Rabbit bone marrow glucose-6-phosphate dehydrogenase during erythroid cell development
Studies were carried out on glucose-6-phosphate dehydrogenase (G6P-DH) during the differentiation of rabbit bone marrow erythroid cells. It was found that G6P-DH, although displaying a 7-fold activity decrease, did not change the relative amounts of its three dimeric forms. Using homogeneous enzyme...
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| Veröffentlicht in: | Molecular and cellular biochemistry 1987-06, Vol.75 (2), p.85-92 |
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| creator | Ninfali, P Palma, F Fornaini, G |
| description | Studies were carried out on glucose-6-phosphate dehydrogenase (G6P-DH) during the differentiation of rabbit bone marrow erythroid cells. It was found that G6P-DH, although displaying a 7-fold activity decrease, did not change the relative amounts of its three dimeric forms. Using homogeneous enzyme preparations, we observed that from dividing to non-dividing erythroblasts the following properties remained constant: V max dependence on pH and temperature, Km for G6P dependence on pH, heat stability, 2-deoxy glucose-6-phosphate utilization, molecular weight, while the Km for NADP significantly increased in non-dividing erythroblasts. These results indicate that no shift towards the oxidized form of the enzyme and no substantial modifications of the protein take place during cell differentiation. |
| doi_str_mv | 10.1007/BF00229896 |
| format | Article |
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It was found that G6P-DH, although displaying a 7-fold activity decrease, did not change the relative amounts of its three dimeric forms. Using homogeneous enzyme preparations, we observed that from dividing to non-dividing erythroblasts the following properties remained constant: V max dependence on pH and temperature, Km for G6P dependence on pH, heat stability, 2-deoxy glucose-6-phosphate utilization, molecular weight, while the Km for NADP significantly increased in non-dividing erythroblasts. These results indicate that no shift towards the oxidized form of the enzyme and no substantial modifications of the protein take place during cell differentiation.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/BF00229896</identifier><identifier>PMID: 3627110</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Bone Marrow - enzymology ; Cell Differentiation ; Erythroblasts - cytology ; Erythroblasts - enzymology ; Erythropoiesis ; Glucosephosphate Dehydrogenase - blood ; Hemoglobins - analysis ; Kinetics ; Rabbits</subject><ispartof>Molecular and cellular biochemistry, 1987-06, Vol.75 (2), p.85-92</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3627110$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ninfali, P</creatorcontrib><creatorcontrib>Palma, F</creatorcontrib><creatorcontrib>Fornaini, G</creatorcontrib><title>Rabbit bone marrow glucose-6-phosphate dehydrogenase during erythroid cell development</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>Studies were carried out on glucose-6-phosphate dehydrogenase (G6P-DH) during the differentiation of rabbit bone marrow erythroid cells. 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These results indicate that no shift towards the oxidized form of the enzyme and no substantial modifications of the protein take place during cell differentiation.</description><subject>Animals</subject><subject>Bone Marrow - enzymology</subject><subject>Cell Differentiation</subject><subject>Erythroblasts - cytology</subject><subject>Erythroblasts - enzymology</subject><subject>Erythropoiesis</subject><subject>Glucosephosphate Dehydrogenase - blood</subject><subject>Hemoglobins - analysis</subject><subject>Kinetics</subject><subject>Rabbits</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0EFLw0AQBeBFlFqrF-9CTh6E6Ew2u-setVgVCoKo15DNTppImo27idJ_b0qLnoaBj8fMY-wc4RoB1M39AiBJ9K2WB2yKQvE41agP2RQ4QHyLSh2zkxA-AUaOOGETLhOFCFP28ZobU_eRcS1F69x79xOtmqFwgWIZd5ULXZX3FFmqNta7FbV5GLfB1-0qIr_pK-9qGxXUNKP5psZ1a2r7U3ZU5k2gs_2csffFw9v8KV6-PD7P75ZxkaTYxyUHJa22QpRQpLbQUpGR0oAWWuTapCgEamHJSmsTnmoDiVJaYGokcVPyGbvc5XbefQ0U-mxdh-0xeUtuCJlSUnGe4givdrDwLgRPZdb5evx3kyFk2xKz_xJHfLFPHcya7B_dt8Z_AefIbHA</recordid><startdate>198706</startdate><enddate>198706</enddate><creator>Ninfali, P</creator><creator>Palma, F</creator><creator>Fornaini, G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198706</creationdate><title>Rabbit bone marrow glucose-6-phosphate dehydrogenase during erythroid cell development</title><author>Ninfali, P ; Palma, F ; Fornaini, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c241t-f3076d9d55f0c4dc967eb66b09595a9b4155195ded6dd2349b02779514b6e3bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Bone Marrow - enzymology</topic><topic>Cell Differentiation</topic><topic>Erythroblasts - cytology</topic><topic>Erythroblasts - enzymology</topic><topic>Erythropoiesis</topic><topic>Glucosephosphate Dehydrogenase - blood</topic><topic>Hemoglobins - analysis</topic><topic>Kinetics</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ninfali, P</creatorcontrib><creatorcontrib>Palma, F</creatorcontrib><creatorcontrib>Fornaini, G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ninfali, P</au><au>Palma, F</au><au>Fornaini, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rabbit bone marrow glucose-6-phosphate dehydrogenase during erythroid cell development</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1987-06</date><risdate>1987</risdate><volume>75</volume><issue>2</issue><spage>85</spage><epage>92</epage><pages>85-92</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Studies were carried out on glucose-6-phosphate dehydrogenase (G6P-DH) during the differentiation of rabbit bone marrow erythroid cells. 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| ispartof | Molecular and cellular biochemistry, 1987-06, Vol.75 (2), p.85-92 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Bone Marrow - enzymology Cell Differentiation Erythroblasts - cytology Erythroblasts - enzymology Erythropoiesis Glucosephosphate Dehydrogenase - blood Hemoglobins - analysis Kinetics Rabbits |
| title | Rabbit bone marrow glucose-6-phosphate dehydrogenase during erythroid cell development |
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