journal of clinical pathology |
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| creator | GERARD, S. K CHEN, K. H KHAYAM-BASHI, H |
| description | The authors prospectively compared immunofixation electrophoresis (IFE) with immunoelectrophoresis (IEP) for the evaluation of paraproteins in 32 patient specimens. They used a simple modification of their existing agarose protein electrophoresis system for IFE. Thirteen specimens (40%) required IFE for paraprotein identification, 18 (56%) were definitive by either method, and 1 (3%) did not show a paraprotein by either method. Low titer and/or avidity with anti-lambda antibody from three of seven suppliers prevented or complicated interpretation of IEP patterns with two of four known lambda paraprotein-containing specimens but had no such effect with IFE. In view of these and various additional technical considerations, the authors suggest IFE for the routine characterization of paraproteins in the serum or urine. |
| doi_str_mv | 10.1093/ajcp/88.2.198 |
| format | Article |
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In view of these and various additional technical considerations, the authors suggest IFE for the routine characterization of paraproteins in the serum or urine.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1093/ajcp/88.2.198</identifier><identifier>PMID: 3618552</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Biological and medical sciences ; Blood Protein Electrophoresis ; Filtration ; Humans ; Immunodeficiencies. Immunoglobulinopathies ; Immunoelectrophoresis ; Immunoglobulinopathies ; Immunopathology ; Medical sciences ; Paraproteinemias - diagnosis ; Paraproteins - analysis ; Paraproteins - immunology</subject><ispartof>American journal of clinical pathology, 1987-08, Vol.88 (2), p.198-203</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c317t-24646fc8f8a2826db17af4df7eb51250e929f0e51f62a5bea810669ebe6280be3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8335995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3618552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GERARD, S. K</creatorcontrib><creatorcontrib>CHEN, K. H</creatorcontrib><creatorcontrib>KHAYAM-BASHI, H</creatorcontrib><title>Immunofixation compared with immunoelectrophoresis for the routine characterization of paraprotein disorders</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>The authors prospectively compared immunofixation electrophoresis (IFE) with immunoelectrophoresis (IEP) for the evaluation of paraproteins in 32 patient specimens. They used a simple modification of their existing agarose protein electrophoresis system for IFE. Thirteen specimens (40%) required IFE for paraprotein identification, 18 (56%) were definitive by either method, and 1 (3%) did not show a paraprotein by either method. Low titer and/or avidity with anti-lambda antibody from three of seven suppliers prevented or complicated interpretation of IEP patterns with two of four known lambda paraprotein-containing specimens but had no such effect with IFE. In view of these and various additional technical considerations, the authors suggest IFE for the routine characterization of paraproteins in the serum or urine.</description><subject>Biological and medical sciences</subject><subject>Blood Protein Electrophoresis</subject><subject>Filtration</subject><subject>Humans</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunoelectrophoresis</subject><subject>Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Medical sciences</subject><subject>Paraproteinemias - diagnosis</subject><subject>Paraproteins - analysis</subject><subject>Paraproteins - immunology</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtr3DAURkVJSCeTLrssaBG684weth7LMrRNIJBNshayfMUo2JYryTTJr6-nY7LS4jscrg5CXynZUaL53r64aa_Uju2oVp_QhuqaV1IydoE2hBBWaSr5Z3Sd8wshlClSX6ErLqhqGrZB_f0wzGP04dWWEEfs4jDZBB3-G8oRh_8j9OBKitMxJsghYx8TLkfAKc4ljIDd0SbrCqTwfpZEjxeJnVIsEEbchRxTBynfoEtv-wxf1neLnn_9fDrcVQ-Pv-8PPx4qx6ksFatFLbxTXlmmmOhaKq2vOy-hbShrCGimPYGGesFs04JVlAihoQWxfK8FvkXfz97lgj8z5GKGkB30vR0hztlIKaTSmi5gdQZdijkn8GZKYbDpzVBiTnXNqa5RyjCz1F34b6t4bgfoPug157LfrrvNzvY-2dGF_IEpzhutG_4PesaGjA</recordid><startdate>19870801</startdate><enddate>19870801</enddate><creator>GERARD, S. K</creator><creator>CHEN, K. H</creator><creator>KHAYAM-BASHI, H</creator><general>American Society of Clinical Pathologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870801</creationdate><title>Immunofixation compared with immunoelectrophoresis for the routine characterization of paraprotein disorders</title><author>GERARD, S. K ; CHEN, K. H ; KHAYAM-BASHI, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c317t-24646fc8f8a2826db17af4df7eb51250e929f0e51f62a5bea810669ebe6280be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Biological and medical sciences</topic><topic>Blood Protein Electrophoresis</topic><topic>Filtration</topic><topic>Humans</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunoelectrophoresis</topic><topic>Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Medical sciences</topic><topic>Paraproteinemias - diagnosis</topic><topic>Paraproteins - analysis</topic><topic>Paraproteins - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GERARD, S. K</creatorcontrib><creatorcontrib>CHEN, K. H</creatorcontrib><creatorcontrib>KHAYAM-BASHI, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GERARD, S. K</au><au>CHEN, K. H</au><au>KHAYAM-BASHI, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunofixation compared with immunoelectrophoresis for the routine characterization of paraprotein disorders</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>1987-08-01</date><risdate>1987</risdate><volume>88</volume><issue>2</issue><spage>198</spage><epage>203</epage><pages>198-203</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><coden>AJCPAI</coden><abstract>The authors prospectively compared immunofixation electrophoresis (IFE) with immunoelectrophoresis (IEP) for the evaluation of paraproteins in 32 patient specimens. They used a simple modification of their existing agarose protein electrophoresis system for IFE. Thirteen specimens (40%) required IFE for paraprotein identification, 18 (56%) were definitive by either method, and 1 (3%) did not show a paraprotein by either method. Low titer and/or avidity with anti-lambda antibody from three of seven suppliers prevented or complicated interpretation of IEP patterns with two of four known lambda paraprotein-containing specimens but had no such effect with IFE. In view of these and various additional technical considerations, the authors suggest IFE for the routine characterization of paraproteins in the serum or urine.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>3618552</pmid><doi>10.1093/ajcp/88.2.198</doi><tpages>6</tpages></addata></record> |
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| ispartof | American journal of clinical pathology, 1987-08, Vol.88 (2), p.198-203 |
| issn | 0002-9173 1943-7722 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | Biological and medical sciences Blood Protein Electrophoresis Filtration Humans Immunodeficiencies. Immunoglobulinopathies Immunoelectrophoresis Immunoglobulinopathies Immunopathology Medical sciences Paraproteinemias - diagnosis Paraproteins - analysis Paraproteins - immunology |
| title | Immunofixation compared with immunoelectrophoresis for the routine characterization of paraprotein disorders |
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