Analysis of human lymphocyte transformation responses to graded doses of T cell mitogens by curve fitting
The between-group comparison of complete lymphocyte transformation dose-response curves is complex. We have therefore derived a mathematical model of the dose-response characteristics of human mononuclear cells to stimulation by concanavalin A (ConA) and purified phytohaemagglutinin (PHA), in order...
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| Veröffentlicht in: | Journal of immunological methods 1987-08, Vol.102 (1), p.109-117 |
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| container_end_page | 117 |
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| container_title | Journal of immunological methods |
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| creator | Cason, J. Chinn, S. Ainley, C.C. Wolstencroft, R.A. Thompson, R.P.H. |
| description | The between-group comparison of complete lymphocyte transformation dose-response curves is complex. We have therefore derived a mathematical model of the dose-response characteristics of human mononuclear cells to stimulation by concanavalin A (ConA) and purified phytohaemagglutinin (PHA), in order to simplify such analyses.
This model describes dose-response curves in terms of the magnitude of the peak response, the dose of mitogen that elicits the peak and an estimate of the range of mitogen doses which induce a response.
Responses to ConA were described by the model more precisely than those to PHA. Furthermore, use of the model revealed differences between anorexia nervosa patients and healthy subjects in terms of the dose of mitogen necessary to elicit a peak response and the range of mitogen concentrations producing a response.
It is proposed that this form of mathematical treatment may be of use for the comparison of lymphocyte transformation dose-response curves and for the valid rejection of suspect results. |
| doi_str_mv | 10.1016/S0022-1759(87)80016-9 |
| format | Article |
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This model describes dose-response curves in terms of the magnitude of the peak response, the dose of mitogen that elicits the peak and an estimate of the range of mitogen doses which induce a response.
Responses to ConA were described by the model more precisely than those to PHA. Furthermore, use of the model revealed differences between anorexia nervosa patients and healthy subjects in terms of the dose of mitogen necessary to elicit a peak response and the range of mitogen concentrations producing a response.
It is proposed that this form of mathematical treatment may be of use for the comparison of lymphocyte transformation dose-response curves and for the valid rejection of suspect results.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/S0022-1759(87)80016-9</identifier><identifier>PMID: 3497987</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Anorexia nervosa ; Anorexia Nervosa - immunology ; Applied sciences ; Concanavalin A - pharmacology ; curve fitting ; Dose-Response Relationship, Drug ; Exact sciences and technology ; Female ; Humans ; Interleukin-2 ; Lymphocyte Activation ; Lymphocyte transformation response ; Mathematics ; Mitogen dose-response curve ; Mitogens - pharmacology ; Models, Biological ; Other techniques and industries ; Phytohemagglutinins - pharmacology ; T-Lymphocytes - immunology</subject><ispartof>Journal of immunological methods, 1987-08, Vol.102 (1), p.109-117</ispartof><rights>1987 Elsevier Science Publishers B.V. (Biomedical Division)</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-e0b4f71c005ea6a343c0d78ea022da1e890616a3969a28dd998c2a6b575a02473</citedby><cites>FETCH-LOGICAL-c389t-e0b4f71c005ea6a343c0d78ea022da1e890616a3969a28dd998c2a6b575a02473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0022-1759(87)80016-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7844883$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3497987$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cason, J.</creatorcontrib><creatorcontrib>Chinn, S.</creatorcontrib><creatorcontrib>Ainley, C.C.</creatorcontrib><creatorcontrib>Wolstencroft, R.A.</creatorcontrib><creatorcontrib>Thompson, R.P.H.</creatorcontrib><title>Analysis of human lymphocyte transformation responses to graded doses of T cell mitogens by curve fitting</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>The between-group comparison of complete lymphocyte transformation dose-response curves is complex. We have therefore derived a mathematical model of the dose-response characteristics of human mononuclear cells to stimulation by concanavalin A (ConA) and purified phytohaemagglutinin (PHA), in order to simplify such analyses.
This model describes dose-response curves in terms of the magnitude of the peak response, the dose of mitogen that elicits the peak and an estimate of the range of mitogen doses which induce a response.
Responses to ConA were described by the model more precisely than those to PHA. Furthermore, use of the model revealed differences between anorexia nervosa patients and healthy subjects in terms of the dose of mitogen necessary to elicit a peak response and the range of mitogen concentrations producing a response.
It is proposed that this form of mathematical treatment may be of use for the comparison of lymphocyte transformation dose-response curves and for the valid rejection of suspect results.</description><subject>Anorexia nervosa</subject><subject>Anorexia Nervosa - immunology</subject><subject>Applied sciences</subject><subject>Concanavalin A - pharmacology</subject><subject>curve fitting</subject><subject>Dose-Response Relationship, Drug</subject><subject>Exact sciences and technology</subject><subject>Female</subject><subject>Humans</subject><subject>Interleukin-2</subject><subject>Lymphocyte Activation</subject><subject>Lymphocyte transformation response</subject><subject>Mathematics</subject><subject>Mitogen dose-response curve</subject><subject>Mitogens - pharmacology</subject><subject>Models, Biological</subject><subject>Other techniques and industries</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>T-Lymphocytes - immunology</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v3CAQhlHVKN2k_QmROFRVc3AK_gJOVRT1S4qUQ9IzYmG8obJhy-BI_vfF2dVee0LMPC8zPIRccXbDGe-_PDJW1xUXnfosxbVkpVapN2TDpagroVj3lmxOyDtygfiHFYj17JycN60SSooN8bfBjAt6pHGgz_NkAh2Xaf8c7ZKB5mQCDjFNJvsYaALcx4CANEe6S8aBoy6u9xJ-ohbGkU4-xx0EpNuF2jm9AB18zj7s3pOzwYwIH47nJfn9_dvT3c_q_uHHr7vb-8o2UuUK2LYdBLeMdWB607SNZU5IMOUrznCQivW81FWvTC2dU0ra2vTbTnQFaUVzST4d3t2n-HcGzHryuK5mAsQZtRC9aETTFLA7gDZFxASD3ic_mbRozvSqWL8q1qs_LYV-VaxVyV0dB8zbCdwpdXRa-h-PfYPWjENxaD2eMCHbVsp1_NcDBkXGi4ek0XoIFpxPYLN20f9nkX-scpk5</recordid><startdate>19870824</startdate><enddate>19870824</enddate><creator>Cason, J.</creator><creator>Chinn, S.</creator><creator>Ainley, C.C.</creator><creator>Wolstencroft, R.A.</creator><creator>Thompson, R.P.H.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870824</creationdate><title>Analysis of human lymphocyte transformation responses to graded doses of T cell mitogens by curve fitting</title><author>Cason, J. ; Chinn, S. ; Ainley, C.C. ; Wolstencroft, R.A. ; Thompson, R.P.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-e0b4f71c005ea6a343c0d78ea022da1e890616a3969a28dd998c2a6b575a02473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Anorexia nervosa</topic><topic>Anorexia Nervosa - immunology</topic><topic>Applied sciences</topic><topic>Concanavalin A - pharmacology</topic><topic>curve fitting</topic><topic>Dose-Response Relationship, Drug</topic><topic>Exact sciences and technology</topic><topic>Female</topic><topic>Humans</topic><topic>Interleukin-2</topic><topic>Lymphocyte Activation</topic><topic>Lymphocyte transformation response</topic><topic>Mathematics</topic><topic>Mitogen dose-response curve</topic><topic>Mitogens - pharmacology</topic><topic>Models, Biological</topic><topic>Other techniques and industries</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cason, J.</creatorcontrib><creatorcontrib>Chinn, S.</creatorcontrib><creatorcontrib>Ainley, C.C.</creatorcontrib><creatorcontrib>Wolstencroft, R.A.</creatorcontrib><creatorcontrib>Thompson, R.P.H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cason, J.</au><au>Chinn, S.</au><au>Ainley, C.C.</au><au>Wolstencroft, R.A.</au><au>Thompson, R.P.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of human lymphocyte transformation responses to graded doses of T cell mitogens by curve fitting</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1987-08-24</date><risdate>1987</risdate><volume>102</volume><issue>1</issue><spage>109</spage><epage>117</epage><pages>109-117</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>The between-group comparison of complete lymphocyte transformation dose-response curves is complex. We have therefore derived a mathematical model of the dose-response characteristics of human mononuclear cells to stimulation by concanavalin A (ConA) and purified phytohaemagglutinin (PHA), in order to simplify such analyses.
This model describes dose-response curves in terms of the magnitude of the peak response, the dose of mitogen that elicits the peak and an estimate of the range of mitogen doses which induce a response.
Responses to ConA were described by the model more precisely than those to PHA. Furthermore, use of the model revealed differences between anorexia nervosa patients and healthy subjects in terms of the dose of mitogen necessary to elicit a peak response and the range of mitogen concentrations producing a response.
It is proposed that this form of mathematical treatment may be of use for the comparison of lymphocyte transformation dose-response curves and for the valid rejection of suspect results.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3497987</pmid><doi>10.1016/S0022-1759(87)80016-9</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1987-08, Vol.102 (1), p.109-117 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anorexia nervosa Anorexia Nervosa - immunology Applied sciences Concanavalin A - pharmacology curve fitting Dose-Response Relationship, Drug Exact sciences and technology Female Humans Interleukin-2 Lymphocyte Activation Lymphocyte transformation response Mathematics Mitogen dose-response curve Mitogens - pharmacology Models, Biological Other techniques and industries Phytohemagglutinins - pharmacology T-Lymphocytes - immunology |
| title | Analysis of human lymphocyte transformation responses to graded doses of T cell mitogens by curve fitting |
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